Genistein inhibition of topoisomerase IIα expression participated by Sp1 and Sp3 in HeLa cell

International Journal of Molecular Sciences

Volumn 10, Issue 7, 2009, Pages 3255-3268

  Zhou, Najing ^a   Yan, Yunli ^a   Li, Wenling ^a   Wang, Yanling ^a   Zheng, Lifen ^a   Han, Shuo ^a   Yan, Yongxin ^a   Li, Yunzhi ^b  

^a HEBEI MEDICAL UNIVERSITY   (China)

^b Chang'an District Hospital   (China)

Author keywords

Genistein; Specificity protein 1 (Sp1); Specificity protein 3 (Sp3); Topoisomerase II

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; DNA TOPOISOMERASE (ATP HYDROLYSING); GENISTEIN; LIPOCORTIN 5; PROPIDIUM IODIDE; TOPOISOMERASE II ALPHA; TRANSCRIPTION FACTOR SP1; TRANSCRIPTION FACTOR SP3; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL CYCLE PARAMETERS; CELL CYCLE PHASE; CELL PROLIFERATION; CONTROLLED STUDY; DNA REPLICATION; FLOW CYTOMETRY; GENE EXPRESSION; HELA CELL; HUMAN; HUMAN CELL; MICROMORPHOLOGY; PROMOTER REGION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

GENISTEIN; SPECIFICITY PROTEIN 1 (SP1); SPECIFICITY PROTEIN 3 (SP3); TOPOISOMERASE II-Α;

ANTICARCINOGENIC AGENTS; ANTIGENS, NEOPLASM; APOPTOSIS; DNA TOPOISOMERASES, TYPE II; DNA-BINDING PROTEINS; GENISTEIN; HELA CELLS; HUMANS; SP1 TRANSCRIPTION FACTOR; SP3 TRANSCRIPTION FACTOR; TRANSCRIPTION, GENETIC;

EID: 68949101231     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms10073255     Document Type: Article

References (29)

1. Lee, Y.W.; Lee, W.H. Protective effects of genistein on proinflammatory pathways in human brain microvascular endothelial cells. J. Nutr. Biochem. 2008, 19, 819-825.
2. Okamura, S.; Sawada, Y.; Satoh, T.; Sakamoto, H.; Saito, Y.; Sumino, H.; Takizawa, T.; Kogure, T.; Chaichantipyuth, C.; Higuchi, Y.; Ishikawa, T., Sakamaki, T. Pueraria mirifica phytoestrogens improve dyslipidemia in postmenopausal women probably by activating estrogen receptor subtypes. Tohoku J. Exp. Med. 2008, 216, 341-351.
3. Davis, T.A.; Mungunsukh, O.; Zins, S.; Day, R.M.; Landauer, M.R. Genistein induces radioprotection by hematopoietic stem cell quiescence. Int. J. Radiat. Biol. 2008, 84, 713-726.
4. Mandraju, R.K.; Kondapi, A.K. Regulation of topoisomerase II alpha and beta in HIV-1 infected and uninfected neuroblastoma and astrocytoma cells: involvement of distinct nordihydroguaretic acid sensitive inflammatory pathways. Arch. Biochem. Biophys. 2007, 461, 40-49.
5. Mandraju, R.K.; Kannapiran, P.; Kondapi, A.K. Distinct roles of Topoisomerase II isoforms: DNA damage accelerating alpha, double strand break repair promoting beta. Arch. Biochem. Biophys. 2008, 470, 27-34.
6. Katz, J.; Blake, E.; Medrano, T.A.; Sun, Y.; Shiverick, K.T. Isoflavones and gamma irradiation inhibit cell growth in human salivary gland cells. Cancer Lett. 2008, 270, 87-94.
7. Schmidt, F.; Knobbe, C.B.; Frank, B.; Wolburg, H.; Weller, M. The topoisomerase II inhibitor, genistein, induces G2/M arrest and apoptosis in human malignant glioma cell lines. Oncol. Rep. 2008, 19, 1061-1066.
8. Chodon, D.; Ramamurty, N.; Sakthisekaran, D. Preliminary studies on induction of apoptosis by genistein on HepG2 cell line. Toxicol. in Vitro 2007, 21, 887-891.
9. Privat, M.; Aubel, C.; Arnould, S.; Communal, Y.; Ferrara, M.; Bignon, Y.J. Breast cancer cell response to genistein is conditioned by BRCA1 mutations. Biochem. Biophys. Res. Commun. 2009, 379, 785-789.
10. Bandele, O.J.; Osheroff, N. Bioflavonoids as poisons of human topoisomerase II alpha and II beta. Biochemistry 2007, 46, 6097-6108.
11. Michael McClain, R.; Wolz, E.; Davidovich, A.; Bausch, J. Genetic toxicity studies with genistein. Food Chem. Toxicol. 2006, 44, 42-55.
12. Kaufmann, S.H. Cell death induced by topoisomerase-targeted drugs: more questions than answers. Biochim. Biophys. Acta 1998, 1400, 195-211.
13. Markovits, J.; Linassier, C.; Fosse, P.; Couprie, J.; Pierre, J.; Jacquemin-Sablon, A.; Saucier, J.M.; Le Pecq, J.B.; Larsen, A.K. Inhibitory effects of the tyrosine kinase inhibitor genistein on mammalian DNA topoisomerase II. Cancer Res. 1989, 49, 5111-5117.
14. Notarnicola, M.; Messa, C.; Orlando, A.; D'Attoma, B.; Tutino, V.; Rivizzigno, R.; Caruso, M.G. Effect of genistein on cholesterol metabolism-related genes in a colon cancer cell line. Genes Nutr. 2008, 3, 35-40.
15. Akiyama, T.; Ishida, J.; Nakagawa, S.; Ogawara, H.; Watanabe, S.; Itoh, N.; Shibuya, M.; Fukami, Y. Genistein, a specific inhibitor of tyrosine-specific protein kinases. J. Biol. Chem. 1987, 262, 5592-5595.
16. Zou, H.; Zhan, S.; Cao, K. Apoptotic activity of genistein on human lung adenocarcinoma SPC-A-1 cells and preliminary exploration of its mechanisms using microarray. Biomed. Pharmacother. 2008, 62, 583-589.
17. Yamaguchi, M.; Weitzmann, M.N. The estrogen 17beta-estradiol and phytoestrogen genistein mediate differential effects on osteoblastic NF-kappaB activity. Int. J. Mol. Med. 2009, 23, 297-301.
18. Ismail, I.A.; Kang, K.S.; Lee, H.A.; Kim, J.W.; Sohn, Y.K. Genistein-induced neuronal apoptosis and G2/M cell cycle arrest is associated with MDC1 up-regulation and PLK1 down-regulation. Eur. J. Pharmacol. 2007, 575, 12-20.
19. Caruso, M.G.; Messa, C.; Orlando, A.; D'Attoma, B.; Notarnicola, M. Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line. Fitoterapia 2008, 79, 524-528.
20. Banerjee, S.; Li, Y.; Wang, Z.; Sarkar, F.H. Multi-targeted therapy of cancer by genistein. Cancer Lett. 2008, 269, 226-242.
21. Rucinska, A.; Roszczyk, M.; Gabryelak, T. Cytotoxicity of the isoflavone genistein in NIH 3T3 cells. Cell Biol. Int. 2008, 32, 1019-1023.
22. Hochhauser, D.; Stanway, C.A.; Harris, A.L.; Hickson, I.D. Cloning and characterization of the 5′-flanking region of the human topoisomerase II alpha gene. J. Biol. Chem. 1992, 267, 18961-18965.
23. Suske, G. The Sp-family of transcription factors. Gene 1999, 238, 291-300.
24. Magan, N.; Szremska, A.P.; Isaacs, R.J.; Stowell, K.M. Modulation of DNA topoisomerase II alpha promoter activity by members of the Sp (specificity protein) and NF-Y (nuclear factor Y) families of transcription factors. Biochem. J. 2003, 374, 723-729.
25. Lok, C.N.; Lang, A.J.; Mirski, S.E.; Cole, S.P. Characterization of the human topoisomerase IIbeta (TOP2B) promoter activity: essential roles of the nuclear factor-Y (NF-Y)- and specificity protein-1 (Sp1)-binding sites. Biochem. J. 2002, 368, 741-751.
26. Xu, R.; Zhang, P.; Huang, J.; Ge, S.; Lu, J.; Qian, G. Sp1 and Sp3 regulate basal transcription of the survivin gene. Biochem. Biophys. Res. Commun. 2007, 356, 286-292.
27. Williams, A.O.; Isaacs, R.J.; Stowell, K.M. Down-regulation of human topoisomerase IIalpha expression correlates with relative amounts of specificity factors Sp1 and Sp3 bound at proximal and distal promoter regions. BMC Mol. Biol. 2007, 8, 36.
28. Hua, P.; Tsai, W.J.; Kuo, S.M. Estrogen response element-independent regulation of gene expression by genistein in intestinal cells. Biochim. Biophys. Acta 2003, 1627, 63-70.
29. Maeno, T.; Tanaka, T.; Sando, Y.; Suga, T.; Maeno, Y.; Nakagawa, J.; Hosono, T.; Sato, M.; Akiyama, H.; Kishi, S.; Nagai, R.; Kurabayashi, M. Stimulation of vascular endothelial growth factor gene transcription by all trans retinoic acid through Sp1 and Sp3 sites in human bronchioloalveolar carcinoma cells. Am. J. Respir. Cell Mol. Biol. 2002, 26, 246-253.