Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry

Acta Oceanologica Sinica

Volumn 28, Issue 2, 2009, Pages 103-114

  Jianjun, Hou ^a,b,c,d,e   Hongyan, Lai ^b,d   Bangqin, Huang ^c   Jixin, Chen ^c  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b HUBEI NORMAL UNIVERSITY   (China)

^c XIAMEN UNIVERSITY   (China)

^d Yangtze River Fish Inst Jingzhou   (China)

^e INSTITUTE OF HYDROBIOLOGY   (China)

Author keywords

DNA probes; Flow cytometry; Fluorescence in situ hybridization; Oligonucleotide; Prorocentrum minimum; Takayama pulchella

Indexed keywords

ALGAL COMMUNITY; CLONE; DINOFLAGELLATE; DNA; FLOW CYTOMETRY; FLUORESCENCE; GENETIC ANALYSIS; HYBRIDIZATION; IN SITU TEST; MICROSCOPY; SAMPLING;

ALGAE; PROROCENTRUM MINIMUM;

EID: 69549111581     PISSN: 0253505X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Amann R. 1995. In situ identification of microorganisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In: Akkermann A D L, VanElsas J D, de-Bruijin F J, eds. Molecular Microbial Ecology Manual. Dordrecht. Dordrecht: Kluwer Academic Publishers, 1-15
2. Anderson D M, Kulis D M, Keafer B A. 1999. Detection of the toxic dinoflagellate Alexandrium fundyense (Dinophyceae) with oligonucleotide and antibody probes: variability in labeling intensity with physiological condition. J Phycol, 35: 870-883
3. Biegala I C, Kennaway G, Alverca E, et al. 2002. Identification of bacteria associated with dinoflagellates (Dinophyceae) Alexandrium spp. using tyramide signal amplification-fluorescent in situ hybridization and confocal microscopy. J Phycol, 38: 404-411
4. Chao Min, Zhang Lihua, Zhang Jing. 2005. Picoplankton measurement with flow cytometry: protocol of fixation and preservation. Chinese Journal of Applied and Environmental Biology, 11(4): 448-452
5. Chen Jixin, Huang Bangqin, Jiao Nianzhi, et al. 2003. Fluorescent in situ hybridization with oligonucleotide probes to identify Alexandrium tamarense and genus Alexandrium. In: Ho KC, Lu SH, Yu TS, eds. Recent Advances in the Prevention and Management of Harmful Algal Blooms in the South China Sea. Proceedings of the International Conference on the Prevention and Management of Harmful Algal Blooms in the South China Sea (HABSCS2003). Hong Kong: the Croucher Foundation, 81-88
6. Connell L, Ray J, Litaker W, et al. 2006. Enhanced detection levels in a semi-automated sandwich hybridisation assay using a peptide nucleic acid (PNA) signal probe. Afr J Mar Sci, 28: 237-239
7. Denardou-Queneherve A, Grzebyk D, Pouchus Y F, et al. 1999. Toxicity of French strains of the dinoflagellate Prorocentrum minimum experimental and natural contaminations of mussels. Toxicon, 37: 1711-1719
8. de Salas M F, Bolch C J S, Botes L. 2003. Takayama Gen Nov (Gymnodiniales, Dinophyceae). A new genus of unarmored dinoflagellates with sigmoid apical grooves, including the description of two new species. J Phycol, 39: 1233-1246
9. Gu Haifeng, Sun Jun, Zou Yinglin, et al. 2006. Morphology, growth and molecular character of Takayama pulchella from the Xiamen Bay. Acta Ecologica Sinica (in Chinese), 26: 1021-1027
10. Guo Feng, Lin Junmin, Huang Lingfeng, et al. 2002. Ecological characteristics of phytoplankton in shrimp cultivation area from Pantu, Xiamen. J Oceanogra Taiwan Strait (in Chinese), 21: 469-482
11. Hosoi-Tanabe S, Sako Y. 2005. Rapid detection of natural cells of Alexandrium tamarense and A. catenella (Dinophyceae) by fluorescence in situ hybridization. Harmful Algae, 4: 319-328
12. Hosoi-Tanabe S, Sako Y. 2006. Development and application of fluorescence in situ hybridization (FISH) method for simple and rapid identification of the toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella in cultured and natural sea-water. Fish Sci, 72: 77-82
13. Hou Jianjun, Huang Hui, Lei Hongling, et al. 2007. Observation of harmful algal blooms caused by Prorocentrum and Gymnodinium species in the western Xiamen Harbour. J Fish Sci Chin (in Chinese), 14: 950-960
14. Huang Bangqin, Hou Jianjun, Lin Senjie, et al. 2008. Development of a PNA probe for the detection of the toxic dinoflagellate Takayama pulchella. Harmful Algae, 7: 495-503
15. Jiao Nianzhi, Yang Yinjie, Luo Tingwei. 2004. Membrane potential based characterization by flow cytometry of physiological states in an aerobic anoxygenic phototrophic bacterium. Aquatic Microbial Ecology, 37(2): 149-158
16. Lam P, Cowen J P, 2004. Processing deep-sea particle-rich water samples for fluorescence in situ hybridization: consideration of storage effects, preservation, and sonication. Appl Environ Microbiol, 70: 25-33
17. Larsen J. 1994. Unarmoured dinoflagellates from Australian waters: I. The genus Gymnodinium (Gymnodiniales, Dinophyceae). Phycologia, 33: 342-349
18. Lee S, Kemp P F. 1994. Single-cell RNA content of natural marine planktonic bacteria measured by hybridization with multiple 16S rRNA-targeted fluorescent probes. Limnol Oceanogr, 39: 869-879
19. Lin Jinmei. 1990. Distribution of planktonic Pyrrophyta in the western waters around Xiamen Island. Acta Ecol Sin (in Chinese), 10(2): 139-144
20. Lin Yongshui, Zhou Jinming, He Jianzong. 2001. Harmful Algae (in Chinese). Beijing: Science Press, 6-9
21. Martin L, Laure G,Vaulot D. 1996. Identification of the class Prymnesiophyceae and the genus Phaeocystis with ribosomal RNA-targeted nucleic acid probes detected by flow cytometry. J Phycol, 32: 858-868
22. Moter A, Gobel U B. 2000. Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. J Microbiol Methods, 41: 85-112
23. Nakazima M. 1968. Studies on the source of shellfish poison in Lake Hamana - IV. Identification and collection of the noxious dinoflagellate. Bull Jpn Soc Sci Fish, 34: 130-132
24. Onoue K, Nozawa K, Kumanda K. 1985. Occurrence of a toxic dinoflagellate, "Gymnodinium type 84K" in Kagoshima Bay. Bull Jpn Soc Sci Fish, 51: 1567-1579
25. Rhodes L L, Haywood A J, Adamson J et al. 2004. DNA probes for the detection of Karenia species in New Zealands coastal waters. In: Steidinger K, Landsberg J, Vargo G, eds. Harmful Algae, 2002. Florida Fish and Wildlife Cons Comm, Florida Inst Oceanography and IOC of UNESCO, 273-275
26. Rhodora V A, Yasuwo F, Leni G Y, et al. 2005. Prorocentrum minimum bloom and its possible link to a massive fish kill in Bolinao, Pangasinan, Northern Philippines. Harmful Algae, 4: 519-524
27. Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 213-222
28. Scholin C A, Buck K R, Britschgi T, et al. 1996. Identification of Pseudo-nizschia australis (Bacillariophyceae) using rRNA-targeted probes in whole cell and sandwich hybridization formats. Phycologia, 35: 190-197
29. Schonhuber W, Puchs B, Juretschko S, et al. 1997. Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Appl Environ Microbiol, 63: 3268-3273
30. Shao Peng, Chen Yueqin, Zhou Hui, et al. 2004. Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis. Mar Biol, 144: 215-224
31. Silva E S, Sousa I. 1981. Experimental work on the di-noflagellate toxin production. Arquivos do Institute Nacional de Saude, VI. Lisbon: Escola Nacional Publica, 381-387
32. Simon N, Brenner J, Edvardsen B. 1997. The identification of Chrysochromulina and Prymnesium species (Haptophyta, Prymnesiophyceae) using fluorescent or chemiluminescent oligonucleotide probes: a means of improving studies on toxic algae. Eur J Phycol, 32: 393-401
33. Usup G, Pin L C, Ahmad A et al. 2002. Phylogenetic relationship of Alexandrium tamiyavanichii (Dinophyceae) to other Alexandrium species based on ribosomal RNA gene sequences. Harmful Algae, 1: 59-68
34. Worden A Z, Binder B J. 2003. Growth regulation of rRNA content in Prochlorococcus and Synechococcus (marine cyanobacteria) measured by whole-cell hybridization of rRNA-targeted peptide nucleic acids. J Phycol, 39: 527-534
35. Zhang Shuijin, Xu Kuncan, Chen Qihuan, et al. 1988. Observation of a red tide event in the western Xiamen Bay. Acta Oceanologica Sinica (in Chinese), 10: 602-608
36. Zhou Mingjiang, Zhu Mingyun, Zhang Jing. 2001. Status of harmful algal blooms and related research activities in China. Chin Bull of Life Sci (in Chinese), 13(2): 54-59