Engineering new aptamer geometries for electrochemical aptamer-based sensors

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 7321, Issue , 2009, Pages

  White, Ryan J ^a   Plaxco, Kevin W ^a,b  

^a Department of Chemistry and Biochemistry ^*

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Aptamers; Cocaine; Electrochemical detection; Optimization; Small molecule detection; Whole blood

Indexed keywords

APTAMERS; COCAINE; ELECTROCHEMICAL DETECTION; SMALL MOLECULE DETECTION; WHOLE BLOOD;

ARCHITECTURAL DESIGN; BLOOD; ELECTRODES; MOLECULES; OPTIMIZATION; SIGNAL DETECTION; SIGNAL TRANSDUCTION;

SENSORS;

EID: 69649086715     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.820419     Document Type: Conference Paper

References (21)

1. Zuo, X., Song, S., Zhang, J., Pan, D., Wang, L., and Fan, C., "A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP, " J. Am. Chem. Soc., 129, 1042-1043 (2007).
2. Baker, B.R., Lai, R.Y., Wood, M.S., Doctor, E.H., Heeger, A.J., and Plaxco, K. W., "An electronic, aptamer-based small-molecule sensor for the rapid, label-free detection of cocaine in adulterated samples and biological fluids, " J. Am. Chem. Soc., 128, 3138-3139 (2006).
3. White, R. J., Phares, N., Lubin, A. A., Xiao, Y., and Plaxco, K. W., "Optimization of electrochemical aptamer-based sensors via optimization of probe packing density and surface chemistry, " Langmuir, 24, 10513-10518 (2008).
4. Swensen, J. S., Xiao, Y., Ferguson, B. S., Lubin, A. A., Lai, R.Y., Heeger, A. J., Plaxco, K. W., and Soh, H. T., "Continuous, realtime monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor, " J. Am. Chem. Soc., ASAP Article (2009).
5. Ferapontova, E. E., Olsen, E. M., and Gothelf, K. V., "An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum, " J. Am. Chem. Soc., 130, 4256-4258 (2008).
6. Xiao, Y., Rowe, A. A., and Plaxco, K. W., "Electrochemical detection of parts-per-billion lead via an electrode-bound DNAzyme assembly, " J. Am. Chem. Soc., 129, 262-263 (2007).
7. Radi, A., and O'Sullivan, C. K., "Aptamer conformational switch as sensitive electrochemical biosensor for potassium ion recognition, " Chem. Commun., 32, 3432-3434 (2006).
8. Lai, R.Y., Plaxco, K. W., and Heeger, A. J., "Aptamer-based electrochemical detection of picomolar platelet-derived growth factor directly in blood derum, " Anal. Chem., 79, 229-233 (2007).
9. Xiao, Y., Piorek, B. D., Plaxco, K. W., and Heeger, A. J., "A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement, " J. Am. Chem. Soc. 127, 17990-17991 (2005).
10. Xiao, Y., Uzawa, T., White, R. J., DeMartini, D., and Plaxco, K.W., "On the signaling of electrochemical, aptamer-based sensors: collision- and folding-based mechanisms, " Electroanalysis, In press (2009).
11. Stojanovic, M. N., de Prada, P., and Landry, D.W., "Aptamer-based folding fluorescent sensor for cocaine, " J. Am. Chem. Soc., 123, 4928-4931 (2001).
12. Xiao, Y., Lai, R. Y., and Plaxco, K. W., "Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing, " Nat. Protocols., 2, 2875-2880 (2007).
13. Herne, T. M., and Tarlov, M.J., "Characterization of DNA probes immobilized on gold surfaces, " J. Am. Chem. Soc., 119, 8916-8920 (1997).
14. Ricci, F., Lai, R. Y., Heeger, A. J., Plaxco, K. W., and Sumner, J. J., "Effect of molecular crowding on the response of an electrochemical DNA sensor, " Langmuir, 23, 6827-6834 (2007).
15. Bard A. J. and Faulkner, L. R., [Electrochemical Methods: Fundamentals and Applications], 2nd ed., Wiley, New York (2001).
16. Bozic, R. G., West, A. C., and Levicky, R., "Square wave voltammetric detection of 2, 4, 6-trinitrotoluene and 2, 4-dinitrotoluene on a gold electrode modified with self-assembled monolayers, " Sensors and Actuators B, 133, 509-515 (2008).
17. Meller, A., Nivon, L., and Branton, D., "Voltage-driven DNA translocations through a nanopore, " Phys. Rev. Lett., 86, 3435-3438 (2001).
18. Zuo, X., Xiao, Y., and Plaxco, K. W., "High specificity, electrochemical sandwich assays based on single aptamer sequences and suitable for the direct detection of small-molecule targets in blood and other complex matrices, " J. Am. Chem. Soc., Submitted (2009).
19. Uzawa, T., Cheng, R. R., Cash, K. J., Makarov, D. E., and Plaxco, K. W., "Characterization of the length and viscosity dependence of the end-to-end collision dynamics of single-stranded DNA, " Biophys. J., Submitted (2009).
20. Wallace, M. I., Ying, L., Balasubramanian, S., and Klenerman, D., "Non-Arrhenius kinetics for the loop closure of a DNA hairpin, " Proc. Natl. Acad. Sci., 98, 5584-5589 (2001).
21. Lowe, G., "Blood rheology in vitro and in vivo, " Baillière's Clinical Haematology, 1, 597-636 (1987).