Molecular characterization of carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria in Saccharomyces cerevisiae and identification of a plasma membrane carnitine transporter, Agp2p

EMBO Journal

Volumn 18, Issue 21, 1999, Pages 5843-5852

  Van Roermund, Carlo W T ^a   Hettema, Ewald H ^b   Van Den Berg, Marlene ^b   Tabak, Henk F ^b   Wanders, Ronald J A ^a,b  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b EMMA CHILDREN S HOSPITAL   (Netherlands)

Author keywords

oxidation; Acetyl CoA; Carnitine; Fatty acids; Peroxisomes

Indexed keywords

ACETYL COENZYME A; ACETYLCARNITINE; CARBON DIOXIDE; CARNITINE; CARRIER PROTEIN; FATTY ACID; GLYOXYLIC ACID; WATER;

ARTICLE; CELL MEMBRANE TRANSPORT; CELL TRANSPORT; CYTOPLASM; DNA LIBRARY; FATTY ACID OXIDATION; MITOCHONDRION; NONHUMAN; PEROXISOME; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

ACETYL COENZYME A; AMINO ACID TRANSPORT SYSTEMS; BIOLOGICAL TRANSPORT; CARNITINE; CARNITINE ACYLTRANSFERASES; CARNITINE O-ACETYLTRANSFERASE; CARRIER PROTEINS; CELL MEMBRANE; CLONING, MOLECULAR; FUNGAL PROTEINS; GENES, FUNGAL; MEMBRANE PROTEINS; MICROSCOPY, IMMUNOELECTRON; MITOCHONDRIA; MUTATION; OLEIC ACID; PEROXISOMES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SYMPORTERS;

SACCHAROMYCES CEREVISIAE;

EID: 0033231013     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/18.21.5843     Document Type: Article

References (44)

1. André, B. (1995) An overview of membrane transport proteins in Saccharomyces cerevisiae. Yeast, 11, 1575-1611.
2. Bieber, L.L. (1988) Carnitine. Annu. Rev. Biochem., 57, 261-283.
3. Bremer, J. (1983) Carnitine metabolism and functions. Physiol. Rev., 63, 1420-1480.
4. Dieuaide-noubhani, M., Novikov, D., Baumgart, E., Vanhooren, J.C.T., Fransen, M., Goethals, M., Vandekerkhove, J., Van Veldhoven P.P. and Mannaerts, G.P. (1996) Further characterization of the peroxisomal 3-hydroxyacyl-CoA dehydrogenases from rat liver. Eur. J. Biochem., 240, 660-666.
5. Distel, B. et al. (1996) A unified nomenclature for peroxisome biogenesis factors. J. Cell Biol., 135, 1-3.
6. Elgersma, Y., van Roermund, C.W.T., Wanders, R.J.A. and Tabak, H.F. (1995) Peroxisomal and mitochondrial carnitine acetyltransferases of Saccharomyces cerevisiae are encoded by a single gene. EMBO J., 14, 3472-3479.
7. Elgersma, Y., Vos, A., van den Berg, M., van Roermund, C.W.T., van der Sluijs, P., Distel, B. and Tabak, H.F. (1996) Analysis of the carboxyterminal peroxisomal targetting signal 1 in a homologous context in Saccharomyces cerevisiae. J. Biol. Chem., 271, 26375-26382.
8. Hettema, E.H., Ruigrok, C.C.M., Koerkamp, M.G., Van den Berg, M., Tabak, H.F., Distel, B. and Braakman, I. (1998) The cytosolic DnaJ-like djp1p is involved specifically in peroxisomal protein import. J. Cell Biol., 142, 421-434.
9. Gould, S.J., Keller, G.A., Schneider, M., Howell, S.M., Garrard, L., Goodman, J.M., Distel, B., Tabak, H.F. and Subramani, S. (1990) Peroxisomal protein import is conserved between yeast, insects and mammals. EMBO J., 9, 85-90.
10. Green, N. and Walter, P. (1992) C-terminal sequence can inhibit the insertion of membrane proteins into the endoplasmic reticulum of Saccharomyces cerevisiae. Mol. Cell. Biol., 12, 276-282.
11. Green, N., Hansen, W. and Walter, P. (1989) The use of gene-fusions to determine membrane protein topology in Saccharomyces cerevisiae. J. Cell Sci., 11, 109-113.
12. Indiveri, C., Iacobazzi, V., Giangregorio, N. and Palmieri, F. (1997) The mitochondrial carnitine carrier protein: cDNA cloning, primary structure and comparison with other mitochondrial transporters. Biochem. J., 321, 713-719.
13. Jiang, L.L., Kurosawa, T., Sato, M., Suzuki, Y. and Hashimoto, T. (1997) Physiological role of D-3-hydroxyacyl-CoA dehydratase D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein. J. Biochem., 121, 506-513.
14. Karpichev, I.V. and Small G.M. (1998) Global regulatory functions of Oaf1p and Pip2p (Oaf2p), transcription factors that regulate genes encoding peroxisomal proteins in Saccharomyces cerevisiae. Mol. Cell. Biol., 18, 6560-6570.
15. Kispal, G., Sumegi, B., Dietmeier, K., Bock, I., Gajdos, G., Tomesanyi, T. and Sandor, A. (1993) Cloning and sequencing of a cDNA encoding Saccharomyces cerevisiae carnitine acetyltransferase. Use of cDNA in gene disruption studies. J. Biol. Chem., 268, 1824-1829.
16. Kunau, W.H., Dommes, V. and Schulz, H. (1995) Beta-oxidation of fatty acids in mitochondria, peroxisomes and bacteria: a century of continued progress. Prog. Lipid Res., 34, 267-342.
17. Lawrence, C.W. (1991) Classic mutagenesis techniques. Methods Enzymol., 194, 273-280.
18. Leenders, F., Tesdorpf, J.G., Markus, M., Engel, T., Seedorf, U. and Adamski, J. (1996) Porcine 80-kDa reveals intrinsic 17 beta-hydroxysteroid dehydrogenase, fatty acyl-CoA hydratase/dehydrogenase and sterol transfer activities. J. Biol. Chem., 271, 5438-5442.
19. Ljungdahl, P.O., Gimeno, C.J., Styles, C.A. and Fink, G.R. (1992) SH3: a novel component of the secretory pathway specifically required for localization of amino acid permeases in yeast. Cell, 71, 463-478.
20. Molstad, P., Bomer, T. and Hovig, T. (1978) Carnitine-induced uptake of L-carnitine into cells from an established cell line from human heart. Biochim. Biophys. Acta, 512, 557-565.
21. Nelissen, B., De Wachter, R. and Goffeau, A. (1997) Classification of all putative permeases and other membrane plurispanners of the major facilitator superfamily encoded by the complete genome of S.cerevisiae. FEMS Microb. Rev., 21, 113-134.
22. Osmundsen, H., Bremer, J. and Pedersen, J.I. (1991) Metabolic aspects of peroxisomal beta-oxidation. Biochim. Biophys. Acta, 1085, 141-158.
23. Palmieri, L., Lasorsa, F.M., De Palma, A., Palmieri, F., Runswick, M.J. and Walker, J.E. (1997) Identification of yeast ACR1 gene product as a succinate-fumarate transporter essential for growth on ethanol or acetate. FEBS Lett., 417, 114-118.
24. Prasad, P.D., Huang, W., Ramamoorthy, S., Carter, A., Leibach, F.H. and Ganapathy, V. (1996) Sodium-dependent carnitine transport in human placental choriocarcinoma cells. Biochim. Biophys. Acta, 1284, 109-117.
25. Rebouche, C.J. (1977) Carnitine movement across muscle plasma membrane. Studies in isolated rat muscle. Biochim. Biophys. Acta, 471, 145-155.
26. Reddy, J.K. and Mannaerts, G.P. (1994) Peroxisomal lipid metabolism. Annu. Rev. Nutr., 14, 343-370.
27. Schulz, H. (1991) Beta-oxidation of fatty acids. Biochim. Biophys. Acta, 1081, 109-120.
28. Seedorf, U., Brysch, P., Engel, T., Schrage, K. and Assmann, G. (1994) Sterol carrier protein x is peroxisomal 3-oxoacyl-CoA thiolase with intrinsic sterol carrier and lipid transfer activity. J. Biol. Chem., 269, 21277-21283.
29. Smith, P.K. (1985) Measurements of protein using bicinchonic acid. Anal. Biochem., 150, 76-85.
30. Stieger, B., O'Neill, B. and Knahenbuhl, S. (1995) Characterization of L-carnitine transport by rat kidney brush-border-membrane vesicles. Biochem. J., 309, 643-647.
31. Tamai I., Ohashi, R., Nezu, J., Oku, A., Shimane, M., Sai, Y. and Tsuji, A. (1998) Molecular and functional identification of sodium-ion-dependent, high affinity human carnitine transporter OCTN2. J. Biol. Chem., 271, 20378-20382.
32. Tein, I., Bukovac, S.W. and Xie, Z.-W. (1996) Characterization of the human plasmalemmal carnitine transporter in cultured skin fibroblasts. Arch. Biochem. Biophys, 329, 145-155.
33. Van der Leij, I., Van der Berg, M., Boot, R., Franse, M.M., Distel, B. and Tabak, H.F. (1992) Isolation of peroxisome assembly mutants from Saccharomyces cerevisiae with different morphologies using a novel positive selection procedure. J. Cell Biol., 119, 153-162.
34. van Roermund, C.W.T., Elgersma, Y., Singh, N., Wanders, R.J.A. and Tabak, H.F. (1995) The membrane of peroxisomes in Saccharomyces cerevisiae is impermeable to NAD(H) and acetyl-CoA under in vivo conditions. EMBO J., 14, 3480-3486.
35. van Roermund, C.W.T., Hettema, E.H., Kal, A.J., van den Berg, M., Tabak, H.F. and Wanders, R.J.A. (1998) Peroxisomal β-oxidation of polyunsaturated fatty acids in Saccharomyces cerevisiae: evidence for an isocitrate/2-oxoglutarate NADP redox shuttle. EMBO J., 17, 677-687.
36. Vary, T.C. and Neely, J.R. (1982) Characterization of carnitine transport in isolated perfused adult rat hearts. Am. J. Physiol., 242, H585-H592.
37. Verhoeven, N., Schor, D., Roe, C.R., Wanders, R.J.A. and Jacobs, C. (1998) Pristanic acid β-oxidation in peroxisomal disorders: studies in cultured human fibroblasts. Biochim. Biophys. Acta, 1391, 351-356.
38. Verleur, N., Hettema, E.H., van Roermund, C.W.T., Tabak, H.F. and Wanders, R.J.A. (1997) Transport of activated fatty acids by peroxisomal ATP-binding-cassette transporter Pxa2p in semi-intact yeast cell system. Eur. J. Biochem., 249, 657-661.
39. Voom-Brouwer, T., Van der Leij, I., Hemrika, W., Distel, B. and Tabak, H.F. (1993) Sequence of the PAS8 gene, the product of which is essential for the biogenesis of peroxisomes in Saccaromyces cerevisiae. Biochim. Biophys. Acta, 1216, 325-328.
40. Vreken, P., van Lint, A.E.M., Bootsma, A.H., Overmars, H., Wanders, R.J.A. and van Gennip, A.H. (1999) Quantitative plasma acylcarnitine analysis using electrospray tandem mass spectrometry for the diagnosis of organic acidaemias and fatty oxidation defects. J. Inher. Metab. Dis., 22, 302-306.
41. Wach, A. (1996) Yeast functional analysis reports. Yeast, 12, 259-265.
42. Wanders, R.J.A., IJlst, L., Poggi, F., Bonnefont, J.P., Munnich, A., Brivet, M., Rabier, D. and Saudubray, J.M. (1992) Human trifunctional protein deficiency: a new disorder of mitochondrial fatty acid beta-oxidation. Biochem. Biophys. Res. Commun., 188, 1139-1145.
43. Wanders, R.J.A., Schutgens, R.B.H. and Barth, P.G. (1995) Peroxisomal disorders: a review. J. Neuropathol. Exp. Neurol., 54, 726-739.
44. Wanders, R.J.A., Denis, S., Wouters, F., Wirtz, K.W.A. and Seedorf, U. (1997) Sterol carrier protein x (SCPx) is a peroxisomal branched-chain beta keto-thiolase specifically reacting with 3-oxo-pristanoyl-CoA: a new unique role for SCPx in branched-chain fatty acid metabolism in peroxisomes. Biochem. Biophys. Res. Commun., 236, 565-569.