ITS-RFLP analysis, an efficient tool for differentiation of Bursaphelenchus species

Nematology

Volumn 11, Issue 5, 2009, Pages 649-668

  Burgermeister, Wolfgang ^a   Braasch, Helen ^b   Metge, Kai ^a   Gu, Jianfeng ^c   Schröder, Thomas ^a   Woldt, Elvira ^a  

^a INSTITUTE FOR EPIDEMIOLOGY AND PATHOGEN DIAGNOSTICS   (Germany)

^b Ningbo Entry Exit Inspection and Quarantine Bureau ^*  (Germany)

^c Ningbo Entry Exit Inspection and Quarantine Bureau   (China)

Author keywords

Intraspecific types; ITS microheterogeneity; Molecular diagnosis; Pine wood nematode (PWN); Species identification

Indexed keywords

DNA; ENZYME ACTIVITY; GENETIC ANALYSIS; INFORMATION MANAGEMENT; INTRASPECIFIC INTERACTION; NEMATODE; NEW SPECIES; PATHOGENICITY; RELATEDNESS; SPECIES DIVERSITY;

APHELENCHOIDES; BURSAPHELENCHUS; BURSAPHELENCHUS LEONI; BURSAPHELENCHUS MUCRONATUS; BURSAPHELENCHUS SEXDENTATI; BURSAPHELENCHUS XYLOPHILUS; NEMATODA;

EID: 69249117799     PISSN: 13885545     EISSN: 15685411     Source Type: Journal    
DOI: 10.1163/156854108/399182     Document Type: Article

References (64)

1. Akbulut, S., Braasch, H., Baysal, I., Brandstetter, M. & Burgermeister, W. (2007). Description of Bursa-phelenchus anamurius sp. n. (Nematoda: Parasitaphelenchi-dae) from Pinus brutia in Turkey. Nematology 9, 859-867.
2. Beckenbach, K., Blaxter, M. & Webster, J.M. (1999). Phylogeny of Bursaphelenchus species derived from analysis of ribosomal internal transcribed spacer DNA sequences. Nematology 1, 539-548.
3. Blok, V.C., Malloch, G., Harrower, B., Phillips, M.S. & Vrain, T.C. (1998). Intraspecific variation in ribosomal DNA in populations of the potato cyst nematode Globodera pallida. Journal of Nematology 30, 262-274.
4. Blouin, M.S. (2002). Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. International Journal of Parasitology 32, 527-531.
5. Boutsika, K., Brown, D.J.F., Phillips, M.S. & Blok, V.C. (2004). Molecular characterisation of the ribosomal DNA of Paratrichodorus macrostylus, P. pachydermus, Tri-chodorus primitivus and T. similis (Nematoda: Trichodori-dae). Nematology 6, 641-654.
6. Braasch, H. (1991). Erster Nachweis von Bursaphelenchus mucronatus Mamiya und Enda, 1979 in der DDR und sein Vorkommen in Holzimporten aus der UdSSR nebst Ergänzungen zur Beschreibung dieser Art. Archiv für Phy-topathologie und Pflanzenschutz 27, 209-218.
7. Braasch, H. (2008). The enlargement of the xylophilus group in the genus Bursaphelenchus. In: Mota, M.M. & Vieira, P. (Eds). Pine wilt disease: a worldwide threat to forest ecosystems, Berlin, Germany, Springer, pp. 139-149.
8. Braasch, H. & Braasch-Bidasak, R. (2002). First record of the genus Bursaphelenchus Fuchs, 1937 in Thailand and description of B. thailandae sp. n. (Nematoda: Parasitaphe-lenchidae). Nematology 4, 853-863. (Pubitemid 36021873)
9. Braasch, H. & Burgermeister, W. (2002). Bursaphelen-chus rainulfi sp. n. (Nematoda: Parasitaphelenchidae), first record of the genus Bursaphelenchus Fuchs, 1937 from Malaysia. Nematology 4, 971-978. (Pubitemid 36242537)
10. Braasch, H., Burgermeister, W. & Hoyer, U. (1998). Diversity within the species Bursaphelenchus mucronatus worldwide. Proceedings of the International Nematology Symposium, Dundee, UK, 4-9 August 1998. Nematologica 44, 465-466.
11. Braasch, H., Metge, K. & Burgermeister, W. (1999). [Bursaphelenchus species (Nematoda, Parasitaphelenchidae) found in coniferous trees in Germany and their ITS-RFLP patterns.] Nachrichtenblatt des Deutschen Pflanzenschutz-dienstes 51, 312-320.
12. Braasch, H., Tomiczek, Ch., Metge, K., Hoyer, U., Burgermeister, W., Wulfert, I. & Schönfeld, U. (2001). Records of Bursaphelenchus spp. (Nematoda: Parasi-taphelenchidae) in coniferous timber imported from the Asian part of Russia. Forest Pathology 31, 129-140.
13. Braasch, H., Gu, J., Burgermeister, W. & Zhang, J. (2004a). Bursaphelenchus doui sp. n. (Nematoda: Parasita-phelenchidae) in packaging wood from Taiwan and South Korea - a new species of the xylophilus group. Russian Journal of Nematology 12, 19-27.
14. Braasch, H., Schönfeld, U., Polomski, J. & Burger-meister, W. (2004b). Bursaphelenchus vallesianus sp. n. - a new species of the Bursaphelenchus sexdentati group (Nematoda: Parasitaphelenchidae). Nematologia Mediterranea 32, 71-79.
15. Braasch, H., Brandstetter, M. & Burgermeister, W. (2006a). Supplementary characters of Bursaphelenchus lini Braasch, 2004 (Nematoda: Parasitaphelenchidae) and remarks on this nematode. Zootaxa 1141, 55-61.
16. Braasch, H., Burgermeister, W., Schönfeld, U., Metge, K. & Brandstetter, M. (2006b). Bursaphelen-chus hildegardae sp. n. (Nematoda: Parasitaphelenchidae) - a new species belonging to the 'eggersi' group. Journal of Ne-matode Morphology and Systematics 9, 27-38.
17. Braasch, H., Burgermeister, W., Schröder, T., Apel, K.-H. & Brandstetter, M. (2006c). [Occurrence of Bursaphelenchus eremus Rühm, 1956 (Nematoda: Parasita-phelenchidae) on oaks in State Brandenburg/Germany and supplementary species description.] Nachrichtenblatt des Deutschen Pflanzenschutzdienstes 58, 148-153.
18. Braasch, H., Gu, J., Burgermeister, W., Brandstet-ter, M. & Metge, K. (2006d). Description of Ruehmaphe-lenchus asiaticus sp. n. (Nematoda: Aphelenchoididae) and remarks on the genus Ruehmaphelenchus. Journal of Nema-tode Morphology and Systematics 9, 39-48.
19. Braasch, H., Gu, J., Burgermeister, W., Brandstet-ter, M. & Metge, K. (2007a). Bursaphelenchus africanus sp. n. (Nematoda: Parasitaphelenchidae) - found in packaging wood exported from South Africa to Ningbo/China. Journal of Nematode Morphology and Systematics 9, 71-81.
20. Braasch, H., Gu, J. & Brandstetter, M. (2007b). Bursa-phelenchus burgermeisteri sp. n. (Nematoda: Parasitaphelen-chidae) in packaging wood from Japan - a second species of the 'africanus' group. Journal of Nematode Morphology and Systematics 10, 39-48.
21. Burgermeister, W., Metge, K., Braasch, H. & Buch-bach, E. (2005a). ITS-RFLP patterns for differentiation of 26 Bursaphelenchus species (Nematoda: Parasitaphelenchi-dae) and observations on their distribution. Russian Journal of Nematology 13, 29-42.
22. Burgermeister, W., Gu, J. & Braasch, H. (2005b). Bur-saphelenchus arthuri sp. n. (Nematoda: Parasitaphelenchi-dae) in packaging wood from Taiwan and South Korea - a new species belonging to the 'fungivorus' group. Journal of Nematode Morphology and Systematics 8, 7-17.
23. De Luca, F., Reyes, A., Grunder, J., Kunz, P., Agostinelli, A., De Giorgi, C. & Lamberti, F. (2004). Characterization and sequence variation in the rDNA region of six nematode species of the genus Longidorus (Nematoda). Journal of Nematology 36, 147-152.
24. Fang, Y., Zhuo, K. & Zhao, J. (2002). Description of Bur-saphelenchus aberrans n. sp. (Nematoda: Parasitaphelenchi-dae) isolated from pine wood in Guangdong Province, China. Nematology 4, 791-794.
25. Ferris, V.R., Ferris, J.M. & Faghihi, J. (1993). Variation in spacer ribosomal DNA in some cyst-forming species of plant parasitic nematodes. Fundamental and Applied Nematology 16, 177-184.
26. Gu, J., Zhang, J., Braasch, H. & Burgermeister, W. (2005). Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore - a new species of the B. xylophilus group. Zootaxa 988, 1-12. (Pubitemid 43910573)
27. Gu, J., Braasch, H., Burgermeister, W. & Zhang, J. (2006a). Records of Bursaphelenchus spp. intercepted in imported packaging wood at Ningbo, China. Forest Pathology 36, 323-333.
28. Gu, J., Braasch, H., Burgermeister, W., Brandstet-ter, M. & Zhang, J. (2006b). Description of Bursaphelen-chus yongensis sp. n. (Nematoda: Parasitaphelenchidae) isolated from Pinus massoniana in China. Russian Journal of Nematology 14, 91-99.
29. Gu, J., Braasch, H., Zheng, W., Burgermeister, W. & Lin, M. (2007). Bursaphelenchus obeche sp. n. (Nematoda: Parasitaphelenchidae) found in packaging wood exported from Indonesia to Ningbo, China. Journal of Nematode Morphology and Systematics 10, 165-175.
30. Gu, J., Zheng, W., Braasch, H. & Burgermeister, W. (2008). Description of Bursaphelenchus macromucronatus sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Taiwan and India - a new species of the 'xylophilus' group. Journal of Nematode Morphology and Systematics 11, 31-40.
31. Hillis, D.M. & Dixon, M.T. (1991). Ribosomal DNA: molecular evolution and phylogenetic inference. Quarterly Review of Biology 66, 411-453.
32. Hoyer, U., Burgermeister, W. & Braasch, H. (1998). Identification of Bursaphelenchus species (Nematoda, Aphe-lenchoididae) on the basis of amplified ribosomal DNA (ITS-RFLP). Nachrichtenblatt des Deutschen Pflanzenschutzdien-stes 50, 273-277.
33. Hübschen, J., Kling, L., Ipach, U., Zinkernagel, V., Bosselut, N., Esmenjaud, D., Brown, D.J.F. & Neil-son, R. (2004). Validation of the specificity and sensitivity of species-specific primers that provide a reliable molecular diagnostic for Xiphinema diversicaudatum, X. index and X. vuit-tenezi. European Journal of Plant Pathology 110, 779-788.
34. Hunt, D.J. (2008). A checklist of the Aphelenchoidea (Nema-toda: Tylenchina). Journal of Nematode Morphology and Sys-tematics 10 (2007), 99-135.
35. Iwahori, H., Tsuda, K., Kanzaki, N., Izui, K. & Futai, K. (1998). PCR-RFLP and sequencing analysis of ribosomal DNA of Bursaphelenchus nematodes related to pine wilt disease. Fundamental and Applied Nematology 21, 655-666.
36. Iwahori, H., Kanzaki, N. & Futai, K. (2000). A simple, polymerase chain reaction-restriction fragment length polymorphism-aided diagnosis method for pine wilt disease. Forest Pathology 30, 157-164.
37. Kang, J.S., Choi, K.S., Shin, S.C., Moon, I.S., Lee, S.G. & Lee, S.H. (2004). Development of an efficient PCR-based diagnosis protocol for the identification of the pine wood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). Nematology 6, 279-285.
38. Kanzaki, N. & Futai, K. (2003). Description and phylogeny of Bursaphelenchus luxuriosae n. sp. (Nematoda: Aphelen-choididae) isolated from Acalolepta luxuriosa (Coleoptera: Cerambycidae). Nematology 5, 565-572.
39. Kanzaki, N. & Futai, K. (2005). Description of Bursaphe-lenchus parvispicularis n. sp. (Nematoda: Parasitaphelenchi-dae) isolated from a dead oak tree, Quercus mongolica var. grosseserrata. Nematology 7, 751-759.
40. Kanzaki, N. & Futai, K. (2007). Isolation of Bursaphelen-chus sinensis (Nematoda: Parasitaphelenchidae) from dead Japanese black pine, Pinus thunbergii Parl. in Japan. Journal of Nematode Morphology and Systematics 9 (2006), 129-136.
41. Kanzaki, N., Maehara, N. & Masuya, H. (2007). Bursa-phelenchus clavicauda n. sp. (Nematoda: Parasitaphelenchi-dae) isolated from Cryphalus sp. emerging from a dead Cas-tanopsis cuspida (Thunb.) Schottky var. sieboldii (Makino) Nakai in Ishigaki Island, Okinawa, Japan. Nematology 9, 759-769.
42. Kanzaki, N., Aikawa, T., Maehara, N. & Matsumoto, K. (2008). Bursaphelenchus doui Braasch, Gu, Burgermeister & Zhang, 2005 (Aphelenchida: Parasitaphelenchidae), an associate of Monochamus subfasciatus Bates (Coleoptera: Ce-rambycidae) and Pinus densiflora Sieb. & Zucc. Nematology 10, 69-78.
43. Lange, C., Burgermeister, W., Metge, K. & Braasch, H. (2007). Phylogenetic analysis of isolates of the Bursa-phelenchus sexdentati group using ribosomal intergenic transcribed spacer DNA sequences. Journal of Nematode Morphology and Systematics 9 (2006), 95-109.
44. Li, H., Trinh, P.Q., Shen, P., Waeyenberge, L. & Moens, M. (2008). Bursaphelenchus chengi sp. n. (Nema-toda: Parasitaphelenchidae) isolated at Nanjing, China, from packaging wood from Taiwan. Nematology 10, 335-346.
45. Magnusson, C. & Kulinich, O.A. (1996). A taxonomic appraisal of the original description, morphology and status of Bursaphelenchus kolymensis Korentchenko, 1980 (Aphe-lenchida: Aphelenchoididae). Russian Journal of Nematology 4, 155-161.
46. Marinari Palmisaro, A., Ambrogioni, L., Tomiczek, C. & Brandstetter, M. (2004). Bursaphelenchus sinensis sp. n. and B. thailandae Braasch et Braasch-Bidasak in packaging wood from China. Nematologia Mediterranea 32, 57-65.
47. Matsunaga, K. & Togashi, K. (2004). A simple method for discriminating Bursaphelenchus xylophilus and B. mucro-natus by species-specific polymerase chain reaction primer pairs. Nematology 6, 273-277.
48. Mota, M.M., Braasch, H., Bravo, M.A., Penas, A.C., Burgermeister, W., Metge, K. & Sousa, E. (1999). First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1, 727-734.
49. Nadler, S.A., Hoberg, E.P., Hudspeth, D.S.S. & Rickard, L.G. (2000). Relationships of Nematodirus species and Nematodirus battus isolates (Nematoda: Tri-chostrongyloidea) based on nuclear ribosomal DNA sequences. Journal of Parasitology 86, 588-601.
50. Penas, A.C., Correia, P., Bravo, M.A., Mota, M. & Tenreiro, R. (2004). Species of Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) associated with maritime pine in Portugal. Nematology 6, 437-453.
51. Penas, A.C., Metge, K., Mota, M. & Valadas, V. (2006). Bursaphelenchus antoniae sp. n. (Nematoda: Parasitaphelen-chidae) associated with Hylobius sp. from Pinus pinaster in Portugal. Nematology 8, 659-669.
52. Polomski, J., Schönfeld, U., Braasch, H., Dobbertin, M., Burgermeister, W. & Rigling, D. (2005). Occurrence of Bursaphelenchus species in declining Pinus sylvestris in a dry Alpine valley in Switzerland. Forest Pathology 35, 1-9.
53. Powers, T.O., Todd, T.C., Burnell, A.M., Murray, P.C.B., Fleming, C.C., Szalanski, A.L., Adams, B.A. & Harris, T.S. (1997). The rDNA internal transcribed spacer region as a taxonomic marker for nematodes. Journal of Nematology 29, 441-450.
54. Schmitz, B., Burgermeister, W. & Braasch, H. (1998). Molecular genetic classification of Central European Meloi-dogyne chitwoodi and M. fallax populations. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes 50, 310-317.
55. Schönfeld, U., Braasch, H. & Burgermeister, W. (2006). Bursaphelenchus spp. (Nematoda: Parasitaphelenchi-dae) in wood chips from sawmills in Brandenburg and description of Bursaphelenchus willibaldi sp. n. Russian Journal of Nematology 14, 119-126.
56. Stanton, J.M., McNicol, C.D. & Steele, V. (1998). Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction. Australasian Plant Pathology 27, 112-115.
57. Subbotin, S.A., Halford, P.D., Warry, A. & Perry, R.N. (2000). Variations in ribosomal DNA sequences and phylogeny of Globodera parasitising solanaceous plants. Nematology 2, 591-604.
58. Tomiczek, Ch., Braasch, H., Burgermeister, W., Metge, K., Hoyer, U. & Brandstetter, M. (2003). Bursaphelenchus species found in Chinese packaging wood imported to Austria. Nematology 5, 573-581.
59. Vrain, T.C. (1993). Restriction fragment length polymorphism separates species of the Xiphinema americanum group. Journal of Nematology 25, 361-364.
60. Wang, J.C., Yu, B.Y. & Lin, M.S. (2005). Description of a new species (Nematoda, Aphelenchoididae) isolated from wood packaging material. Acta Zootaxonomica Sinica 30, 314-319.
61. Williams, B.D., Schrank, B., Huynh, C., Shownkeen, R. & Waterston, R.H. (1992). A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131, 609-624.
62. Ye, W., Giblin-Davis , R.M., Braasch, H., Morris, K. & Thomas, W.K. (2007). Phylogenetic relationships among Bursaphelenchus species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution 43, 1185-1197.
63. Zheng, J., Subbotin, S.A., He, S., Gu, J. & Moens, M. (2003). Molecular characterisation of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA. Russian Journal of Nematology 11, 17-22.
64. Zijlstra, C., Lever, A.E.M., Uenk, B.J. & Van Sil-fhout, C.H. (1995). Differences between ITS regions of isolates of root-knot nematodes Meloidogyne hapla and M. chitwoodi. Phytopathology 85, 1231-1237.