Cloning, expression, and characterization of a peculiar choline-binding β-galactosidase from Streptococcus mitis

Applied and Environmental Microbiology

Volumn 75, Issue 18, 2009, Pages 5972-5980

  Campuzano, Susana ^a   Serra, Beatriz ^a,b   Llull, Daniel ^a   García, José L ^a   Garcí, Pedro ^a  

^a INSTITUTO DE SALUD CARLOS III   (Spain)

^b UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; CELL MEMBRANES; CLONING; ENCODING (SYMBOLS); ENZYME ACTIVITY; ESCHERICHIA COLI; GENE ENCODING; PURIFICATION;

BINDING CAPACITIES; BIOTECHNOLOGICAL APPLICATIONS; CHOLINE BINDING DOMAIN; CULTURE MEDIUM; FUSION PROTEINS; MODULAR STRUCTURES; OPTIMUM TEMPERATURE; SPECIFIC ACTIVITY;

RECOMBINANT PROTEINS;

BETA GALACTOSIDASE; CHOLINE; DNA FRAGMENT; GENOMIC DNA; GLUCOSE; HYBRID PROTEIN; POLYHISTIDINE TAG;

BACTERIUM; CLONE; ENZYME ACTIVITY; GENE EXPRESSION;

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL STRAIN; CELL SURFACE; CELL WALL; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE IDENTIFICATION; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; PH; PROTEIN PURIFICATION; SMT1224 GENE; STREPTOCOCCUS MITIS;

ESCHERICHIA COLI; STREPTOCOCCUS MITIS;

EID: 70149095026     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00618-09     Document Type: Article

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