Klebsiella pneumoniae triggers a cytotoxic effect on airway epithelial cells

BMC Microbiology

Volumn 9, Issue , 2009, Pages

  Cano, Victoria ^a,b   Moranta, David ^a,b   Llobet Brossa, Enrique ^a,b   Bengoechea, José Antonio ^a,b,c,d   Garmendia, Junkal ^a,b,c  

^a Recinto Hospital Joan March   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c UNIVERSITAT DE LES ILLES BALEARS   (Spain)

^d CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; DNA; ETHIDIUM BROMIDE; FORMAZAN; LACTATE DEHYDROGENASE; BACTERIAL POLYSACCHARIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL MEMBRANE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; CONTROLLED STUDY; CYTOTOXICITY; EPITHELIUM CELL; FEMALE; HUMAN; HUMAN CELL; KLEBSIELLA PNEUMONIAE; LUNG ALVEOLUS CELL; MOUSE; NONHUMAN; RESPIRATORY EPITHELIUM; ANIMAL; C57BL MOUSE; CELL LINE; CYTOLOGY; KLEBSIELLA INFECTION; LUNG; MICROBIOLOGY; PATHOGENICITY; PHYSIOLOGY; VIRULENCE;

BACTERIA (MICROORGANISMS); KLEBSIELLA PNEUMONIAE; NEGIBACTERIA;

ANIMALS; BACTERIAL CAPSULES; CELL LINE; EPITHELIAL CELLS; FEMALE; HUMANS; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; LUNG; MICE; MICE, INBRED C57BL; POLYSACCHARIDES, BACTERIAL; VIRULENCE;

EID: 69149106013     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-9-156     Document Type: Article

References (42)

1. Klebsiella pulmonary infections: occurrence at one medical center and review. JL Carpenter, Rev Infect Dis 1990 12 672 682 2201068
2. Hospital-acquired infections in the neonatal intensive care unit-Klebsiella pneumoniae. A Gupta, Semin Perinatol 2002 26 340 345 10.1053/sper.2002.36267 12452506
3. The epidemiology of nosocomial infections caused by Klebsiella pneumoniae. WR Jarvis VP Munn AK Highsmith DH Culver JM Hughes, Infect Control 1985 6 68 74 3882593
4. Bacteriology of hospital-acquired pneumonia. JG Bartlett P O'Keefe FP Tally TJ Louie SL Gorbach, Arch Intern Med 1986 146 868 871 10.1001/archinte. 146.5.868 3516102
5. Production of an extracellular toxic complex by various strains of Klebsiella pneumoniae. DC Straus, Infect Immun 1987 55 44 48 3539806
6. A symphony of bacterial voices [news]. E Strauss, Science 1999 284 1302 1304 10.1126/science.284.5418.1302 10383312
7. Capsular polysaccharide is a major complement resistance factor in lipopolysaccharide O side chain-deficient Klebsiella pneumoniae clinical isolates. D Álvarez S Merino JM Tomás VJ Benedí S Albertí Infect Immun 2000 68 953 955 10.1128/IAI.68.2.953-955.2000 10639470
8. Capsule impedes adhesion to and invasion of epithelial cells by Klebsiella pneumoniae. H Sahly R Podschun TA Oelschlaeger M Greiwe H Parolis D Hasty J Kekow U Ullmann I Ofek S Sela, Infect Immun 2000 68 6744 6749 10.1128/IAI.68.12.6744-6749.2000 11083790
9. Capsule shields the function of short bacterial adhesins. MA Schembri D Dalsgaard P Klemm, J Bacteriol 2004 186 1249 1257 10.1128/JB.186.5.1249-1257. 2004 14973035
10. Capsule and fimbria interaction in Klebsiella pneumoniae. MA Schembri J Blom KA Krogfelt P Klemm, Infect Immun 2005 73 4626 4633 10.1128/IAI.73.8.4626- 4633.2005 16040975
11. Capsule polysaccharide mediates bacterial resistance to antimicrobial peptides. MA Campos MA Vargas V Regueiro CM Llompart S Albertí JA Bengoechea, Infect Immun 2004 72 7107 7114 10.1128/IAI.72.12.7107-7114.2004 15557634
12. Capsule polysaccharide is a bacterial decoy for antimicrobial peptides. E Llobet JM Tomás JA Bengoechea, Microbiology 2008 154 3877 3886 10.1099/mic.0.2008/022301-0 19047754
13. The uptake of a Klebsiella pneumoniae capsule polysaccharide mutant triggers an inflammatory response by human airway epithelial cells. V Regueiro MA Campos J Pons S Albertí JA Bengoechea, Microbiology 2006 152 555 566 10.1099/mic.0.28285-0 16436443
14. Klebsiella pneumoniae increases the levels of Toll-like receptors 2 and 4 in human airway epithelial cells. V Regueiro D Moranta MA Campos J Margareto J Garmendia JA Bengoechea, Infect Immun 2009 77 714 724 10.1128/IAI.00852-08 19015258
15. Role of lung epithelial cells in defense against Klebsiella pneumoniae pneumonia. G Cortés D Álvarez C Saus S Albertí Infect Immun 2002 70 1075 1080 10.1128/IAI.70.3.1075-1080.2002 11854185
16. Molecular analysis of the contribution of the capsular polysaccharide and the lipopolysaccharide O side chain to the virulence of Klebsiella pneumoniae in a murine model of pneumonia. G Cortés N Borrell B de Astorza C Gámez J Sauleda S Albertí Infect Immun 2002 70 2583 2590 10.1128/IAI.70.5.2583-2590.2002 11953399
17. Bacterial lipopolysaccharides extraction with phenol-water and further applications of the procedure. O Westphal K Jann, Meth Carbohydrate Chem 1963 5 83 91
18. Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. M Hirschfeld Y Ma JH Weis SN Vogel JJ Weis, J Immunol 2000 165 618 622 10878331
19. Endotoxin-induced early gene expression in C3H/HeJ (Lpsd) macrophages. CL Manthey PY Perera BE Henricson TA Hamilton N Qureshi SN Vogel, J Immunol 1994 153 2653 2663 7521367
20. A modified uronic acid carbazole reaction. T Bitter HM Muir, Anal Biochem 1962 4 330 334 10.1016/0003-2697(62)90095-7 13971270
21. Transcriptional organization and regulation of the Escherichia coli K30 group 1 capsule biosynthesis (cps) gene cluster. A Rahn C Whitfield, Mol Microbiol 2003 47 1045 1060 10.1046/j.1365-2958.2003.03354.x 12581358
22. Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats. E Díaz-Aparicio V Aragán C Marín B Alonso M Font E Moreno S Pérez-Ortiz JM Blasco R Díaz I Moriyán, J Clin Microbiol 1993 31 3136 3141 8308104
23. Yersinia enterocolitica can deliver Yop proteins into a wide range of cell types: development of a delivery system for heterologous proteins. AP Boyd N Grosdent S Totemeyer C Geuijen S Bleves M Iriarte I Lambermont JN Octave GR Cornelis, Eur J Cell Biol 2000 79 659 671 10.1078/0171-9335-00098 11089914
24. Importance of a lipopolysaccharide-containing extracellular toxic complex in infections produced by Klebsiella pneumoniae. DC Straus DL Atkisson CW Garner, Infect Immun 1985 50 787 795 3905614
25. Enteropathogenic Escherichia coli induces modification of the focal adhesions of infected host cells. Y Shifrin J Kirschner B Geiger I Rosenshine, Cell Microbiol 2002 4 235 243 10.1046/j.1462-5822.2002.00188.x 11952640
26. Escherichia coli cyclomodulin Cif induces G2 arrest of the host cell cycle without activation of the DNA-damage checkpoint-signalling pathway. F Taieb JP Nougayrede C Watrin A Samba-Louaka E Oswald, Cell Microbiol 2006 8 1910 1921 10.1111/j.1462-5822.2006.00757.x 16848790
27. BopB is a type III secreted protein in Bordetella bronchiseptica and is required for cytotoxicity against cultured mammalian cells. A Kuwae M Ohishi M Watanabe M Nagai A Abe, Cell Microbiol 2003 5 973 983 10.1046/j.1462-5822.2003. 00341.x 14641181
28. The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells. SH Shafikhani C Morales J Engel, Cell Microbiol 2008 10 994 1007 10.1111/j.1462-5822.2007.01102.x 18053004
29. Diverse type III secretion phenotypes among Pseudomonas aeruginosa strains upon infection of murine macrophage-like and endothelial cell lines. M Stepinska EA Trafny, Microb Pathog 2008 44 448 458 10.1016/j.micpath.2007.11.008 18221854
30. Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles. JM Bomberger DP Maceachran BA Coutermarsh S Ye GA O'Toole BA Stanton, PLoS Pathog 2009 5 e1000382 10.1371/journal.ppat. 1000382 19360133
31. Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells. NC Kesty KM Mason M Reedy SE Miller MJ Kuehn, EMBO J 2004 23 4538 4549 10.1038/sj.emboj.7600471 15549136
32. Contribution of capsular polysaccharide and surface properties to virulence of Escherichia coli K1. PM Allen I Roberts GJ Boulnois JR Saunders CA Hart, Infect Immun 1987 55 2662 2668 3312006
33. Capsule loss by Haemophilus influenzae type b results in enhanced adherence to and entry into human cells. J St Geme III S Falkow, J Infect Dis 1992 165 S117 S118 1350299
34. Uptake of Streptococcus pneumoniae by respiratory epithelial cells. UM Talbot AW Paton JC Paton, Infect Immun 1996 64 3772 3777 8751928
35. Identification of Klebsiella pneumoniae virulence determinants using an intranasal infection model. MS Lawlor J Hsu PD Rick VL Miller, Mol Microbiol 2005 58 1054 1073 10.1111/j.1365-2958.2005.04918.x 16262790
36. Application of a novel multi-screening signature-tagged mutagenesis assay for identification of Klebsiella pneumoniae genes essential in colonization and infection. C Struve C Forestier KA Krogfelt, Microbiology 2003 149 167 176 10.1099/mic.0.25833-0 12576590
37. Role of capsule in Klebsiella pneumoniae virulence: lack of correlation between in vitro and in vivo studies. C Struve KA Krogfelt, FEMS Microbiol Lett 2003 218 149 154 10.1111/j.1574-6968.2003.tb11511.x 12583911
38. Recognition of bacterial surface polysaccharides by lectins of the innate immune system and its contribution to defense against infection: the case of pulmonary pathogens. H Sahly Y Keisari E Crouch N Sharon I Ofek, Infect Immun 2008 76 1322 1332 10.1128/IAI.00910-07 18086817
39. C3 promotes clearance of Klebsiella pneumoniae by A549 epithelial cells. B de Astorza G Cortés C Crespí C Saus JM Rojo S Albertí Infect Immun 2004 72 1767 1774 10.1128/IAI.72.3.1767-1774.2004 14977986
40. IL-12 gene therapy protects mice in lethal Klebsiella pneumonia. MJ Greenberger SL Kunkel RM Strieter NW Lukacs J Bramson J Gauldie FL Graham M Hitt JM Danforth TJ Standiford, J Immunol 1996 157 3006 3012 8816409
41. Intrapulmonary tumor necrosis factor gene therapy increases bacterial clearance and survival in murine gram-negative pneumonia. TJ Standiford JM Wilkowski TH Sisson N Hattori B Mehrad KA Bucknell TA Moore, Hum Gene Ther 1999 10 899 909 10.1089/10430349950018300 10223724
42. Interleukin-17 and lung host defense against Klebsiella pneumoniae infection. P Ye PB Garvey P Zhang S Nelson G Bagby WR Summer P Schwarzenberger JE Shellito JK Kolls, Am J Respir Cell Mol Biol 2001 25 335 340 11588011