Cytoplasmic expression of recombinant interleukin-2 and interleukin-4 proteins results in hydrogen peroxide accumulation and reduction in catalase activity in Escherichia coli

Daru

Volumn 17, Issue 2, 2009, Pages 64-71

  Hejazi, M S ^a,b   Karimi, F ^c,d   Mehdizadeh Aghdam, E ^a   Barzegari, A ^e   Farshdosti Hagh, M ^a   Parvizi, M ^a   Mahmoodi Azar, L ^a,f   Hejazi, M A ^e  

^a TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^c UNIVERSITY OF MARAGHEH   (Iran)

^d TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^e AGRICULTURAL BIOTECHNOLOGY RESEARCH INSTITUTE OF IRAN ABRII   (Iran)

^f ISLAMIC AZAD UNIVERSITY   (Iran)

Author keywords

Catalase activity; Cytoplasmic expression; Escherichia coli; Hydrogen peroxide; Recombinant protein

Indexed keywords

CATALASE; DNA; HYDROGEN PEROXIDE; PLASMID VECTOR; RECOMBINANT INTERLEUKIN 2; RECOMBINANT INTERLEUKIN 4;

ARTICLE; CELL GROWTH; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE AMPLIFICATION; GROWTH CURVE; PROTEIN EXPRESSION;

EID: 68949136469     PISSN: 15608115     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Diaz Ricci JC, Hernández ME. Plasmid effects on Escherichia coli metabolism. Crit Rev Biotechnol 2000; 20: 79-108.
2. Siak-Wei Ow D, Morin Nissom P, Phlip R, Kah-Weng Oh S, Gek-Sim Yap M. Global transcriptional analysis of metabolic burden due to plasmid maintenance in Escherichia coli DH5 during batch fermentation. Enzym Microb Tech 2006; 39: 391-398.
3. Bentley WE, Mirjalili N, Anderson DC, Davis RH, Kompala DC. Plasmid-encoded protein: The principal factor in the "metabolic burden" associated with recombinant bacteria. Biotechnol Bioeng 1990; 35: 668-681.
4. Glick BR. Metabolic load and heterologous gene expression. Biotechnol Adv 1995; 13: 247-261.
5. Rozkov A, Avignone-Rossa CA, Ertl PF, Jones P, O'Kennedy RD, Smith JJ, Dale JW, Bushell ME. Characterization of the Metabolic burden on Escherichia coli DH1 cells imposed by the presence of a plasmid containing a gene therapy sequence. Biotechnol Bioeng 2004; 88: 909-915.
6. Hoffmann F, Rinas U. Stress induced by recombinant protein production in Escherichia coli. Adv Biochem Engin/Biotechnol 2004; 89: 73-92.
7. Birnbaum S, Bailey JE. Plasmid presence changes the relative levels of many host cell proteins and ribosome components in recombinant Escherichia coli. Biotechnol Bioeng 1991; 37: 736-745.
8. Anderson L, Yang S, Neubauer P, Enfors SO. Impact of plasmid presence on cellular responses in fed batch cultures of Escherichia coli. J Biotechnol 1996; 46: 255-263.
9. Hoffmann F, Rinas U. On-line estimation of the metabolic burden resulting from synthesis of plasmid-encoded and heat-shock proteins by monitoring respiratory energy generation. Biotechnol Bioeng 2001; 76: 333-340. (Pubitemid 34015023)
10. Summers DK. The kinetics of plasmid loss. Trends Biotechnol 1991; 9: 273-278.
11. Kumar PKR, Maschke HE, Friehs K, Schügerl K. Strategies for improving plasmid stability in genetically modified bacteria in bioreactors. Trends Biotechnol 1991; 9: 279-284. (Pubitemid 121003122)
12. Kaprálek F, Ječmen P. The structural stability of an expression plasmid bearing a heterologous cloned gene depends on whether this gene is expressed or not. Biotechnol Lett 1992; 14: 251-256.
13. Storz G, Imlay JA. Oxidative stress. Curr Opin Microbiol 1999; 2: 188-194.
14. Aertsen A, De Spiegeleer P, Vanoirbeek K, Lavilla M, W. Michiels C. Induction of oxidative stress by high hydrostatic pressure in Escherichia coli. Appl Environ Microbiol 2005; 71: 2226-2231.
15. Galli F, Piroddi M, Annetti C, Aisa C, Floridi E, Floridi A. Oxidative stress and reactive oxygen species. Contrib Nephrol 2005; 149: 240-260 (Pubitemid 43265021)
16. Cabiscol E,Tamarit J, Ros J. Oxidative stress in bacteria and protein damage by reactive oxygen species. Internatl Microbiol 2000; 3: 3-8.
17. Lushchak VI. Oxidative stress and mechanisms of protection against it in bacteria. Biochemistry(Moscow) 2001; 66: 476-489.
18. Semchyshyn HM, Lushchak VI. Oxidative stress and control of catalase activity in Escherichia coli. Ukr Biokhim Zh 2004; 76: 31-42.
19. Iuchi S, Weiner L. Cellular and molecular physiology of Escherichia coli in the adaptation to aerobic environments. J Biochem 1996; 120: 1055-1063.
20. Tabor S, Richardson CC. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 1985; 82: 1074-1078.
21. Studier FW, Moffat BA. Use of Bacteriophage T7 RNA polymerase to direct selective high-level expression of coloned genes. J Mol Biol 1986; 189: 113-130.
22. Hejazi MS, Pournaghi-Azar MH, Alipour E, Karimi F. Construction, electrochemically biosensing and discrimination of recombinant plasmid (pEThIL-2) on the basis of interleukine-2 DNA insert. Biosensors and Bioelectronics 2008; 23: 1588-1594.
23. Sambrook J, Rucell D. Molecular Cloning. A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, Cold Spring Harbor; 2001.
24. Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid raintreated bean plants- Protective role of exogenous polyamines. Plant Sci 2000; 151: 59-66. (Pubitemid 30046561)
25. Lushchak V, Semchyshyn H, Storey K. Possible reasons for difference in sensitivity to oxygen of two Escherichia coli strains. Biochemistry (Moscow) 2005; 70: 424-431.
26. Bradford MM. A rapid and sensitive method for quantitation of protein microgram quantities of protein utilizing principle of protein-dye binding. Anal Biochem 1976; 72: 248-256
27. Lushchak V, Lushchak L, Mota A, Hermes-Lihma M. Oxidative stress and antioxidant defenses in goldfish Carassius auratus during anoxia and reoxygenation. Am J Physiol 2001; 280: R100-R107.