Alternating-site mechanism of kinesin-1 characterized by single-molecule FRET using fluorescent ATP analogues

Biophysical Journal

Volumn 97, Issue 1, 2009, Pages 173-182

  Verbrugge, Sander ^a   Lechner, Bettina ^b,c   Woehlke, Günther ^b,c   Peterman, Erwin J G ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; FLUORESCENT DYE; KINESIN 1;

ARTICLE; CONFOCAL MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROLYSIS KINETICS; MATHEMATICAL MODEL; MONTE CARLO METHOD; NUCLEOTIDE BINDING SITE; NUCLEOTIDE TRANSPORT;

ADENOSINE TRIPHOSPHATE; COMPUTER SIMULATION; CYCLIC AMP; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; KINESIN; KINETICS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MODELS, CHEMICAL; MONTE CARLO METHOD; MOTION; NUCLEOTIDES; TIME FACTORS;

EID: 68949083836     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2009.02.073     Document Type: Article

References (27)

1. Woehlke, G., and M. Schliwa. 2000. Walking on two heads: the many talents of kinesin. Nat. Rev. Mol. Cell Biol. 1:50-58.
2. Carter, N. J., and R. A. Cross. 2005. Mechanics of the kinesin step. Nature. 435:308-312.
3. Crevel, I. M., M. Nyitrai, M. C. Alonso, S. Weiss, M. A. Geeves, et al. 2004. What kinesin does at roadblocks: the coordination mechanism for molecular walking. EMBO J. 23:23-32.
4. Asenjo, A. B., N. Krohn, and H. Sosa. 2003. Configuration of the two kinesin motor domains during ATP hydrolysis. Nat. Struct. Biol. 10:836-842.
5. Guydosh, N. R., and S. M. Block. 2006. Backsteps induced by nucleotide analogs suggest the front head of kinesin is gated by strain. Proc. Natl. Acad. Sci. USA. 103:8054-8059.
6. Hackney, D. D. 2005. The tethered motor domain of a kinesin-microtubule complex catalyzes reversible synthesis of bound ATP. Proc. Natl. Acad. Sci. USA. 102:18338-18343.
7. Hackney, D. D. 2007. Biochemistry. Processive motor movement. Science. 316:58-59.
8. Mori, T., R. D. Vale, and M. Tomishige. 2007. How kinesin waits between steps. Nature. 450:750-755.
9. Skiniotis, G., T. Surrey, S. Altmann, H. Gross, Y. H. Song, et al. 2003. Nucleotide-induced conformations in the neck region of dimeric kinesin. EMBO J. 22:1518-1528.
10. Yildiz, A., M. Tomishige, A. Gennerich, and R. D. Vale. 2008. Intramolecular strain coordinates kinesin stepping behavior along microtubules. Cell. 134:1030-1041.
11. Alonso, M. C., D. R. Drummond, S. Kain, J. Hoeng, L. Amos, et al. 2007. An ATP gate controls tubulin binding by the tethered head of kinesin-1. Science. 316:120-123.
12. Gilbert, S. P., and K. A. Johnson. 1994. Pre-steady-state kinetics of the microtubule-kinesin ATPase. Biochemistry. 33:1951-1960.
13. Hackney, D. D. 1994. Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis. Proc. Natl. Acad. Sci. USA. 91:6865-6869.
14. Ma, Y. Z., and E. W. Taylor. 1997. Interacting head mechanism of microtubule-kinesin ATPase. J. Biol. Chem. 272:724-730.
15. Verbrugge, S., L. C. Kapitein, and E. J. G. Peterman. 2007. Kinesin moving through the spotlight: single-motor fluorescence microscopy with submillisecond time resolution. Biophys. J. 92:2536-2545.
16. Tomishige, M., and R. D. Vale. 2000. Controlling kinesin by reversible disulfide cross-linking: identifying the motility-producing conformational change. J. Cell Biol. 151:1081-1092.
17. Rasnik, I., S. A. McKinney, and T. Ha. 2006. Nonblinking and longlasting single-molecule fluorescence imaging. Nat. Methods. 3: 891-893.
18. Magde, D., W. W. Webb, and E. Elson. 1972. Thermodynamic fluctuations in a reacting system-measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29:705-708.
19. Torres, T., and M. Levitus. 2007. Measuring conformational dynamics: a new FCS-FRET approach. J. Phys. Chem. B. 111:7392-7400.
20. Haustein, E., and P. Schwille. 2003. Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy. Methods. 29:153-166.
21. Block, S. M., C. L. Asbury, J. W. Shaevitz, and M. J. Lang. 2003. Probing the kinesin reaction cycle with a 2D optical force clamp. Proc. Natl. Acad. Sci. USA. 100:2351-2356.
22. Cross, R. A. 2004. The kinetic mechanism of kinesin. Trends Biochem. Sci. 29:301-309.
23. Schief, V. R., and O. Howard. 2001. Conformational changes during kinesin motility. Curr. Opin. Cell Biol. 13:19-28.
24. Asbury, C. L., A. N. Fehr, and S. M. Block. 2003. Kinesin moves by an asymmetric hand-over-hand mechanism. Science. 302:2130-2134.
25. Kaseda, K., H. Higuchi, and K. Hirose. 2003. Alternate fast and slow stepping of a heterodimeric kinesin molecule. Nat. Cell Biol. 5:1079-1082.
26. Yildiz, A., M. Tomishige, R. D. Vale, and P. R. Selvin. 2004. Kinesin walks hand-over-hand. Science. 303:676-678.
27. Sack, S., J. Muller, A. Marx, M. Thormahlen, E. M. Mandelkow, et al. 1997. X-ray structure of motor and neck domains from rat brain kinesin. Biochemistry. 36:16155-16165.