Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A;Comparação entre as técnicas de RT-PCR convencional e RT-PCR em tempo real para a detecção do metapneumovírus aviários subtipo A

Ciencia Rural

Volumn 39, Issue 5, 2009, Pages 1445-1451

  Ferreira, Helena Lage ^a   Spilki, Fernando Rosado ^b   dos Santos, Márcia Mercês Aparecida Bianchi ^c   de Almeida, Renata Servan ^d   Arns, Clarice Weis ^c  

^a Belgian National Institute for Veterinary Research   (Belgium)

^b UNIVERSIDADE FEEVALE   (Brazil)

^c UNIVERSITY OF CAMPINAS   (Brazil)

^d CIRAD   (France)

Author keywords

Avian metapneumovirus; F; G; N genes; Real time RT PCR; RT PCR

Indexed keywords

AVIAN METAPNEUMOVIRUS; METAPNEUMOVIRUS; PARAMYXOVIRIDAE; SUBGROUP A;

EID: 69249148697     PISSN: 01038478     EISSN: 16784596     Source Type: Journal    
DOI: 10.1590/S0103-84782009005000057     Document Type: Article

References (20)

1. ARNS, C.W.; HAFEZ, M.H. Swollen head syndrome in poultry flocks in Brazil. In: WESTERN POULTRY DISEASE CONFERENCE, 41., 1992, Sacramento, USA. Proceedings... Davis, CA: Conference & Event Services, University of California, 1992. p.81-84.
2. BARIK, S. Transcription of human respiratory syncytial virus genome RNA in vitro: requirement of cellular factor(s). Journal of Virology, v.66, p.6813-6818, 1992.
3. BÄYON-AUBOYER, M.H. et al. Comparison of F-, G- and N-based RT-PCR protocols with conventional virological procedures for the detection and typing of turkey rhinotracheitis virus. Archives of Virology, v.144, n.6, p.1091-1109, 1999. Disponível em: . Doi: 10.1007/s007050050572.
4. CECCHINATO, M. et al. Design, validation, and absolute sensitivity of a novel test for the molecular detection of avian pneumovirus. Journal of Veterinary Diagnostic Investigation, v.16, n.6, p.582-585, 2004.
5. CHOI, J.H. et al. Development of real-time PCR assays for detection and quantification of human bocavirus. Journal of Clinical Microbiology, v.42, n.3, p.249-253, 2008. Disponível em: . Doi: 10.1016/j.jcv.2008.02.010.
6. COOK, J.K.; CAVANAGH, D. Detection and differentiation of avian pneumoviruses (metapneumoviruses). Avian Pathology, v.31, n.2, p.117-132, 2002.
7. D'ARCE, R.C. et al. Subtyping of new Brazilian avian metapneumovirus isolates from chickens and turkeys by reverse transcriptase-nested-polymerase chain reaction. Avian Pathology, v.34, n.2, p.133-136, 2005.
8. DANI, M.A. et al. Molecular characterization of Brazilian avian pneumovirus isolates: comparison between immunochemiluminescent Southern blot and nested PCR. Journal of Virological Methods, v.79, n.2, p.237-241, 1999. Disponível em: . Doi: 10.1016/S0166-0934(99)00020-8.
9. FAUQUET, C.M. et al. Virus taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses. Amsterdam: Elsevier Academic, 2005. 1162p.
10. FERREIRA, H.L. et al. Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells. Antiviral Research, v.74, n.1, p.77-81, 2007. Disponível em: . Doi: 10.1016/j.antiviral.2006.12.002.
11. GIRAUD, P. et al. Turkey rhinotracheitis in France: preliminary investigations on a ciliostatic virus. Veterinary Record, v.119, n.24, p.606-607, 1986.
12. GOUGH, R.E. Avian pneumoviruses. In: SAIF, M. et al. Diseases of poultry. Ames: Iowa State, 2003. p.92-99.
13. GUIONIE, O. et al. Laboratory evaluation of a quantitative real-time reverse transcription PCR assay for the detection and identification of the four subgroups of avian metapneumovirus. Journal of Virological Methods, v.139, n.2, p.150-158, 2007. Disponível em: . Doi: 10.1016/j.jviromet.2006.09.022.
14. JUHASZ, K.; EASTON, A.J. Extensive sequence variation in the attachment (G) protein gene of avian pneumovirus: evidence for two distinct subgroups. Journal of General Virology, v.75 (Pt 11), p.2873-2880, 1994. Disponível em: . Doi: 10.1099/0022-1317-75-11-2873.
15. KEIGHTLEY, M.C. et al. Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR. Journal of Medical Virology, v.77, n.4, p.602-608, 2005. Disponível em: . Doi: 10.1002/jmv.20498.
16. LOISY, F. et al. Real-time RT-PCR for norovirus screening in shellfish. Journal of Virological Methods, v.123, n.1, p.1-7, 2005.
17. MAERTZDORF, J. et al. Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages. Journal of Clinical Microbiology, v.42, p.981-986, 2004. Disponível em: . Doi: 10.1128/JCM.42.3.981-986.2004.
18. OTSUKI, K. et al. Demonstration of serum-neutralising antibody to turkey rhinotracheitis virus in serum from chicken flocks in Japan. Journal of Veterinary Medical Science, v.58, n.9, p.869-874, 1996.
19. PABBARAJU, K. et al. Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR. Journal of Clinical Virology, v.40, n.3, p.186-192, 2007. m .Doi:10.1016/j.jcv.2007.08.004.
20. REED, J.I.; MUENCH, H. A simple method for estimating fifth percent endpoints. American Journal Hygiene, v.27, p.493-497, 1938.