Identification and characterization of a complete carnitine biosynthesis pathway in Candida albicans

FASEB Journal

Volumn 23, Issue 8, 2009, Pages 2349-2359

  Strijbis, Karin ^a   Van Roermund, Carlo W T ^a   Hardy, Guy P ^a   Van Den Burg, Janny ^a   Bloem, Karien ^a   De Haan, Jolanda ^a   Van Vlies, Naomi ^a   Wanders, Ronald J A ^a   Vaz, Frédéric M ^a   Distel, Ben ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Acetyl unit transport; Carnitine acetyltransferase; Hydroxytrimethyllysine aldolase; Nonfermentable carbon source; Threonine aldolase

Indexed keywords

ACETIC ACID; ALCOHOL; BUTYROBETAINE DIOXYGENASE; CARNITINE; FATTY ACID; FUNGAL ENZYME; HYDROXYTRIMETHYLLYSINE ALDOLASE; OXIDOREDUCTASE; TRIMETHYLAMINOBUTYRALDEHYDE DEHYDROGENASE; TRIMETHYLLYSINE DIOXYGENASE; UNCLASSIFIED DRUG; 4 N TRIMETHYLAMINOBUTYRALDEHYDE DEHYDROGENASE; 4-N-TRIMETHYLAMINOBUTYRALDEHYDE DEHYDROGENASE; 6 N TRIMETHYLLYSINE HYDROXYLASE; ALDEHYDE DEHYDROGENASE; BETA HYDROXY TRIMETHYLLYSINE ALDOLASE; BETA-HYDROXY-TRIMETHYLLYSINE ALDOLASE; EPSILON-N-TRIMETHYLLYSINE HYDROXYLASE; GAMMA BUTYROBETAINE DIOXYGENASE; LYASE; MIXED FUNCTION OXIDASE;

ARTICLE; BIOSYNTHESIS; CANDIDA ALBICANS; CARBON SOURCE; CONTROLLED STUDY; ENZYME ACTIVITY; FUNGAL GENE; FUNGAL STRAIN; FUNGUS GROWTH; FUNGUS MUTANT; GENE IDENTIFICATION; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; AMINO ACID SEQUENCE; BIOLOGICAL MODEL; CHEMISTRY; GENETICS; GROWTH, DEVELOPMENT AND AGING; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; SEQUENCE HOMOLOGY;

CANDIDA ALBICANS;

ALDEHYDE OXIDOREDUCTASES; ALDEHYDE-LYASES; AMINO ACID SEQUENCE; CANDIDA ALBICANS; CARNITINE; GAMMA-BUTYROBETAINE DIOXYGENASE; GENES, FUNGAL; KINETICS; MIXED FUNCTION OXYGENASES; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MUTATION; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 68849090311     PISSN: 08926638     EISSN: 15306860     Source Type: Journal    
DOI: 10.1096/fj.08-127985     Document Type: Article

References (41)

1. Bremer, J. (1983) Carnitine-metabolism and functions. Physiol. Rev. 63, 1420-1480
2. Rebouche, C. J., and Engel, A. G. (1984) Kinetic compartmental analysis of carnitine metabolism in the human carnitine deficiency syndromes. Evidence for alterations in tissue carnitine transport. J. Clin. Invest. 73, 857-867
3. Kaufman, R. A., and Broquist, H. P. (1977) Biosynthesis of carnitine in Neurospora crassa. J. Biol. Chem. 252, 7437-7439
4. Bremer, J. (1962) Carnitine precursors in the rat. Biochim. Biophys. Acta 57, 327-335
5. Tanphaichitr, V., and Broquist, H. P. (1973) Role of lysine and -N-trimethyllysine in carnitine biosynthesis. II. Studies in the rat. J. Biol. Chem. 248, 2176-2181
6. Hochalter, J. B., and Henderson, L. M. (1976) Carnitine biosynthesis: the formation of glycine from carbons 1 and 2 of 6-N-trimethyl-L-lysine. Biochem. Biophys. Res. Commun. 70, 364-368
7. Hulse, J. D., Ellis, S. R., and Henderson, L. M. (1978) Carnitine biosynthesis. beta-Hydroxylation of trimethyllysine by an alpha-ketoglutarate- dependent mitochondrial dioxygenase. J. Biol. Chem. 253, 1654-1659
8. Paik, W. K., and Kim, S. (1971) Protein methylation. Science 174, 114-119
9. Vaz, F. M., and Wanders, R. J. (2002) Carnitine biosynthesis in mammals. Biochem. J. 361, 417-429
10. Vaz, F. M., Ofman, R., Westinga, K., Back, J. W., and Wanders, R. J. (2001) Molecular and biochemical characterization of rat epsilon-N- trimethyllysine hydroxylase, the first enzyme of carnitine biosynthesis. J. Biol. Chem. 276, 33512-33517 (Pubitemid 37384562)
11. Vaz, F. M., van Gool, S., Ofman, R., Ijlst, L., and Wanders, R. J. (1998) Carnitine biosynthesis: identification of the cDNA encoding human gamma-butyrobetaine hydroxylase. Biochem. Biophys. Res. Commun. 250, 506-510 (Pubitemid 28463910)
12. Vaz, F. M., Fouchier, S. W., Ofman, R., Sommer, M., and Wanders, R. J. (2000) Molecular and biochemical characterization of rat gamma- trimethylaminobutyraldehyde dehydrogenase and evidence for the involvement of human aldehyde dehydrogenase 9 in carnitine biosynthesis. J. Biol. Chem. 275, 7390-7394 (Pubitemid 30146293)
13. Henderson, L. M., Hulse, J. D., and Henderson, L. L. (1980) Purification of the enzymes involved in the conversion of trimethyl-lysine to trimethylaminobutyrate. In Carnitine Biosynthesis, Metabolism and Functions (Frenkel, R. A., and McGarry, J. D., eds) pp. 35-43, Academic Press, New York
14. Henderson, L. M., Nelson, P. J., and Henderson, L. (1982) Mammalian enzymes of trimethyllysine conversion to trimethylaminobutyrate. Fed. Proc. 41, 2843-2847
15. Ogawa, H., and Fujioka, M. (1981) Purification and characterization of cytosolic and mitochondrial serine hydroxymethyltransferases from rat liver. J. Biochem. 90, 381-390 (Pubitemid 11012618)
16. Dunn, W. A., Aronson, N. N. Jr., and Englard, S. (1982) The effects of 1-amino-D-proline on the production of carnitine from exogenous protein-bound trimethyllysine by the perfused rat liver. J. Biol. Chem. 257, 7948-7951
17. Ogawa, A., Sawada, K., Saito, K., Hakamada, Y., Sumitomo, N., Hatada, Y., Kobayashi, T., and Ito, S. (2000) A new high-alkaline and high-molecular-weight pectate lyase from a Bacillus isolate: enzymatic properties and cloning of the gene for the enzyme. Biosci. Biotechnol. Biochem. 64, 1133-1141
18. Ogawa, H., Gomi, T., and Fujioka, M. (2000) Serine hydroxymethyltransferase and threonine aldolase: are they identical? Int. J. Biochem. Cell Biol. 32, 289-301 (Pubitemid 30069971)
19. Monschau, N., Stahmann, K. P., Sahm, H., McNeil, J. B., and Bognar, A. L. (1997) Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis. FEMS Microbiol. Lett. 150, 55-60 (Pubitemid 27234412)
20. Swiegers, J. H., Dippenaar, N., Pretorius, I. S., and Bauer, F. F. (2001) Carnitine-dependent metabolic activities in Saccharomyces cerevisiae: three carnitine acetyltransferases are essential in a carnitine-dependent strain. Yeast 18, 585-595 (Pubitemid 32496335)
21. van Roermund, C. W., Hettema, E. H., van den Berg, M., Tabak, H. F., and Wanders, R. J. (1999) Molecular characterization of carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria in Saccharomyces cerevisiae and identification of a plasma membrane carnitine transporter, Agp2p. EMBO J. 18, 5843-5852
22. Staib, P., Wirsching, S., Strauss, A., and Morschhauser, J. (2001) Gene regulation and host adaptation mechanisms in Candida albicans. Int. J. Med. Microbiol. 291, 183-188
23. Strijbis, K., van Roermund, C. W., Visser, W. F., Mol, E. C., van den Burg, J., MacCallum, D. M., Odds, F. C., Paramonova, E., Krom, B. P., and Distel, B. (2008) Carnitine-dependent transport of acetyl coenzyme A in Candida albicans is essential for growth on nonfermentable carbon sources and contributes to biofilm formation. Eukaryot. Cell 7, 610-618 (Pubitemid 351959895)
24. Zhou, H., and Lorenz, M. C. (2008) Carnitine acetyltransferases are required for growth on non-fermentable carbon sources but not for pathogenesis in Candida albicans. Microbiology 154, 500-509 (Pubitemid 351292304)
25. Noble, S. M., and Johnson, A. D. (2005) Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans. Eukaryot. Cell 4, 298-309 (Pubitemid 40257130)
26. Gola, S., Martin, R., Walther, A., Dunkler, A., and Wendland, J. (2003) New modules for PCR-based gene targeting in Candida albicans: rapid and efficient gene targeting using 100 bp of flanking homology region. Yeast 20, 1339-1347 (Pubitemid 38016628)
27. Schaub, Y., Dunkler, A., Walther, A., and Wendland, J. (2006) New pFA-cassettes for PCR-based gene manipulation in Candida albicans. J. Basic Microbiol. 46, 416-429 (Pubitemid 44582323)
28. Boeke, J. D., Trueheart, J., Natsoulis, G., and Fink, G. R. (1987) 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 154, 164-175
29. Ramon, A. M., and Fonzi, W. A. (2003) Diverged binding specificity of Rim101p, the Candida albicans ortholog of PacC. Eukaryot. Cell 2, 718-728
30. Walther, A., and Wendland, J. (2003) An improved transformation protocol for the human fungal pathogen Candida albicans. Curr. Genet. 42, 339-343 (Pubitemid 36432355)
31. Pullman, M., and Penefsky, H. S. (1963) Preparation and assay of phosphorylating submitochondrial systems: mechanically ruptured mitochondria. In Methods in Enzymology, Vol.6 (Colowick, S. P., and Kaplan, N. O., eds) 6 pp. 277-284, Academic Press, New York
32. Piraud, M., Vianey-Saban, C., Petritis, K., Elfakir, C., Steghens, J. P., Morla, A., and Bouchu, D. (2003) ESI-MS/MS analysis of underivatised amino acids: a new tool for the diagnosis of inherited disorders of amino acid metabolism. Fragmentation study of 79 molecules of biological interest in positive and negative ionisation mode. Rapid Commun. Mass Spectrom. 17, 1297-1311 (Pubitemid 36750014)
33. Van Vlies, N., Wanders, R. J., and Vaz, F. M. (2006) Measurement of carnitine biosynthesis enzyme activities by tandem mass spectrometry: differences between the mouse and the rat. Anal. Biochem. 354, 132-139
34. McNeil, J. B., Flynn, J., Tsao, N., Monschau, N., Stahmann, K., Haynes, R. H., McIntosh, E. M., and Pearlman, R. E. (2000) Glycine metabolism in Candida albicans: characterization of the serine hydroxymethyltransferase (SHM1, SHM2) and threonine aldolase (GLY1) genes. Yeast 16, 167-175 (Pubitemid 30088206)
35. Ballevre, O., Houlier, M. L., Prugnaud, J., Bayle, G., Bercovici, D., Seve, B., and Arnal, M. (1991) Altered partition of threonine metabolism in pigs by protein-free feeding or starvation. Am. J. Physiol. 261, E748-757
36. Davis, A. J., and Austic, R. E. (1994) Dietary threonine imbalance alters threonine dehydrogenase activity in isolated hepatic mitochondria of chicks and rats. J. Nutr. 124, 1667-1677
37. Edgar, A. J. (2005) Mice have a transcribed L-threonine aldolase/GLY1 gene, but the human GLY1 gene is a non-processed pseudogene. BMC Genomics 6, 32
38. Contestabile, R., Paiardini, A., Pascarella, S., di Salvo, M. L., D'Aguanno, S., and Bossa, F. (2001) l-Threonine aldolase, serine hydroxymethyltransferase and fungal alanine racemase. A subgroup of strictly related enzymes specialized for different functions. Eur. J. Biochem. 268, 6508-6525 (Pubitemid 34014759)
39. Rebouche, C. J., and Engel, A. G. (1980) Tissue distribution of carnitine biosynthetic enzymes in man. Biochim. Biophys. Acta 630, 22-29
40. Rebouche, C. J., Lehman, L. J., and Olson, L. (1986) epsilon- N-trimethyllysine availability regulates the rate of carnitine biosynthesis in the growing rat. J. Nutr. 116, 751-759
41. Steiber, A., Kerner, J., and Hoppel, C. L. (2004) Carnitine: a nutritional, biosynthetic, and functional perspective. Mol. Aspects Med. 25, 455-473 (Pubitemid 39195071)