Proteomic analysis of differentiating neuroblastoma cells treated with sub-lethal neurite inhibitory concentrations of diazinon: Identification of novel biomarkers of effect

Toxicology and Applied Pharmacology

Volumn 240, Issue 2, 2009, Pages 159-165

  Harris, W ^a   Sachana, M ^b   Flaskos, J ^b   Hargreaves, A J ^a  

^a NOTTINGHAM TRENT UNIVERSITY   (United Kingdom)

^b ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Cell differentiation; Diazinon; Neurite outgrowth; Neuroblastoma; Neurotoxicity; Organophosphate; Proteomics

Indexed keywords

CHAPERONE; COFILIN; DIMPYLATE;

ANIMAL CELL; ARTICLE; CELL METABOLISM; CELL SURVIVAL; CONTROLLED STUDY; MICROFILAMENT; MOUSE; NERVE CELL DIFFERENTIATION; NEUROBLASTOMA CELL; NEUROTOXICITY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEOMICS; UPREGULATION;

ACTIN DEPOLYMERIZING FACTORS; ANIMALS; BIOLOGICAL MARKERS; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL LINE, TUMOR; CELL SURVIVAL; CHOLINESTERASE INHIBITORS; CYTOSKELETON; DIAZINON; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLUORESCENT ANTIBODY TECHNIQUE; INSECTICIDES; MICE; MOLECULAR CHAPERONES; NERVE TISSUE PROTEINS; NEURITES; NEUROBLASTOMA; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 70349233759     PISSN: 0041008X     EISSN: 10960333     Source Type: Journal    
DOI: 10.1016/j.taap.2009.07.028     Document Type: Article

References (60)

1. Abou-Donia M.B. The cytoskeleton as a target for organophosphorous ester-induced delayed neurotoxicity (OPIDN). Chem. Biol. Interact. 87 (1993) 383-393
2. Abou-Donia M.B., and Lapadula D.M. Mechanisms of organophosphorus ester-induced delayed neurotoxicity: Type I and Type II. Ann. Rev. Pharmacol. Toxicol. 30 (1990) 405-440
3. Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological Profile for Diazinon (2008), U.S. Department of Health and Human Services
4. Australia Pesticides and Veterinary Medicines Authority (APVMA), 2007. APVMA suspends the use of Diazinon for Sheep Dipping and Jetting. Media Release 9th May 2007. http://www.apvma.gov.au/media/downloads/mr0704_diazinon.pdf.
5. Axelrad J.C., Howard C.V., and McLean W.G. Interactions between pesticides and components of pesticide formulations in an in vitro neurotoxicity test. Toxicology 173 (2002) 259-268
6. Axelrad J.C., Howard C.V., and McLean W.G. The effects of acute pesticide exposure on neuroblastoma cells chronically exposed to diazinon. Toxicology 185 (2003) 67-78
7. In: Berg J.M., Tymoczko J.L., and Stryer L. (Eds). Biochemistry. 6th ed. (2006), W.H. Freeman and Co., New York 463-469
8. Bukau B., Weissman J., and Horwich A. Molecular chaperones and protein quality control. Cell 125 (2006) 443-451
9. Buratti F.M., Volpe M.T., Meneguz A., Vittozzi L., and Testai E. CYP-specific bioactivation of 4 organophosphorothioate pesticides by human liver microsomes. Toxicol. Appl. Pharmacol. 186 (2003) 143-154
10. Camby I., Le Mercier M., Lefranc F., and Kiss R. Galectin-1: a small protein with major functions. Glycobiology 16 (2006) 137R-157R
11. Carlier M.F., Laurent V., Santolini J., Melki R., Didry D., Xia G.X., Hong Y., Chua N.H., and Pantaloni D. Actin depolymerising factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin based motility. J. Cell. Biol. 136 (1997) 1307-1322
12. Chambers H. Organophosphate compounds: an overview. Organophosphates: Chemistry, Fate and Effects (1992), Academic Press, New York 3-17
13. Chow E., Seiber J.N., and Wilson B.W. Isophenos and an in vitro assay for delayed neuropathic potential. Toxicol. Appl. Pharmacol. 83 (1986) 178-183
14. Clarke Z. Diazinon. In: Enna S.J., and Bylund D.B. (Eds). X-Pharm: The Comprehensive Pharmacology Reference (2007), Elsevier Ltd
15. Committee on Toxicology (COT) of Chemicals in Food, Consumer Products and the Environment. Organophosphates (2007), Department of Health, UK
16. COT, 1999. Organophosphates. A Report of the Committee on Toxicology of Chemicals in Food, Consumer Products and the Environment. UK Department of Health, London.
17. De Blaquiere G.E., Waters L., Blain P.G., and Williams F.M. Electrophysiological and biochemical effects of single and multiple doses of the organophosphate diazinon in the mouse. Toxicol. Appl. Pharmacol. 166 (2000) 81-91
18. Department of Primary Industries (DPI). Sheep Body Lice: Control and Eradication (2007), Agriculture Notes of the DPI (State of Victoria) AG 1110
19. Environmental Protection Agency (EPA), 2000. Annual Report. Office of Pesticide Programs. http://www.epa.gov/oppfead1/annual/2000/2000annual.pdf.
20. EPA, 2006. Reregistration Eligibility Decision for Diazinon. http://www.epa.gov/pesticides/reregistration/REDs/diazinon_red.pdf.
21. European Commission (EC) Commission decision of 6 June 2007 concerning the non-inclusion of diazinon in Annex I to Council Directive 91/414/EEC and the withdrawal of authorisations for plant protection agents containing that substance. Official Journal of the European Union 148, 9-10.
22. Flaskos J., McLean W.G., Fowler M.J., and Hargreaves A.J. Tricresyl phosphate inhibits the formation of axon-like processes and disrupts neurofilaments in cultured mouse N2a and rat PC12 cells. Neurosci. Lett. 242 (1998) 101-104
23. Flaskos J., Harris W., Sachana M., Muñoz D., Tack J., and Hargreaves A.J. The effects of diazinon and cypermethrin on the differentiation of neuronal and glial cell lines. Toxicol. Appl. Pharmacol. 219 (2007) 172-180
24. Gonzalez M.F., Shiraishi K., Hisanaga K., Sagar S.M., Mandabach M., and Sharp F.R. Heat shock proteins as markers of neural injury. Mol. Brain Res. 6 (1989) 93-100
25. Gungabissoon R., and Bamburg J.R. Regulation of growth cone actin dynamics by ADF/cofilin. J. Histochem. Cytochem. 51 (2003) 411-420
26. Hamos J.E., Oblas B., Pulaski-Salo D., Welch W.J., Bole D.G., and Drachman D.A. Expression of heat shock proteins in Alzheimer's disease. Neurology 41 (1991) 345-350
27. Hargreaves A.J., Fowler M.J., Sachana M., Flaskos J., Bountouri M., Coutts I.C., Glynn P., Harris W., and McLean W.G. Inhibition of neurite outgrowth in differentiating mouse N2a neuroblastoma cells by phenyl saligenin phosphate: effects on MAP kinase (ERK 1/2) activation, neurofilament heavy chain phosphorylation and neuropathy target esterase activity. Biochem. Pharmacol. 71 (2006) 1240-1247
28. Hartl F.U., and Hayer-Hartl M. Molecular chaperones in the cytosol: from nascent chain to folded protein. Science 295 (2002) 1852-1858
29. Hatjian B.A., Mutch E., Williams F.M., Blain P.G., and Edwards J.W. Cytogenetic response without changes in peripheral cholinesterase following exposure to a sheep dip containing diazinon in vivo and in vitro. Mutat. Res. 472 (2000) 85-92
30. HHS. Toxicological Profile for Diazinon (Update). Public Health Service, Agency for Toxic Substances and Disease Registry (1996), U.S. Department of Health and Human Services, Atlanta, GA
31. HSE, 2002. Risk assessment for acute toxicity from sheep ectoparasite treatments, including organophosphates (OPs) used in plunge dipping.
32. Karalliedde L., Baker D., and Marrs T.C. Organophosphate-induced intermediate syndrome: aetiology and relationships with myopathy. Toxicol. Rev. 25 (2006) 1-14
33. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
34. Larkin D.J., and Tjeedema R.S. Fate and effects of diazinon. Rev. Environ. Contam. Toxicol. 166 (2000) 49-82
35. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
36. Luo S., Mao C., Lee B., and Lee A.S. GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol. Cell. Biol. 26 (2006) 5688-5697
37. Marinovich M., Ghilardi F., and Galli C.L. Effect of pesticide mixtures on in vitro nervous cells: comparison with single pesticides. Toxicology 108 (1996) 201-206
38. McGough A., and Chiu W. ADF/cofilin weakens lateral contacts in the actin filament. J. Mol. Biol. 291 (1999) 513-519
39. McGraw J., Gaudet A.D., Oschipok L.W., Kadoya T., Horie H., Steeves J.D., Tetzlaff W., and Ramer M.S. Regulation of glial and neuronal galectin-1 expression by peripheral and central axotomy of rat primary afferent neurons. Exp. Neurol. 195 (2005) 103-114
40. Nutley B.P., and Crocker J. Biological monitoring of workers occupationally exposed to organophosphorous pesticides. Pestic. Sci. 39 (1993) 315-322
41. In: Olsen K.R. (Ed). Poisoning and Drug Overdose (1998), Appleton and Lange, Stamford Connecticut
42. Paraoanu L.E., Mocko J.B., Becker-Roeck M., Smidek-Huhn J., and Layer P.G. Exposure to diazinon alters in vitro retinogenesis: retinospheroid morphology, development of chicken retinal cell types, and gene expression. Toxicol. Sci. 89 (2006) 214-324
43. Pilkington A., Buchanan D., Jamal G.A., Gillham R., Hamsen S., Kidd M., Hurley J.F., and Soutar C.A. An epidemiological study of the relations between exposure to organophosphate pesticides and indices of chronic peripheral neuropathy and neuropsychological abnormalities in sheep farmers and dippers. Occup. Environ. Med. 58 (2001) 702-710
44. Pope C.N. Organophosphorous pesticides: do they all have the same mechanism of toxicity?. J. Toxicol. Environ. Health 2 (1999) 161-181
45. Qiao D., Seidler F.J., and Slotkin T.A. Developmental neurotoxicity of chlorpyrifos modeled in vitro: comparative effects of metabolites and other cholinesterase inhibitors on DNA synthesis in PC12 and C6 cells. Environ. Health Perspect. 109 (2001) 909-913
46. Reddy R.K., Mao C., Baumeister P., Austin R.C., and Kaufman R.J. Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors. J. Biol. Chem. 278 (2003) 20915-20924
47. Rees H. Exposure to sheep dip and the incidence of acute symptoms in a group of Welsh sheep farmers. Occup. Environ. Med. 53 (1996) 258-263
48. Richards P., Johnson M., Ray D.E., and Walker C. Novel targets for organophosphorus compounds. Chem. Biol. Interact. 119-120 (1999) 503-511
49. Sachana M., Flaskos J., Alexaki E., Glynn P., and Hargreaves A.J. The toxicity of chlorpyrifos towards differentiating mouse N2a neuroblastoma cells. Toxicol. In Vitro 15 (2001) 105-108
50. Sachana M., Flaskos J., Alexaki E., and Hargreaves A.J. Inhibition of neurite outgrowth in N2a cells by leptophos and carbaryl: effects on neurofilament heavy chain, GAP-43 and HSP-70. Toxicol. In Vitro 17 (2003) 115-120
51. Sakaguchi M., Shingo T., Shimazaki T., Hokano H.J., Shiwa M., Ishibashi S., Oguro H., Ninomiya M., Kadoya T., Horie H., Shibuya A., Mizusawa H., Poirier F., Nakauchi H., Sawamoto K., and Okano H. A carbohydrate binding protein, galectin-1, promotes proliferation of adult neural stem cells. Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 7112-7117
52. Slotkin T.A., and Seidler F.J. Developmental neurotoxicants target differentiation into the serotonin phenotype: chlorpyrifos, diazinon, dieldrin and divalent nickel. Toxicol. Appl. Pharmacol. 233 (2008) 211-219
53. Slotkin T.A., Levin E.D., and Seidler F.J. Comparative developmental neurotoxicity of organophosphate insecticides: effects on brain development are separable from systemic toxicity. Env. Health Perspect. 114 (2006) 746-751
54. Slotkin T.A., Ryde T.I., Levin E.D., and Seidler F.J. Developmental neurotoxicity of low dose diazinon exposure of neonatal rats: effects on serotonin systems in adolescence and adulthood. Brain Res. Bull. 75 (2008) 640-647
55. Smulders C.J.G.M., Tjerk T.J.H., Vailati S., van Kleef R.G.D.M., and Vijverberg H.P.M. Block of neuronal nicotinic acetyl choline receptors by organophosphate insecticides. Toxicol. Sci. 82 (2004) 545-554
56. Solomon C., Poole J., Palmer K.T., Peveler R., and Coggon D. Neuropsychiatric symptoms in past users of sheep dip and other pesticides. Occup. Environ. Med 64 (2007) 259-266
57. Suzuki M., Takamura Y., Maeno M., Tochinai S., Iyaguchi D., Tanaka I., Nishihira J., and Ishibashi T. Xenopus laevis macrophage migration inhibitory factor is essential for axis formation and neural development. J. Biol. Chem. 279 (2004) 21406-21414
58. Towbin S., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U. S. A. 76 (1979) 4350-4354
59. Watterson A.E. Regulating pesticides in the UK: a case study of risk management problems relating to the organophosphate diazinon. Toxicol. Lett. 107 (1999) 241-248
60. World Health Organisation (WHO). Diazinon. Joint report by WHO, the International Labour Organisation and the United Nations Environment programme. Environ. Health Criteria 198 (1998)