The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture

Biomaterials

Volumn 30, Issue 31, 2009, Pages 6265-6275

  Chang, Jui Chih ^a   Hsu, Shan hui ^b   Chen, David C ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Adipose derived adult stem cells; Alginate; Chondrogenesis; Fibronectin; RGD chimeric protein; RhoA activity

Indexed keywords

ADIPOSE-DERIVED ADULT STEM CELLS; CHONDROGENESIS; FIBRONECTIN; RGD-CHIMERIC PROTEIN; RHOA ACTIVITY;

ALGINATE; AMINES; BIOACTIVITY; CARTILAGE; CELL MEMBRANES; COLLAGEN; GENE EXPRESSION; THREE DIMENSIONAL;

CELL CULTURE;

AGGRECAN; ALGINIC ACID; ALPHA5 INTEGRIN; ARGINYLGLYCYLASPARTIC ACID; BETA1 INTEGRIN; CELLULOSE; CHIMERIC PROTEIN; COLLAGEN TYPE 10; COLLAGEN TYPE 3; FIBRONECTIN; FLUORESCEIN ISOTHIOCYANATE; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; TRANSCRIPTION FACTOR SOX9; TRANSFORMING GROWTH FACTOR BETA3;

ADIPOSE TISSUE; ADULT STEM CELL; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CHONDROGENESIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE TECHNIQUE; DIFFUSION; ENZYME INHIBITION; FEASIBILITY STUDY; GENE EXPRESSION; GENE EXPRESSION REGULATION; ISOELECTRIC POINT; MESENCHYMAL STEM CELL; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN INDUCTION; PROTEIN MODIFICATION; PROTEIN TRANSPORT; RAT; SIGNAL TRANSDUCTION;

ALGINATES; ANIMALS; BLOTTING, WESTERN; CHONDROGENESIS; COATED MATERIALS, BIOCOMPATIBLE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; GLUCURONIC ACID; HEXURONIC ACIDS; MESENCHYMAL STEM CELLS; OLIGOPEPTIDES; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TISSUE ENGINEERING;

EID: 69649097014     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2009.07.064     Document Type: Article

References (41)

1. Mauck R.L., Byers B.A., Yuan X., and Tuan R.S. Regulation of cartilaginous ECM gene transcription by chondrocytes and MSCs in 3D culture in response to dynamic loading. Biomech Model Mechanobiol 6 1-2 (2007) 113-125
2. Meinel L., Hofmann S., Karageorgiou V., Zichner L., Langer R., Kaplan D., et al. Engineering cartilage-like tissue using human mesenchymal stem cells and silk protein scaffolds. Biotechnol Bioeng 88 3 (2004) 379-391
3. Hwang N.S., Varghese S., Zhang Z., and Elisseeff J. Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels. Tissue Eng 12 9 (2006) 2695-2706
4. Yamaoka H., Asato H., Ogasawara T., Nishizawa S., Takahashi T., Nakatsuka T., et al. Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. J Biomed Mater Res A 78 1 (2006) 1-11
5. Salinas C.N., and Anseth K.S. The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities. Biomaterials 29 15 (2008) 2370-2377
6. Connelly J.T., Garcia A.J., and Levenston M.E. Inhibition of in vitro chondrogenesis in RGD-modified three-dimensional alginate gels. Biomaterials 28 6 (2007) 1071-1083
7. Chen DC, Hu NT, Chen YJ, Hseu TH. Chimeric proteins with a cellulose binding domain. [US patent 6407208B1]; 2002: 6-18.
8. Shoseyov O., and Warren R.A.J. Cellulose binding domains - a novel fusion technology for efficient, low cost purification and immobilization of recombinant proteins. Innovations 7 (1997) 1-3
9. Hsu S.H., Chu W.P., Lin Y.S., Chiang Y.L., Chen D.C., and Tsai C.L. The effect of an RGD-containing fusion protein CBD-RGD in promoting cellular adhesion. J Biotechnol 111 2 (2004) 143-154
10. Hsu S.H., Chuang S.C., Chen C.H., and Chen D.C. Endothelial cell attachment to the gamma irradiated small diameter polyurethane vascular grafts. Biomed Mater Eng 16 6 (2006) 397-404
11. Hsu S.H., Sun S.H., and Chen D.C. Improved retention of endothelial cells seeded on polyurethane small-diameter vascular grafts modified by a recombinant RGD-containing protein. Artif Organs 27 12 (2003) 1068-1078
12. Hsu S.H., Whu S.W., Hsieh S.C., Tsai C.L., Chen D.C., and Tan T.S. Evaluation of chitosan-alginate-hyaluronate complexes modified by an RGD-containing protein as tissue-engineering scaffolds for cartilage regeneration. Artif Organs 28 8 (2004) 693-703
13. Tavella S., Bellese G., Castagnola P., Martin I., Piccini D., Doliana R., et al. Regulated expression of fibronectin, laminin and related integrin receptors during the early chondrocyte differentiation. J Cell Sci 110 Pt 18 (1997) 2261-2270
14. DeLise A.M., Fischer L., and Tuan R.S. Cellular interactions and signaling in cartilage development. Osteoarthr Cartil 8 5 (2000) 309-334
15. Cali G., Mazzarella C., Chiacchio M., Negri R., Retta S.F., Zannini M., et al. RhoA activity is required for fibronectin assembly and counteracts beta 1B integrin inhibitory effect in FRT epithelial cells. J Cell Sci 112 6 (1999) 957-965
16. Defilippi P., Venturino M., Gulino D., Duperray A., Boquet P., Fiorentini C., et al. Dissection of pathways implicated in integrin-mediated actin cytoskeleton assembly - involvement of protein kinase C, Rho GTPase, and tyrosine phosphorylation. J Biol Chem 272 35 (1997) 21726-21734
17. Parsons J.T. Integrin-mediated signalling: regulation by protein tyrosine kinases and small GTP-binding proteins. Curr Opin Cell Biol 8 2 (1996) 146-152
18. Ridley A.J., and Hall A. The small Gtp-binding protein Rho regulates the assembly of focal adhesions and actin stress fibers in response to growth-factors. Cell 70 3 (1992) 389-399
19. Lee J., Ko M., and Joo C.K. Rho plays a key role in TGF-beta1-induced cytoskeletal rearrangement in human retinal pigment epithelium. J Cell Physiol 216 2 (2008) 520-526
20. Woods A., Wang G., and Beier F. RhoA/ROCK signaling regulates Sox9 expression and actin organization during chondrogenesis. J Biol Chem 280 12 (2005) 11626-11634
21. Woods A., and Beier F. RhoA/ROCK signaling regulates chondrogenesis in a context-dependent manner. J Biol Chem 281 19 (2006) 13134-13140
22. Wee S., and Gombotz W.R. Protein release from alginate matrices. Adv Drug Deliv Rev 31 3 (1998) 267-285
23. Smidsrod O., and Skjak-Braek G. Alginate as immobilization matrix for cells. Trends Biotechnol 8 3 (1990) 71-78
24. Zuk P.A., Zhu M., Ashjian P., De Ugarte D.A., Huang J.I., Mizuno H., et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13 12 (2002) 4279-4295
25. Liu C.Z., Wang Y.W., Shen M.C., and Huang T.F. Analysis of human platelet glycoprotein IIb-IIIa by fluorescein isothiocyanate-conjugated disintegrins with flow cytometry. Thromb Haemost 72 6 (1994) 919-925
26. Wilkins M.R., Gasteiger E., Bairoch A., Sanchez J.C., Williams K.L., Appel R.D., et al. Protein identification and analysis tools in the ExPASy server. Methods Mol Biol 112 (1999) 531-552
27. Mardilovich A., Craig J.A., McCammon M.Q., Garg A., and Kokkoli E. Design of a novel fibronectin-mimetic peptide-amphiphile for functionalized biomaterials. Langmuir 22 7 (2006) 3259-3264
28. Garcia A.J., Schwarzbauer J.E., and Boettiger D. Distinct activation states of alpha5beta1 integrin show differential binding to RGD and synergy domains of fibronectin. Biochemistry 41 29 (2002) 9063-9069
29. Leahy D.J., Aukhil I., and Erickson H.P. 2.0 a crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region. Cell 84 1 (1996) 155-164
30. Dillo A.K., Ochsenhirt S.E., McCarthy J.B., Fields G.B., and Tirrell M. Adhesion of alpha5beta1 receptors to biomimetic substrates constructed from peptide amphiphiles. Biomaterials 22 12 (2001) 1493-1505
31. Connelly J.T., Garcia A.J., and Levenston M.E. Interactions between integrin ligand density and cytoskeletal integrity regulate BMSC chondrogenesis. J Cell Physiol 217 1 (2008) 145-154
32. Puolakkainen P.A., Ranchalis J.E., Gombotz W.R., Hoffman A.S., Mumper R.J., and Twardzik D.R. Novel delivery system for inducing quiescence in intestinal stem cells in rats by transforming growth factor beta 1. Gastroenterology 107 5 (1994) 1319-1326
33. Segi N., Yotsuyangi T., and Ikeda K. Interaction of calcium-induced alginate beads with propranolol. Chem Pharm Bull 37 (1989) 3092-3095
34. Sawhney A.S., and Hubbell J.A. Poly(ethylene oxide)-graft-poly(l-lysine) copolymers to enhance the biocompatibility of poly(l-lysine)-alginate microcapsule membranes. Biomaterials 13 12 (1992) 863-870
35. Petrie T.A., Capadona J.R., Reyes C.D., and Garcia A.J. Integrin specificity and enhanced cellular activities associated with surfaces presenting a recombinant fibronectin fragment compared to RGD supports. Biomaterials 27 31 (2006) 5459-5470
36. Keselowsky B.G., Collard D.M., and Garcia A.J. Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation. Proc Natl Acad Sci U S A 102 17 (2005) 5953-5957
37. Watanabe H., de Caestecker M.P., and Yamada Y. Transcriptional cross-talk between Smad, ERK1/2, and p38 mitogen-activated protein kinase pathways regulates transforming growth factor-beta-induced aggrecan gene expression in chondrogenic ATDC5 cells. J Biol Chem 276 17 (2001) 14466-14473
38. Drissi M.H., Li X., Sheu T.J., Zuscik M.J., Schwarz E.M., Puzas J.E., et al. Runx2/Cbfa1 stimulation by retinoic acid is potentiated by BMP2 signaling through interaction with Smad1 on the collagen X promoter in chondrocytes. J Cell Biochem 90 6 (2003) 1287-1298
39. Han F., Gilbert J.R., Harrison G., Adams C.S., Freeman T., Tao Z., et al. Transforming growth factor-beta1 regulates fibronectin isoform expression and splicing factor SRp40 expression during ATDC5 chondrogenic maturation. Exp Cell Res 313 8 (2007) 1518-1532
40. Song J.J., Aswad R., Kanaan R.A., Rico M.C., Owen T.A., Barbe M.F., et al. Connective tissue growth factor (CTGF) acts as a downstream mediator of TGF-beta1 to induce mesenchymal cell condensation. J Cell Physiol 210 2 (2007) 398-410
41. Lu Z.F., Zandieh D.B., Huang C.L., Bank R.A., and Helder M.N. Beta1 integrins regulate chondrogenesis and rock signaling in adipose stem cells. Biochem Biophys Res Commun 372 4 (2008) 547-552