Interaction of phase variation, host and pressure/gas composition: Pneumococcal gene expression of PsaA, SpxB, Ply and LytA in simulated middle ear environments

International Journal of Pediatric Otorhinolaryngology

Volumn 73, Issue 10, 2009, Pages 1417-1422

  Li Korotky, Ha Sheng ^a,b,c   Lo, Chia Yee ^a   Zeng, Fan Rui ^a   Lo, David ^a   Banks, Juliane M ^a  

^a CHILDREN S HOSPITAL OF PITTSBURGH   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Human middle ear epithelial cell line; Otitis media; Phase variation; Simulated middle ear environment; Streptococcus pneumoniae; Virulence expression

Indexed keywords

AUTOLYSIN; BACTERIAL RNA; PNEUMOLYSIN; PROTEIN PSAA; PYRUVATE OXIDASE; UNCLASSIFIED DRUG;

ARTICLE; AUDITORY TUBE; BACTERIUM CULTURE; COLONY FORMING UNIT; CONTROLLED STUDY; EPITHELIUM CELL; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MIDDLE EAR; MOLECULAR MECHANICS; NONHUMAN; NUCLEOTIDE SEQUENCE; OTITIS MEDIA; PHENOTYPE; PRESSURE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; SIMULATION; STREPTOCOCCUS PNEUMONIAE; TUBE;

ADHESINS, BACTERIAL; BACTERIAL PROTEINS; CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; EAR, MIDDLE; EPITHELIAL CELLS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; HUMANS; LIPOPROTEINS; MODELS, BIOLOGICAL; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; OTITIS MEDIA; PHENOTYPE; PYRUVATE OXIDASE; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS PNEUMONIAE; STREPTOLYSINS; VIRULENCE FACTORS;

EID: 69249217771     PISSN: 01655876     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijporl.2009.07.007     Document Type: Article

References (39)

1. Jedrzejas M.J. Pneumococcal virulence factors: structure and function. Microbiol. Mol. Biol. Rev. 65 (2001) 187-207
2. Di Guilmi A.M., and Dessen A. New approaches towards the identification of antibiotic and vaccine targets in Streptococcus pneumoniae. EMBO Rep. 3 (2002) 728-734
3. Bogaert D., Hermans P.W., Adrian P.V., Rumke H.C., and de Groot R. Pneumococcal vaccines: an update on current strategies. Vaccine 22 (2004) 2209-2220
4. Kadioglu A., Weiser J.N., Paton J.C., and Andrew P.W. The role of Streptococcus pneumoniae virulence factors in host respiratory colonization and disease. Nat. Rev. Microbiol. 6 (2008) 288-301
5. Hava D.L., LeMieux J., and Camilli A. From nose to lung: the regulation behind Streptococcus pneumoniae virulence factors. Mol. Microbiol. 50 (2003) 1103-1110
6. Orihuela C.J., Gao G., Francis K.P., Yu J., and Tuomanen E.I. Tissue-specific contributions of pneumococcal virulence factors to pathogenesis. J. Infect. Dis. 190 (2004) 1661-1669
7. Claverys J.P., Prudhomme M., Mortier-Barriere I., and Martin B. Adaptation to the environment: Streptococcus pneumoniae, a paradigm for recombination-mediated genetic plasticity?. Mol. Microbiol. 35 (2000) 251-259
8. Dowson C.G., Barcus V., King S., Pickerill P., Whatmore A., and Yeo M. Horizontal gene transfer and the evolution of resistance and virulence determinants in Streptococcus. Soc. Appl. Bacteriol. Symp. Ser. 26 (1997) 42S-51S
9. Hakenbeck R., Balmelle N., Weber B., Gardes C., Keck W., and de Saizieu A. Mosaic genes and mosaic chromosomes: intra- and interspecies genomic variation of Streptococcus pneumoniae. Infect. Immun. 69 (2001) 2477-2486
10. Weiser J.N., Austrian R., Sreenivasan P.K., and Masure H.R. Phase variation in pneumococcal opacity: relationship between colonial morphology and nasopharyngeal colonization. Infect. Immun. 62 (1994) 2582-2589
11. Saluja S.K., and Weiser J.N. The genetic basis of colony opacity in Streptococcus pneumoniae: evidence for the effect of box elements on the frequency of phenotypic variation. Mol. Microbiol. 16 (1995) 215-227
12. Weiser J.N. Phase variation in colony opacity by Streptococcus pneumoniae. Microb. Drug Resist. 4 (1998) 129-135
13. Cundell D.R., Pearce B.J., Sandros J., Naughton A.M., and Masure H.R. Peptide permeases from Streptococcus pneumoniae affect adherence to eucaryotic cells. Infect. Immun. 63 (1995) 2493-2498
14. Kim J.O., and Weiser J.N. Association of intrastrain phase variation in quantity of capsular polysaccharide and teichoic acid with the virulence of Streptococcus pneumoniae. J. Infect. Dis. 177 (1998) 368-377
15. Gould J.M., and Weiser J.N. The inhibitory effect of C-reactive protein on bacterial phosphorylcholine platelet-activating factor receptor-mediated adherence is blocked by surfactant. J. Infect. Dis. 186 (2002) 361-371
16. Kim J.O., Romero-Steiner S., Sorensen U.B., Blom J., Carvalho M., Barnard S., Carlone G., and Weiser J.N. Relationship between cell surface carbohydrates and intrastrain variation on opsonophagocytosis of Streptococcus pneumoniae. Infect. Immun. 67 (1999) 2327-2333
17. Serrano I., Melo-Cristino J., and Ramirez M. Heterogeneity of pneumococcal phase variants in invasive human infections. BMC Microbiol. 6 (2006) 1-6
18. Li-Korotky H.S., Banks J.M., Lo C.Y., Zeng F.R., Stolz D.B., Swarts J.D., and Doyle W.J. Interaction of pneumococcal phase variation and middle ear pressure/gas composition: an in vitro model of simulated otitis media. Microb. Pathog. 45 (2008) 201-206
19. Chun Y.M., Moon S.K., Lee H.Y., Webster P., Brackmann D.E., Rhim J.S., and Lim D.J. Immortalization of normal adult human middle ear epithelial cells using a retrovirus containing the E6/E7 genes of human papillomavirus type 16. Ann. Otol. Rhinol. Laryngol. 111 (2002) 507-517
20. Schmittgen T.D., Zakrajsek B.A., Mills A.G., Gorn V., Singer M.J., and Reed M.W. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Anal. Biochem. 285 (2000) 194-204
21. Sampson J.S., Furlow Z., Whitney A.M., Williams D., Facklam R., and Carlone G.M. Limited diversity of Streptococcus pneumoniae psaA among pneumococcal vaccine serotypes. Infect. Immun. 65 (1997) 1967-1971
22. Jado I., Fenoll A., Casal J., and Perez A. Identification of the psaA gene, coding for pneumococcal surface adhesin A, in viridans group streptococci other than Streptococcus pneumoniae. Clin. Diagn. Lab. Immunol. 8 (2001) 895-898
23. Morrison K.E., Lake D., Crook J., Carlone G.M., Ades E., Facklam R., and Sampson J.S. Confirmation of psaA in all 90 serotypes of Streptococcus pneumoniae by PCR and potential of this assay for identification and diagnosis. J. Clin. Microbiol. 38 (2000) 434-437
24. LeMessurier K.S., Ogunniyi A.D., and Paton J.C. Differential expression of key pneumococcal virulence genes in vivo. Microbiology 152 (2006) 305-311
25. Spellerberg B., Cundell D.R., Sandros J., Pearce B.J., Idanpaan-Heikkila I., Rosenow C., and Masure H.R. Pyruvate oxidase, as a determinant of virulence in Streptococcus pneumoniae. Mol. Microbiol. 19 (1996) 803-813
26. Pericone C.D., Park S., Imlay J.A., and Weiser J.N. Factors contributing to hydrogen peroxide resistance in Streptococcus pneumoniae include pyruvate oxidase (SpxB) and avoidance of the toxic effects of the Fenton reaction. J. Bacteriol. 185 (2003) 6815-6825
27. Regev-Yochay G., Trzcinski K., Thompson C.M., Lipsitch M., and Malley R. SpxB is a suicide gene of Streptococcus pneumoniae and confers a selective advantage in an in vivo competitive colonization model. J. Bacteriol. 189 (2007) 6532-6539
28. Belanger A.E., Clague M.J., Glass J.I., and Leblanc D.J. Pyruvate oxidase is a determinant of Avery's rough morphology. J. Bacteriol. 186 (2004) 8164-8171
29. Takoudes T.G., and Haddad Jr. J. Hydrogen peroxide in acute otitis media in guinea pigs. Laryngoscope 107 (1997) 206-210
30. Overweg K., Pericone C.D., Verhoef G.G., Weiser J.N., Meiring H.D., De Jong A.P., De Groot R., and Hermans P.W. Differential protein expression in phenotypic variants of Streptococcus pneumoniae. Infect. Immun. 68 (2000) 4604-4610
31. Sato K., Quartey M.K., Liebeler C.L., Le C.T., and Giebink G.S. Roles of autolysin and pneumolysin in middle ear inflammation caused by a type 3 Streptococcus pneumoniae strain in the chinchilla otitis media model. Infect. Immun. 64 (1996) 1140-1145
32. Cockeran R., Anderson R., and Feldman C. Pneumolysin in the immunopathogenesis and treatment of pneumococcal disease. Expert Rev. Anti Infect. Ther. 1 (2003) 231-239
33. Paton J.C., Berry A.M., Lock R.A., Hansman D., and Manning P.A. Cloning and expression in Escherichia coli of the Streptococcus pneumoniae gene encoding pneumolysin. Infect. Immun. 54 (1986) 50-55
34. Paton J.C., Andrew P.W., Boulnois G.J., and Mitchell T.J. Molecular analysis of the pathogenicity of Streptococcus pneumoniae: the role of pneumococcal proteins. Annu. Rev. Microbiol. 47 (1993) 89-115
35. El-Rachkidy R.G., Davies N.W., and Andrew P.W. Pneumolysin generates multiple conductance pores in the membrane of nucleated cells. Biochem. Biophys. Res. Commun. 368 (2008) 786-792
36. Ronda C., Garcia J.L., Garcia E., Sanchez-Puelles J.M., and Lopez R. Biological role of the pneumococcal amidase. Cloning of the lytA gene in Streptococcus pneumoniae. Eur. J. Biochem. 164 (1987) 621-624
37. Lopez R., Garcia E., Garcia P., and Garcia J.L. The pneumococcal cell wall degrading enzymes: a modular design to create new lysins?. Microb. Drug Resist. 3 (1997) 199-211
38. Weiser J.N., Markiewicz Z., Tuomanen E.I., and Wani J.H. Relationship between phase variation in colony morphology, intrastrain variation in cell wall physiology, and nasopharyngeal colonization by Streptococcus pneumoniae. Infect. Immun. 64 (1996) 2240-2245
39. Hirst R.A., Gosai B., Rutman A., Guerin C.J., Nicotera P., Andrew P.W., and O'Callaghan C. Streptococcus pneumoniae deficient in pneumolysin or autolysin has reduced virulence in meningitis. J. Infect. Dis. 197 (2008) 744-751