Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction

Australasian Plant Pathology

Volumn 27, Issue 2, 1998, Pages 112-115

  Stanton, J M ^a,b   McNicol, C D ^a,b   Steele, V ^a,b  

^a DEPARTMENT OF AGRICULTURE AND FISHERIES   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Diagnostics; Root knot nematodes

Indexed keywords

EID: 0032339149     PISSN: 08153191     EISSN: None     Source Type: Journal    
DOI: 10.1071/AP98014     Document Type: Article

References (7)

1. Harris, T.S., Sandall, L.J. and Powers, T.O. (1990) - Identification of single Meloidogyne juveniles by polymerase chain reaction amplification of mitochondrial DNA. Journal of Nematology 22: 518-524.
2. Hugall, A., Moritz, C., Stanton, J. and Wolstenholme, D. (1994) - Low, but strongly structured mitochondrial DNA diversity in root knot nematodes (Meloidogyne). Genetics 136: 903-912.
3. Klimyuk, V.I., Carroll, B.J., Thomas, C.M. and Jones, J.D.G. (1993) - Alkali treatment for rapid preparation of plant material for reliable PCR analysis. The Plant Journal 3: 493-494.
4. Powers, T.O. and Harris, T.S. (1993) - A polymerase chain reaction method for identification of five major Meloidogyne species. Journal of Nematology 25: 1-6.
5. Sasser, J.N. and Freckman, D.W. (1987) - A world perspective on nematology: the role of the Society In Vistas on Nematology (Eds J.A. Veech and D.W. Dickson), pp. 7-14. Society of Nematologists Inc. Hyattsville.
6. Stanton, J., Hugall, A. and Moritz, C. (1997) - Nucleotide polymorphisms and an improved PCR-based mtDNA diagnostic for parthenogenetic root-knot nematodes (Meloidogyne spp.). Fundamental and Applied Nemotology 20: 261-268.
7. Stanton, J.M., O'Brien, P.C. and Schipke, L.G. (1992) - Species of root-knot nematode (Meloidogyne spp.) affecting tobacco in north Queensland, including two new host races of M. arenaria. Australasian Plant Pathology 21: 150-157.