Purification of three major forms of β-hCG from urine and production of polyclonal antibodies against them

Clinical Biochemistry

Volumn 42, Issue 13-14, 2009, Pages 1476-1482

  Khademi, Fatemeh ^a   Hamzehee, Koorosh ^b   Mostafaie, Ali ^b   Hajihossaini, Reza ^a  

^a PAYAME NOOR UNIVERSITY   (Iran)

^b KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

hCG; Antibody; Chromatography; Purification; Trypsin inhibitor; Urine

Indexed keywords

CHORIONIC GONADOTROPIN ANTIBODY; CHORIONIC GONADOTROPIN BETA SUBUNIT; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC KIT; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GEL ELECTROPHORESIS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; PREGNANT WOMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; ULTRAFILTRATION; URINALYSIS;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, DEAE-CELLULOSE; CHROMATOGRAPHY, ION EXCHANGE; CONCANAVALIN A; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; FEMALE; HUMANS; IMMUNIZATION; PREGNANCY; RATS; SEPHAROSE;

EID: 69249222634     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2009.05.019     Document Type: Article

References (33)

1. Stenman U.H., Tiitinen A., Alfthan H., and Valmu L. The classification, functions and clinical use of different isoforms of hCG. Hum. Reprod. Update 12 (2006) 769-784
2. Marci J.N., Kasturi R.V., Krantz D.A., et al. Maternal serum Down syndrome screening: free beta protein is a more effective marker than human chorionic gonadotropin. AM. J. Obstet. Gynecol. 163 (1990) 1248-1253
3. Hsu C.D., Chung Y.K., Kardana A., et al. Elevated serum hCG, free β and β-core fragment hCG in severe preeclampsia (1996), Abstract presented at Soc Gyn Invest, Philadelphia
4. Cole L.A. New perspectives in measuring human chorionic gonadotropin levels for measuring and monitoring trophoblast disease. J. Reprod. Med. 39 (1994) 193-200
5. Canick J.A., Kellner L.H., Saller Jr. D.N., Palomaki G.E., Walker R.P., and Osthanondh R. Second trimester levels of maternal urinary gonadotropin peptid in Down syndrome pregnancy. Prenat. Diagn. 15 (1995) 752-759
6. Cuckle H.S., Lles R.K., and Chard T. Urinary β-core fragment: a new approach to Down's syndrome screening. Prenat. Diagn. 14 (1994) 953-958
7. Stenman U.H., Unkila-Kallio L., Korhonen J., and Alfthan H. Immunoprocedures for detecting human chorionic gonadotropin: clinical aspects and doping control. Clin. Chem. 43 (1997) 1293-1298
8. Pfeffer U., Bisacchi D., Morini M., et al. Human chorionic gonadotropin inhibits Kaposi's sarcoma associated angiogenesis, matrix metalloprotease activity, and tumor growth. Endocrinology 143 (2002) 3114-3121
9. Cole L.A., Kardana A., Park S.Y., and Braunstein G.D. The deactivation of hCG by nicking and dissociation. J. Clin. Endocrinol. Metab. 76 (1993) 704-713
10. Fein J.L., Rosen S.W., and Weintraub B.D. Increased glycosylation of serum human chorionic gonadotropin and subunits from eutopic and ectopic sources: comparison with urinary and placental forms. J. Clin. Endocrinol. Metab. 50 (1980) 1111-1120
11. Hoermann R., Spoettl G., Moncayo R., and Mann K. Evidence for the presence of human chorionic gonadotropin (hCG) and free beta subunit of hCG in the human pituitary. J. Clin. Endocrinol. Metab. 179 (1990) 71-86
12. Bock J.L. HCG assays. A plea for uniformity. Am. J. Clin. Pathol. 93 (1990) 432-433
13. Cole L.A., and Kardana A. Discordant results in human chorionic gonadotropin assays. Clin. Chem. 38 (1992) 263-270
14. Cole L.A., Sutton J.M., Higgins T.N., and Cembrowski G.S. Between method variation in human chorionic gonadotropin test results. Clin. Chem. 50 (2004) 874-882
15. Cole L.A., Shahabi S., Butler S.A., et al. Utility of commonly used commercial human chorionic gonadotropin immunoassays in the diagnosis and management of trophoblastic diseases. Clin. Chem. 47 (2001) 308-315
16. Stenman U.H. Standardization of assays for human chorionic gonadotropin. Clin. Chem. 50 (2004) 798-800
17. Got R., and Bourrillon R. Nouvelle methode de purification de Ia gonadotropine choriale humaine. Biochem. Biophys. Acta 42 (1960) 505-512
18. Reisfeld R.A., and Hertz R. Purification of chorionic gonadotropin from the urine of patients with trophoblastic tumors. Biochem. Biophys. Acta 43 (1960) 540-543
19. Van Hell H., Goverde B.C., Schuars A.H., de Jager E., Matthijsen R., and Homan J.D. Purification, characterization and immunochemical properties of human chorionic gonadotropin. Nature 212 (1966) 261-262
20. Choy Y.M., Lau K.M., and Lee C.Y. Purification and characterization of urinary choriogoandotropin from patients with Hydatiform Mole. J. Biol. Chem. 254 (1979) 1159-1163
21. Blith D.L., Akar A.H., Wehmann R.E., and Nisula B.C. Purification of β-core fragment from pregnancy urine and demonstration that it's carbohydrate moieties differ from those of native human chorionic gonadotropin-β. Endocrinology 122 (1988) 173-180
22. Sakakibara R., Miyazaki S., Shigemura T., Tominaga N., Sakai A., and Ishiguro M. Effective purification of human chorionic gonadotropin and its subunits from pregnant women's urinary peptides absorbed on reverse-phase resin. Chem. Pharm. Bull. 38 (1990) 1414-1416
23. Birken S., Chen Y., Gawinowicz M.A., et al. Separation of nicked human chorionic gonadotropin (hCG), intact hCG, and hCG β fragment from standard reference preparations and raw urine samples. Endocrinology 133 (1993) 1390-1397
24. Xu B., Zhu Z., Tang Y., Huang O., and Wang X. Purification of human chorionic gonadotropin from urine by membrane filtration affinity chromatography with a positively charged membrane. Protein Expr. Purif. 16 (1999) 221-223
25. Birken S., Maydelman Y., and Gawinowicz M.A. Preparation and analysis of the common urinary forms of human chorionic gonadotropin. Methods 21 (2000) 3-14
26. Rajabi O., Varasteh A.R., Houssaindokht M.R., and Fazli Bazzaz B.S. Isolation and purification of hCG from the urine of pregnant women. Iran J. Med. Sci. 4 (2002) 8-14
27. 4. Nature 227 (1970) 680-685
28. Hanly W.C. Explain briefly the function of adjuvants; guidelines for use of adjuvants, particularly Freund's adjuvants. AWIC 37 (1995) 127
29. Vierling R.A., and Nesbit L.A. An anti-soybean peroxidase monoclonal antibody conjugated to soybean peroxidase (SPAP) improves immunoassay technology, Plant peroxidase. News Letter 15 (2000) 59-67
30. Bradford M.M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
31. Roig J., Krause J.M., Berger P., and Wolfgang E. Time-dependent folding of immunological epitopes of the human chorionic gonadotropin β-subunit. Mol. Cell. Endocrinol. 260 (2007) 12-22
32. Blith D.L., Wehman R.E., and Nisula B.C. Beta-core chemical and clinical properties. Trends Endocrinol. Metab. 1 (1990) 394-398
33. Udagawa A., Okamato T., Nomura S., Matsuo K., Suzuki H., and Mizutani S. Human chorionic gonadotropin beta-core fragment is present in the human placenta. Mol. Cell. Endocrinol. 139 (1998) 171-178