Thioredoxin redox western analysis

Current Protocols in Toxicology

Volumn , Issue SUPPL. 41, 2009, Pages

  Go, Young Mi ^a   Jones, Dean P ^a  

^a EMORY UNIVERSITY   (United States)

Author keywords

Cysteine; Redox potential; Redox state; Thiol disulfide

Indexed keywords

BUFFER; CYSTEINE; DIAMIDE; DITHIOTHREITOL; GLUTATHIONE; GUANIDINE; HYDROGEN PEROXIDE; POLYACRYLAMIDE; TERT BUTYL HYDROPEROXIDE; THIOREDOXIN 1; THIOREDOXIN 2;

ANALYTIC METHOD; CALCULATION; CELL CULTURE; DERIVATIZATION; HUMAN; HUMAN CELL; MEASUREMENT; OXIDATION REDUCTION POTENTIAL; OXIDATION REDUCTION REACTION; OXIDATION REDUCTION STATE; PRIORITY JOURNAL; REDOX WESTERN ANALYSIS; REVIEW; SAMPLING; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

CELL NUCLEUS; CYSTEINE; CYTOPLASM; HELA CELLS; HUMANS; MITOCHONDRIA; OXIDATION-REDUCTION; SIGNAL TRANSDUCTION; THIOREDOXINS;

EID: 68949155324     PISSN: 19349254     EISSN: 19349262     Source Type: Journal    
DOI: 10.1002/0471140856.tx1712s41     Document Type: Review

References (16)

1. Chen, Y., Cai, J., Murphy, T.J., and Jones, D.P. 2002. Overexpressed human mitochondrial thioredoxin confers resistance to oxidant-induced apoptosis in human osteosarcoma cells. J. Biol. Chem. 277:33242-33248.
2. Chen, Y., Cai, J., and Jones, D.P. 2006. Mitochondrial thioredoxin in regulation of oxidantinduced cell death. FEBS Lett. 580:6596-6602.
3. Fernando, M.R., Nanri, H., Yoshitake, S., Nagata-Kuno, K., and Minakami, S. 1992. Thioredoxin regenerates proteins inactivated by oxidative stress in endothelial cells. Eur. J. Biochem. 209:917-922.
4. Go, Y.M. and Jones, D.P. 2008. Redox compartmentalization in eukaryotic cells. Biochim. Biophys. Acta 1780:1273-1290.
5. Go, Y.M., Ziegler, T.R., Johnson, J.M., Gu, L., Hansen, J.M., and Jones, D.P. 2007. Selective protection of nuclear thioredoxin-1 and glutathione redox systems against oxidation during glucose and glutamine deficiency in human colonic epithelial cells. Free Radic. Biol. Med. 42:363-370.
6. Gutscher, M., Pauleau, A.L., Marty, L., Brach, T., Wabnitz, G.H., Samstag, Y., Meyer, A.J., and Dick, T.P. 2008. Real-time imaging of the intracellular glutathione redox potential. Nat. Methods 5:553-559.
7. Halvey, P.J., Watson, H., Hansen, J.M., Go, Y.M., Samali, A., and Jones, D.P. 2005. Compartmental oxidation of thiol-disulphide redox couples during epidermal growth factor signaling. Biochem. J. 386:215-219.
8. Hansen, J.M., Zhang, H., and Jones, D.P. 2006. Mitochondrial thioredoxin-2 has a key role in determining tumor necrosis factor-alpha-induced reactive oxygen species generation, NF-kappaB activation, and apoptosis. Toxicol. Sci. 91:643-650.
9. Hirota, K., Matsui, M., Iwata, S., Nishiyama, A., Mori, K., and Yodoi, J. 1997. AP-1 transcriptional activity is regulated by a direct association between thioredoxin and Ref-1. Proc. Natl. Acad. Sci. U.S.A. 94:3633-3638.
10. Holmgren, A. and Fagerstedt, M. 1982. The in vivo distribution of oxidized and reduced thioredoxin in Escherichia coli. J. Biol. Chem. 257:6926-6930.
11. Jones, D.P. 2008. Radical-free biology of oxidative stress. Amer. J. Physiol. 295:C849-C868.
12. Kim, J.R., Lee, S.M., Cho, S.H., Kim, J.H., Kim, B.H., Kwon, J., Choi, C.Y., Kim, Y.D., and Lee, S.R. 2004. Oxidation of thioredoxin reductase in HeLa cells stimulated with tumor necrosis factor-alpha. FEBS Lett. 567:189-196.
13. Kim, Y.J., Lee, W.S., Ip, C., Chae, H.Z., Park, E.M., and Park, Y.M. 2006. Prx1 suppresses radiation-induced c-Jun NH2-terminal kinase signaling in lung cancer cells through interaction with the glutathione S-transferase Pi/c-Jun NH2-terminal kinase complex. Cancer Res. 66:7136-7142.
14. Leichert, L.I., Gehrke, F., Gudiseva, H.V., Blackwell, T., Ilbert, M., Walker, A.K., Strahler, J.R., Andrews, P.C., and Jakob, U. 2008. Quantifying changes in the thiol redox proteome upon oxidative stress in vivo. Proc. Natl. Acad. Sci. U.S.A. 105:8197-8205.
15. Saitoh, M., Nishitoh, H., Fujii, M., Takeda, K., Tobiume, K., Sawada, Y., Kawabata, M., Miyazono, K., and Ichijo, H. 1998. Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1. EMBO J. 17:2596-2606.
16. Watson, W.H., Pohl, J., Montford, W.R., Stuchlik, O., Reed, M.S., Powis, G., and Jones, D.P. 2003. Redox potential of human thioredoxin 1 and identification of a second dithiol/disulfide motif. J. Biol. Chem. 278:33408-33415.