Development of highly sensitive Bicistronic vector based non-radioactive antigen-specific cytotoxicity assay

Journal of Immunological Methods

Volumn 349, Issue 1-2, 2009, Pages 28-37

  Gupta, Pranav ^a   Tayal, Ruchi ^a   Durgapal, Hemlata ^a   Rath, Satyajit ^b   Acharya, Subrat Kumar ^a   Panda, Subrat Kumar ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

^b NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

Bicistronic vector; Cytotoxic T lymphocytes; Cytotoxicity assay; Reporter gene

Indexed keywords

CHROMIUM 51; ENHANCED GREEN FLUORESCENT PROTEIN; FIREFLY LUCIFERASE; HEPATITIS B CORE ANTIGEN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN SPECIFICITY; ARTICLE; CELL MEDIATED CYTOTOXICITY; CELL STRAIN HEPG2; CELL VIABILITY; CONTROLLED STUDY; CYTOLYSIS; CYTOTOXIC T LYMPHOCYTE; EFFECTOR CELL; FEMALE; FIBROBLAST; GENE EXPRESSION; GENETIC TRANSFECTION; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; IN VIVO STUDY; INTERMETHOD COMPARISON; LYMPHOMA CELL LINE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; REPORTER GENE; T LYMPHOCYTE; VIRUS IMMUNITY;

ANIMALS; BALB 3T3 CELLS; CELL LINE, TUMOR; CHROMIUM RADIOISOTOPES; CYTOTOXICITY TESTS, IMMUNOLOGIC; EPITOPES; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GREEN FLUORESCENT PROTEINS; HEPATITIS B CORE ANTIGENS; HEPATITIS B VIRUS; HUMANS; IMMUNIZATION; MICE; MICE, INBRED BALB C; T-LYMPHOCYTES, CYTOTOXIC; TRANSFECTION;

HEPATITIS B VIRUS; MUS;

EID: 70149096841     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.08.001     Document Type: Article

References (36)

1. Bachy M., Bonnin-Rivalland A., Tilliet V., and Trannoy E. Beta galactosidase release as an alternative to chromium release in cytotoxic T-cell assays. J. Immunol. Methods 230 (1999) 37
2. Bertoletti A., Southwood S., Chesnut R., Sette A., Falco M., Ferrara G.B., Penna A., Boni C., Fiaccadori F., and Ferrari C. Molecular features of the hepatitis B virus nucleocapsid T-cell epitope 18-27: interaction with HLA and T-cell receptor. Hepatology 26 (1997) 1027
3. Blomberg K., Granberg C., Hemmila I., and Lovgren T. Europium- labeled target cells in an assay of natural killer cell activity. II. Significance and specificity of the method. J. Immunol. Methods 92 (1986) 117
4. Bruning J.W., Kardol M.J., and Arentzen R. Carboxyfluorescein fluorochromasia assays. I. Non-radioactively labeled cell mediated lympholysis. J. Immunol. Methods 33 (1980) 33
5. Brunner K.T., Mauel J., Cerottini J.C., and Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology 14 (1968) 181
6. Coligan John E. Current protocols in immunology., Vol.1; National Institute of Health, USA.
7. Ewen C.L., Rong J., Kokaji A.I., Bleackley R.C., and Kane K.P. Evaluating antigen-specific cytotoxic T lymphocyte responses by a novel mouse granzyme B ELISPOT assay. J. Immunol. Methods 308 (2006) 156
8. Fischer K., Andreesen R., and Mackensen A. An improved flow cytometric assay for the determination of cytotoxic T lymphocyte activity. J. Immunol. Methods 259 (2002) 159
9. Fujimoto C., Nakagawa Y., Ohara K., and Takahashi H. Polyriboinosinic polyribocytidylic acid [poly(I:C)]/TLR3 signaling allows class I processing of exogenous protein and induction of HIV-specific CD8+ cytotoxic T lymphocytes. Int. Immunol. 16 (2004) 55
10. Fulcher D.A., Lyons A.B., Korn S.L., Cook M.C., Koledafi C., Parish C., de St F., Groth B., and Basten A. The fate of self-reactive B cells depends primarily on the degree of antigen receptor engagement and availability of T cell help. J. Exp. Med. 183 (1996) 2313
11. Geissler M., Tokushige K., Chante C.C., Zurawski Jr. V.R., and Wands J.R. Cellular and humoral immune response to hepatitis B virus structural proteins in mice after DNA-based immunization. Gastroenterology 112 (1997) 1307
12. Glimcher L.H., Kim J.K., Green I., and Paul W.E. Ia antigen-bearing B cell tumor lines can present protein antigen and alloantigen in a major histocompatibility complex-restricted fashion to antigen-reactive T cells. J. Exp. Med. 155 (1982) 445
13. Goldberg J.E., Sherwood S.W., and Clayberger C. A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and two-color flow cytometry. J. Immunol. Methods. 224 (1999) 1
14. Guidotti L.G., Rochford R., Chung J., Shapiro M., Purcell R., and Chisari F.V. Viral clearance without destruction of infected cells during acute HBV infection. Science 284 (1999) 825
15. Hermans I.F., Silk J.D., Yang J., Palmowski M.J., Gileadi U., McCarthy C., Salio M., Ronchese F., and Cerundolo V. The VITAL assay: a versatile fluorometric technique for assessing CTL- and NKT-mediated cytotoxicity against multiple targets in vitro and in vivo. J. Immunol. Methods 285 (2004) 25
16. Hussain R.F., Nouri A.M.E., and Oliver R.T.D. A new approach for measurement of cytotoxicity using colorimetric assay. J. Immunol. Methods 160 (1993) 89
17. Korzeniewski C., and Callewaert D.M. An enzyme-release assay for natural cytotoxicity. J. Immunol. Methods 64 (1983) 313
18. Maini M.K., Boni C., Lee C.K., Larrubia J.R., Reignat S., Ogg G.S., King A.S., Herberg J., Gilson R., Alisa A., Williams R., Vergani D., Naoumov N.V., Ferrari C., and Bertoletti A. The role of virus-specific CD8(+) cells in liver damage and viral control during persistent hepatitis B virus infection. J. Exp. Med. 191 (2000) 1269
19. Maini M.K., Reignat S., Boni C., Ogg G.S., King A.S., Malacarne F., Webster G.J., and Bertoletti A. T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection. Eur. J. Immunol. 30 (2000) 3067
20. Moore M.W., Carbone F.R., and Bevan M.J. Introduction of soluble proteins into the class I pathway of antigen processing and presentation. Cell 54 (1988) 777
21. Mukherjee P., Dani A., Bhatia S., Singh N., Rudensky A.Y., George A., Bal V., Mayor S., and Rath S. Efficient presentation of both cytosolic and endogenous transmembrane protein antigens on MHC class II is dependent on cytoplasmic proteolysis. J. Immunol. 167 (2001) 2632
22. Niu Q.-X., Zhao C.-Y., and Jing A.-Z. An evaluation of the colorimetric assays based on enzymatic reactions used in the measurement of human natural cytotoxicity. J. Immunol. Methods 251 (2001) 11
23. Ozdemir O., Ravindranath Y., and Savasan S. Cell-mediated cytotoxicity evaluation using monoclonal antibody staining for target or effector cells with annexinV/propidium iodide colabeling by fluorosphere-adjusted counts on three-color flow cytometry. Cytometry A. 56 (2003) 53
24. Rahman F., Dahmen A., Herzog-Hauff S., Bocher W.O., Galle P.R., and Lohr H.F. Cellular and humoral immune responses induced by intradermal or intramuscular vaccination with the major hepatitis B surface antigen. Hepatology. 31 (2000) 521
25. Rodriguez F., Zhang J., and Whitton J.L. DNA immunization: ubiquitination of a viral protein enhances cytotoxic T lymphocyte induction and antiviral protection but abrogates antibody induction. J. Virol. 71 (1997) 8497
26. Russell J.H., and Ley T.J. Lymphocyte-mediated cytotoxicity. Annu. Rev. Immunol. 20 (2002) 323
27. Schafer H., Schafer A., Kiderlen A.F., Masihi K.N., and Burger R. A highly sensitive cytotoxicity assay based on the release of reporter enzymes, from stably transfected cell lines. J. Immunol. Methods 204 (1997) 89
28. + T cell responses in peripheral blood and mucosal tissues. Clin Exp. Immunol. 130 (2002) 172
29. Shafer-Weaver K., Sayers T., Strobl S., Derby E., Ulderich T., Baseler M., and Malyguine A. The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity. J. Transl. Med. 29 (2003) 14
30. Sheehy M.E., McDermott A.B., Furlan S.N., Klenerman P., and Nixon D.F. A novel technique for the fluorometric assessment of T lymphocyte antigen specific lysis. J. Immunol. Methods 249 (2001) 99
31. Szekeres J., Pacsa A.S., and Pejtsik B. Measurement of lymphocyte cytotoxicity by assessing endogenous alkaline phosphatase activity of the target cells. J. Immunol. Methods 40 (1981) 151
32. Tomaru U., Yamano Y., Nagai M., Maric D., Kaumaya P.T., Biddison W., and Jacobson S. Detection of virus-specific T cells and CD8+ T-cell epitopes by acquisition of peptide-HLA-GFP complexes: analysis of T-cell phenotype and function in chronic viral infections. Nat. Med. 9 (2003) 469
33. 51Cr-labeled targets for natural killer cytotoxicity assay. J. Immunol. Methods 122 (1989) 15
34. Wood K.V. Recent advances and prospects for use of beetle luciferase as genetic reporters. In: Stanley P., and Kricka L. (Eds). Bioluminescence and Chemiluminescence: Current Status (1991), John Wiley and Sons, Chichester 11
35. Xing Y.P., Huang Z.H., Wang S.X., Cai J., Li J., Chou T.H., and Lu S. Novel DNA vaccine based on hepatitis B virus core gene induces specific immune responses in BALB/c mice. World J. Gastroenterol. 11 (2005) 4583
36. Zhu M., Xu X., Liu H., Liu X., Wang S., Dong F., Yang B., and Song G. Enhancement of DNA vaccine potency against herpes simplex virus 1 by co-administration of an interleukin-18 expression plasmid as a genetic adjuvant. J. Med. Microbiol. 52 (2003) 223