Use of stabilizing mutations to engineer a charged group within a ligand-binding hydrophobic cavity in T4 lysozyme

Biochemistry

Volumn 48, Issue 37, 2009, Pages 8842-8851

  Liu, Lijun ^a   Baase, Walter A ^a   Michael, Miya M ^a   Matthews, Brian W ^a  

^a UNIVERSITY OF OREGON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BURIED CHARGES; CAVITY WALL; CHARGED GROUPS; CRYSTAL STRUCTURE DETERMINATION; HYDROGEN BOND NETWORKS; HYDROPHOBIC CAVITIES; HYDROPHOBIC CORE; ISOTHERMAL TITRATION CALORIMETRY; LARGE DISKS; LIGAND BINDING; NON-POLAR; NON-POLAR LIGANDS; NONPOLAR ENVIRONMENT; ORDERED WATER; SIDE CHAINS; T4 LYSOZYME; WATER MOLECULE;

CRYSTAL STRUCTURE; DISKS (STRUCTURAL COMPONENTS); ENZYMES; HYDROGEN; HYDROPHOBICITY; MOLECULES; PHENOLS;

LIGANDS;

ALANINE; ANILINE; BENZENE; ETHYLBENZENE; GLUTAMIC ACID; LEUCINE; LYSOZYME; METHIONINE; PARA XYLENE; PHENOL; PYRIDINE; T4 ENZYME; TOLUENE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENERGY TRANSFER; ENTROPY; ENZYME ENGINEERING; HYDROGEN BOND; HYDROPHOBICITY; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING; MUTATION; NONHUMAN; PKA; PRIORITY JOURNAL; PROTEIN STABILITY; SUBSTITUTION REACTION;

AMINO ACID SUBSTITUTION; BACTERIOPHAGE T4; CRYSTALLOGRAPHY, X-RAY; HYDROPHOBICITY; LIGANDS; MURAMIDASE; MUTAGENESIS, SITE-DIRECTED; PROTEIN FOLDING; PROTEIN STABILITY; STATIC ELECTRICITY; THERMODYNAMICS;

EID: 70349135928     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900685j     Document Type: Article

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