Comparison of full gyrA, gyrB, parC and parE gene sequences between all Ureaplasma parvum and Ureaplasma urealyticum serovars to separate true fluoroquinolone antibiotic resistance mutations from non-resistance polymorphism

Journal of Antimicrobial Chemotherapy

Volumn 64, Issue 3, 2009, Pages 529-538

  Beeton, Michael L ^a   Chalker, Victoria J ^b   Kotecha, Sailesh ^a   Spiller, O Brad ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

Ciprofloxacin; DNA gyrase; Genetic polymorphisms; Topoisomerase IV

Indexed keywords

ALANINE; ASPARTIC ACID; CIPROFLOXACIN; DNA TOPOISOMERASE (ATP HYDROLYSING) A; DNA TOPOISOMERASE (ATP HYDROLYSING) B; GLUTAMINE; MOXIFLOXACIN; OFLOXACIN; PROTEIN PARC; PROTEIN PARE; QUINOLINE DERIVED ANTIINFECTIVE AGENT; THREONINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTIBACTERIAL ACTIVITY; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; CONTROLLED STUDY; GENE CLUSTER; GENE EXPRESSION; GENETIC POLYMORPHISM; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE; UREAPLASMA; UREAPLASMA PARVUM; UREAPLASMA UREALYTICUM;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CLUSTER ANALYSIS; DNA GYRASE; DNA MUTATIONAL ANALYSIS; DNA TOPOISOMERASE IV; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; FLUOROQUINOLONES; GENOTYPE; HUMANS; INFANT, NEWBORN; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; PHYLOGENY; SEQUENCE ALIGNMENT; UNITED STATES; UREAPLASMA; UREAPLASMA INFECTIONS; UREAPLASMA UREALYTICUM;

EID: 69049109923     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkp218     Document Type: Article

References (15)

1. Schelonka RL, Waites KB. Ureaplasma infection and neonatal lung disease. Semin Perinatol 2007; 31: 2-9.
2. Robertson JA, Stemke GW, Davis JW Jr et al. Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum (Shepard et al. 1974) Robertson et al. 2001. Int J Syst Evol Microbiol 2002; 52: 587-97.
3. Waites KB, Crouse DT, Cassell GH. Therapeutic considerations for Ureaplasma urealyticum infections in neonates. Clin Infect Dis 1993; 17 Suppl 1: S208-14.
4. Roberts MC, Kenny GE. Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum. Antimicrob Agents Chemother 1986; 29: 350-2.
5. Beeton ML, Chalker VJ, Maxwell NC et al. Concurrent titration and determination of antibiotic resistance in Ureaplasma species with identification of novel point mutations in genes associated with resistance. Antimicrob Agents Chemother 2009; 53: 2020-7.
6. Bebear CM, Renaudin H, Charron A et al. In vitro activity of trovafloxacin compared to those of five antimicrobials against mycoplasmas including Mycoplasma hominis and Ureaplasma urealyticum fluoroquinolone-resistant isolates that have been genetically characterized. Antimicrob Agents Chemother 2000; 44 2557-60.
7. Bebear CM, Renaudin H, Charron A et al. DNA gyrase and topoisomerase IV mutations in clinical isolates of Ureaplasma spp. and Mycoplasma hominis resistant to fluoroquinolones. Antimicrob Agents Chemother 2003; 47: 3323-5.
8. Duffy L, Glass J, Hall G et al. Fluoroquinolone resistance in Ureaplasma parvum in the United States. J Clin Microbiol 2006; 44: 1590-1.
9. Geissdorfer W, Sandner G, John S et al. Ureaplasma urealyticum meningitis in an adult patient. J Clin Microbiol 2008; 46: 1141-3.
10. Xie X, Zhang J. Trends in the rates of resistance of Ureaplasma urealyticum to antibiotics and identification of the mutation site in the quinolone resistance-determining region in Chinese patients. FEMS Microbiol Lett 2006; 259: 181-6.
11. Zhang W, Wu Y, Yin W et al. Study of isolation of fluoroquinolone-resistant Ureaplasma urealyticum and identification of mutant sites. Chin Med J (Engl) 2002; 115: 1573-5.
12. Tamura K, Dudley J, Nei M et al. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24: 1596-9.
13. Glass JI, Lefkowitz EJ, Glass JS et al. The complete sequence of the mucosal pathogen Ureaplasma urealyticum. Nature 2000; 407 757-62.
14. Piddock LJ. Mechanisms of fluoroquinolone resistance: An update 1994-1998. Drugs 1999; 58 Suppl 2: 11-8.
15. Hooper DC. Mechanisms of fluoroquinolone resistance. Drug Resist Updat 1999; 2: 38-55.