MTS dye based colorimetric CTLL-2 cell proliferation assay for product release and stability monitoring of Interleukin-15: Assay qualification, standardization and statistical analysis

Journal of Immunological Methods

Volumn 348, Issue 1-2, 2009, Pages 83-94

  Soman, Gopalan ^a   Yang, Xiaoyi ^a   Jiang, Hengguang ^a   Giardina, Steve ^a   Vyas, Vinay ^a   Mitra, George ^a   Yovandich, Jason ^c   Creekmore, Stephen P ^c   Waldmann, Thomas A ^d   Quiñones, Octavio ^b   Alvord, W Gregory ^b  

^a SAIC FREDERICK INC   (United States)

^b Data Management Services Inc   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

^d NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Assay qualification; Cell proliferation assay; IL 15; R statistical language and environment; Statistical analysis

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; INTERLEUKIN 15; NEUTRALIZING ANTIBODY; PHENAZINE;

ACCURACY; ARTICLE; CELL PROLIFERATION; COLORIMETRY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOKINE RELEASE; CYTOTOXIC T LYMPHOCYTE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN STABILITY; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; STANDARDIZATION; STATISTICAL ANALYSIS;

ANTIBODIES; BIOLOGICAL ASSAY; CELL LINE, TUMOR; CELL PROLIFERATION; COLORIMETRY; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; INTERLEUKIN-15; INTERLEUKIN-2; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TETRAZOLIUM SALTS; THIAZOLES;

EID: 68749110804     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.07.010     Document Type: Article

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