Esterified cholesterol is highly localized to Bruch's membrane, as revealed by lipid histochemistry in wholemounts of human choroid

Journal of Histochemistry and Cytochemistry

Volumn 57, Issue 8, 2009, Pages 731-739

  Rudolf, Martin ^a,b   Curcio, Christine A ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b UNIVERSITY HOSPITAL SCHLESWIG HOLSTEIN   (Germany)

Author keywords

Age related maculopathy; Aging; Bruch's membrane; Cholesterol; Lipids; Wholemount

Indexed keywords

BODIPY 493 503; CHOLESTEROL ESTER; FILIPIN; FLUORESCENT DYE; LIPID; NILE RED; OIL RED O; UNCLASSIFIED DRUG;

AGED; ARTICLE; BRUCH MEMBRANE; CHOROID; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; HISTOCHEMISTRY; HUMAN; HUMAN TISSUE; LIPID ANALYSIS; LIPID STORAGE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RELIABILITY; RETINA MACULA AGE RELATED DEGENERATION; STAINING; CYTOCHEMISTRY; METABOLISM; RETINA;

AGED; AGED, 80 AND OVER; BRUCH MEMBRANE; CHOLESTEROL ESTERS; CHOROID; FLUORESCENT DYES; HISTOCYTOCHEMISTRY; HUMANS; RETINA; STAINING AND LABELING;

EID: 69249213701     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2009.953448     Document Type: Article

References (34)

1. Adams CWM, Bayliss OB (1975) Lipid histochemistry. In Glick D, Rosenbaum RM, eds. Techniques of Biochemical and Biophysical Morphology. 2nd ed. New York, John Wiley and Sons, 100-156
2. Beatty S, Koh H, Phil M, Henson D, Boulton M (2000) The role of oxidative stress in the pathogenesis of age-related macular degeneration. Surv Ophthalmol 45:115-134
3. Bittman R, Fischkoff SA (1972) Fluorescence studies of the binding of the polyene antibiotics filipin 3, amphotericin B, nystatin, and lagosin to cholesterol. Proc Natl Acad Sci USA 69:3795-3799
4. Chong NH, Keonin J, Luthert PJ, Frennesson CI, Weingeist DM, Wolf RL, Mullins RF, et al. (2005) Decreased thickness and integrity of the macular elastic layer of Bruch's membrane correspond to the distribution of lesions associated with age-related macular degeneration. Am J Pathol 166:241-251
5. Curcio CA, Millican CL, Bailey T, Kruth HS (2001) Accumulation of cholesterol with age in human Bruch's membrane. Invest Ophthalmol Vis Sci 42:265-274
6. Curcio CA, Presley JB, Malek G, Medeiros NE, Avery DV, Kruth HS (2005) Esterified and unesterified cholesterol in drusen and basal deposits of eyes with age-related maculopathy. Exp Eye Res 81:731-741
7. Curcio CA, Sloan KR, Kalina RE, Hendrickson AE (1990) Human photoreceptor topography. J Comp Neurol 292:497-523
8. Edwards AO, Malek G (2007) Molecular genetics of AMD and current animal models. Angiogenesis 10:119-132
9. Ethier CR, Johnson M, Ruberti J (2004) Ocular biomechanics and biotransport. Annu Rev Biomed Eng 6:249-273
10. Gocze PM, Freeman DA (1994) Factors underlying the variability of lipid droplet fluorescence in MA-10 Leydig tumor cells. Cytometry 17:151-158
11. Greenspan P, Fowler SD (1985) Spectrofluorometric studies of the lipid probe, nile red. J Lipid Res 26:781-789
12. Greenspan P, Mayer EP, Fowler SD (1985) Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol 100:965-973
13. Hageman GS, Anderson DH, Johnson LV, Hancox LS, Taiber AJ, Hardisty LI, Hageman JL, et al. (2005) A common haplotype in the complement regulatory gene factor H (HF1/CFH) predisposes individuals to age-related macular degeneration. Proc Natl Acad Sci USA 102:7227-7232
14. Haimovici R, Gantz DL, Rumelt S, Freddo TF, Small DM (2001) The lipid composition of drusen, Bruch's membrane, and sclera by hot stage polarizing light microscopy. Invest Ophthalmol Vis Sci 42:1592-1599
15. Hayat MA (2002) Microscopy, Immunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy. 1st ed. New York, Springer, 68
16. Hogan MJ (1972) Role of the retinal pigment epithelium in macular disease. Trans Am Acad Ophthalmol Otolaryngol 76:64-80
17. Holz FG, Schuett F, Pauleikhoff D, Bird AC (2004) AMD pathophysiology. In Holz FG, Pauleikhoff D, Spaide RF, Bird AC, eds. Age-related Macula Degeneration. Berlin, Heidelberg, Springer-Verlag, 32-46
18. Holz FG, Sheraidah G, Pauleikhoff D, Bird AC (1994) Analysis of lipid deposits extracted from human macular and peripheral Bruch's membrane. Arch Ophthalmol 112:402-406
19. Huang JD, Curcio CA, Johnson M (2008) Morphometric analysis of lipoprotein-like particle accumulation in aging human macular Bruch's membrane. Invest Ophthalmol Vis Sci 49:2721-2727
20. Huang JD, Presley JB, Chimento MF, Curcio CA, Johnson M (2007) Age-related changes in human macular Bruch's membrane as seen by quick-freeze/deep-etch. Exp Eye Res 85:202-218
21. Koopman R, Schaart G, Hesselink MK (2001) Optimisation of oil red O staining permits combination with immunofluorescence and automated quantification of lipids. Histochem Cell Biol 116:63-68
22. Kruth HS (1997) The fate of lipoprotein cholesterol entering the arterial wall. Curr Opin Lipidol 8:246-252
23. Kruth HS, Cupp JE, Khan MA (1987) Method for detection and isolation of cholesteryl ester-containing "foam" cells using flow cytometry. Cytometry 8:146-152
24. Lengyel I, Tufail A, Hosaini HA, Luthert P, Bird AC, Jeffery G (2004) Association of drusen deposition with choroidal intercapillary pillars in the aging human eye. Invest Ophthalmol Vis Sci 45:2886-2892
25. Li CM, Chung BH, Presley JB, Malek G, Zhang X, Dashti N, Li L, et al. (2005a) Lipoprotein-like particles and cholesteryl esters in human Bruch's membrane: initial characterization. Invest Ophthalmol Vis Sci 46:2576-2586
26. Li CM, Presley JB, Zhang X, Dashti N, Chung BH, Medeiros NE, Guidry C, et al. (2005b) Retina expresses microsomal triglyceride transfer protein: implications for age-related maculopathy. J Lipid Res 46:628-640
27. Marmorstein AD, Marmorstein LY, Sakaguchi H, Hollyfield JG (2002) Spectral profiling of autofluorescence associated with lipofuscin, Bruch's Membrane, and sub-RPE deposits in normal and AMD eyes. Invest Ophthalmol Vis Sci 43:2435-2441
28. McMenamin PG (2000) Optimal methods for preparation and immunostaining of iris, ciliary body, and choroidal wholemounts. Invest Ophthalmol Vis Sci 41:3043-3048
29. Norman AW, Demel RA, de Kruyff B, van Deenen LL (1972) Studies on the biological properties of polyene antibiotics. Evidence for the direct interaction of filipin with cholesterol. J Biol Chem 247:1918-1929
30. Pauleikhoff D, Harper CA, Marshall J, Bird AC (1990) Aging changes in Bruch's membrane. A histochemical and morphologic study. Ophthalmology 97:171-178
31. Rudolf M, Li CM, Chimento MF, Curcio CA (2007) Topography of esterified cholesterol deposition in human Bruch membrane wholemounts. Invest Ophthalmol Vis Sci 48:2184
32. Sheu HM, Tsai JC, Lin TK, Wong TW, Lee JY (2003) Modified Nile red staining method for improved visualization of neutral lipid depositions in stratum corneum. J Formos Med Assoc 102:656-660
33. Wang L, Li CM, Rudolf M, Belyaeva OV, Chung BH, Messinger JD, Kedishvili NY, et al. (2009) Lipoprotein particles of intraocular origin in human Bruch membrane: an unusual lipid profile. Invest Ophthalmol Vis Sci 50:870-877
34. Zarbin MA (1998) Age-related macular degeneration: review of pathogenesis. Eur J Ophthalmol 8:199-206