Association of virulence plasmid and antibiotic resistance determinants with chromosomal multilocus genotypes in Mexican salmonella enterica serovar typhimurium strains

BMC Microbiology

Volumn 9, Issue , 2009, Pages

  Wiesner, Magdalena ^a   Zaidi, Mussaret B ^b   Calva, Edmundo ^a   Fernández Mora, Marcos ^a   Calva, Juan J ^c   Silva, Claudia ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b Hospital General O'Horan   (Mexico)

^c INSTITUTO NACIONAL DE CIENCIAS MÉDICAS Y NUTRICIÓN SALVADOR ZUBIRÁN   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

BETA LACTAMASE; CMY 2 ENZYME; UNCLASSIFIED DRUG; BACTERIAL DNA;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATION; CONTROLLED STUDY; GENE LOCUS; GENE MAPPING; GENE SEQUENCE; GENETIC VARIABILITY; GENOMIC ISLAND; MULTILOCUS SEQUENCE TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PULSED FIELD GEL ELECTROPHORESIS; SALMONELLA ENTERICA; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GENOME; BACTERIUM IDENTIFICATION; CLASSIFICATION; DNA SEQUENCE; GENETIC MARKER; GENETICS; GENOTYPE; INTEGRON; MEXICO; MICROBIOLOGICAL EXAMINATION; PATHOGENICITY; PLASMID; POPULATION GENETICS; SALMONELLA TYPHIMURIUM; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM;

BACTERIAL TYPING TECHNIQUES; CHROMOSOMES, BACTERIAL; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; GENETIC MARKERS; GENETIC VARIATION; GENETICS, POPULATION; GENOME, BACTERIAL; GENOMIC ISLANDS; GENOTYPE; INTEGRONS; MEXICO; MICROBIAL SENSITIVITY TESTS; PLASMIDS; SALMONELLA TYPHIMURIUM; SEQUENCE ANALYSIS, DNA; VIRULENCE;

EID: 68549122606     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-9-131     Document Type: Article

References (80)

1. The microbial pan-genome. D Medini C Donati H Tettelin V Masignani R Rappuoli, Curr Opin Genet Dev 2005 15 589 594 10.1016/j.gde.2005.09.006 16185861 (Pubitemid 41532330)
2. Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae : implications for the microbial "pan-genome". H Tettelin V Masignani MJ Cieslewicz C Donati D Medini NL Ward SV Angiuoli J Crabtree AL Jones AS Durkin, Proc Natl Acad Sci USA 2005 102 13950 13955 16172379 10.1073/pnas.0506758102 (Pubitemid 41377684)
3. The genome of Rhizobium leguminosarum has recognizable core and accessory components. JP Young LC Crossman AW Johnston NR Thomson ZF Ghazoui KH Hull M Wexler AR Curson JD Todd PS Poole, Genome Biol 2006 7 R34 16640791 10.1186/gb-2006-7-4-r34 (Pubitemid 43881251)
4. Bacteria are different: observations, interpretations, speculations, and opinions about the mechanisms of adaptive evolution in prokaryotes. BR Levin CT Bergstrom, Proc Natl Acad Sci USA 2000 97 6981 6985 10860960 10.1073/pnas.97.13.6981 (Pubitemid 30431405)
5. Small change: keeping pace with microevolution. EJ Feil, Nat Rev Microbiol 2004 2 483 495 10.1038/nrmicro904 15152204 (Pubitemid 39200015)
6. How clonal are bacteria? J Maynard-Smith NH Smith M O'Rourke BG Spratt, Proc Natl Acad Sci USA 1993 90 4384 4388 8506277 10.1073/pnas.90.10.4384
7. Evolutionary genetics of Salmonella enterica. RK Selander J Li K Nelson, Escherichia coli and Salmonella: Celular and Molecular Biology Washington, DC: American Society of Microbiology, Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE, 1996 2691 2707
8. Bacterial population genetics, evolution and epidemiology. BG Spratt MC Maiden, Philos Trans R Soc Lond B Biol Sci 1999 354 701 710 10365396 10.1098/rstb.1999.0423
9. Supplement 2002 (no. 46) to the Kauffmann-White scheme. MY Popoff J Bockemuhl LL Gheesling, Res Microbiol 2004 155 568 570 10.1016/j.resmic.2004.04. 005 15313257 (Pubitemid 39094208)
10. Multilocus sequence typing supports the hypothesis that cow- and human-associated Salmonella isolates represent distinct and overlapping populations. SD Alcaine Y Soyer LD Warnick WL Su S Sukhnanand J Richards ED Fortes P McDonough TP Root NB Dumas, Appl Environ Microbiol 2006 72 7575 7585 17028236 10.1128/AEM.01174-06 (Pubitemid 44927659)
11. Ceftiofur-resistant Salmonella strains isolated from dairy farms represent multiple widely distributed subtypes that evolved by independent horizontal gene transfer. SD Alcaine SS Sukhnanand LD Warnick WL Su P McGann P McDonough M Wiedmann, Antimicrob Agents Chemother 2005 49 4061 4067 16189081 10.1128/AAC.49.10.4061-4067.2005 (Pubitemid 41400947)
12. DNA sequence-based subtyping and evolutionary analysis of selected Salmonella enterica serotypes. S Sukhnanand S Alcaine LD Warnick WL Su J Hof MP Craver P McDonough KJ Boor M Wiedmann, J Clin Microbiol 2005 43 3688 3698 16081897 10.1128/JCM.43.8.3688-3698.2005 (Pubitemid 41129647)
13. Comparison of multilocus sequence typing, pulsed-field gel electrophoresis, and antimicrobial susceptibility typing for characterization of Salmonella enterica serotype Newport isolates. H Harbottle DG White PF McDermott RD Walker S Zhao, J Clin Microbiol 2006 44 2449 2457 16825363 10.1128/JCM.00019-06 (Pubitemid 44092410)
14. Evolution of host adaptation in Salmonella enterica. AJ Baumler RM Tsolis TA Ficht LG Adams, Infect Immun 1998 66 4579 4587 9746553
15. Host adaptation and the emergence of infectious disease: the Salmonella paradigm. RA Kingsley AJ Baumler, Mol Microbiol 2000 36 1006 1014 10.1046/j.1365-2958.2000.01907.x 10844686 (Pubitemid 30330672)
16. Salmonella enterica serotype Typhimurium and its host-adapted variants. W Rabsch HL Andrews RA Kingsley R Prager H Tschape LG Adams AJ Baumler, Infect Immun 2002 70 2249 2255 11953356 10.1128/IAI.70.5.2249-2255.2002 (Pubitemid 34408361)
17. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. MC Maiden JA Bygraves E Feil G Morelli JE Russell R Urwin Q Zhang J Zhou K Zurth DA Caugant, Proc Natl Acad Sci USA 1998 95 3140 3145 9501229 10.1073/pnas.95.6.3140 (Pubitemid 28135868)
18. Characterization and localization of drug resistance determinants in multidrug-resistant, integron-carrying Salmonella enterica serotype Typhimurium strains. B Guerra E Junker A Miko R Helmuth MC Mendoza, Microb Drug Resist 2004 10 83 91 10.1089/1076629041310136 15256022 (Pubitemid 38879007)
19. Evolution of the virulence plasmids of non-typhoid Salmonella and its association with antimicrobial resistance. C Chu CH Chiu, Microbes Infect 2006 8 1931 1936 10.1016/j.micinf.2005.12.026 16713725 (Pubitemid 44128867)
20. Molecular analysis of spv virulence genes of the Salmonella virulence plasmids. PA Gulig H Danbara DG Guiney AJ Lax F Norel M Rhen, Mol Microbiol 1993 7 825 830 10.1111/j.1365-2958.1993.tb01172.x 8483415
21. Differences in the carriage and the ability to utilize the serotype associated virulence plasmid in strains of Salmonella enterica serotype Typhimurium investigated by use of a self-transferable virulence plasmid, pOG669. JE Olsen DJ Brown LE Thomsen DJ Platt MS Chadfield, Microb Pathog 2004 36 337 347 10.1016/j.micpath.2004.02.005 15120160 (Pubitemid 38543061)
22. Strain differences in expression of virulence by the 90 kilobase pair virulence plasmid of Salmonella serovar Typhimurium. JT Ou LS Baron, Microb Pathog 1991 10 247 251 10.1016/0882-4010(91)90058-I 1654496
23. Distribution and function of plasmids in Salmonella enterica. I Rychlik D Gregorova H Hradecka, Vet Microbiol 2006 112 1 10 10.1016/j.vetmic.2005.10.030 16303262 (Pubitemid 41814709)
24. Lack of evidence of an association between the carriage of virulence plasmid and the bacteremia of Salmonella typhimurium in humans. CH Chiu C Chu JT Ou, Microbiol Immunol 2000 44 741 748 11092237
25. Prevalence of the virulence plasmids of nontyphoid Salmonella in the serovars isolated from humans and their association with bacteremia. CH Chiu TY Lin JT Ou, Microbiol Immunol 1999 43 899 903 10553683
26. Extra-intestinal Salmonella infections: the significance of spv genes. J Fierer, Clin Infect Dis 2001 32 519 520 10.1086/318505 11170966 (Pubitemid 32173645)
27. Diverse virulence traits underlying different clinical outcomes of Salmonella infection. J Fierer DG Guiney, J Clin Invest 2001 107 775 780 11285291 10.1172/JCI12561
28. Characterization of a self-transferable plasmid from Salmonella enterica serotype typhimurium clinical isolates carrying two integron-borne gene cassettes together with virulence and drug resistance genes. B Guerra S Soto R Helmuth MC Mendoza, Antimicrob Agents Chemother 2002 46 2977 2981 12183256 10.1128/AAC.46.9.2977-2981.2002 (Pubitemid 34898681)
29. Rapid and widespread dissemination of multidrug-resistant blaCMY-2 Salmonella Typhimurium in Mexico. MB Zaidi V Leon C Canche C Perez S Zhao SK Hubert J Abbott K Blickenstaff PF McDermott, J Antimicrob Chemother 2007 60 398 401 10.1093/jac/dkm168 17526503 (Pubitemid 47243892)
30. Characterization of plasmids carrying CMY-2 from expanded-spectrum cephalosporin-resistant Salmonella strains isolated in the United States between 1996 and 1998. A Carattoli F Tosini WP Giles ME Rupp SH Hinrichs FJ Angulo TJ Barrett PD Fey, Antimicrob Agents Chemother 2002 46 1269 1272 11959555 10.1128/AAC.46.5.1269-1272.2002 (Pubitemid 34415301)
31. Animal and human multidrug-resistant, cephalosporin-resistant salmonella isolates expressing a plasmid-mediated CMY-2 AmpC beta-lactamase. PL Winokur A Brueggemann DL DeSalvo L Hoffmann MD Apley EK Uhlenhopp MA Pfaller GV Doern, Antimicrob Agents Chemother 2000 44 2777 2783 10991860 10.1128/AAC.44.10.2777- 2783.2000 (Pubitemid 30737699)
32. Class 1 integrons, gene cassettes, mobility, and epidemiology. AC Fluit FJ Schmitz, Eur J Clin Microbiol Infect Dis 1999 18 761 770 10.1007/ s100960050398 10614949
33. Evidence of extensive interspecies transfer of integron-mediated antimicrobial resistance genes among multidrug-resistant Enterobacteriaceae in a clinical setting. MA Leverstein-van Hall AT Box HE Blok A Paauw AC Fluit J Verhoef, J Infect Dis 2002 186 49 56 10.1086/341078 12089661 (Pubitemid 34666913)
34. Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin. MA Leverstein-van Hall MB HE TD AR A Paauw AC Fluit J Verhoef, J Infect Dis 2003 187 251 259 10.1086/345880 12552449 (Pubitemid 36132638)
35. Resistance integrons and super-integrons. AC Fluit FJ Schmitz, Clin Microbiol Infect 2004 10 272 288 10.1111/j.1198-743X.2004.00858.x 15059115 (Pubitemid 38519353)
36. Towards more virulent and antibiotic-resistant Salmonella? AC Fluit, FEMS Immunol Med Microbiol 2005 43 1 11 10.1016/j.femsim.2004.10.007 15607630 (Pubitemid 40029421)
37. Characterization of antimicrobial resistance and class 1 and 2 integrons in Salmonella enterica isolates from different sources in Portugal. P Antunes J Machado L Peixe, J Antimicrob Chemother 2006 58 297 304 10.1093/jac/dkl242 16782741 (Pubitemid 44294938)
38. Characterization of class I integrons in clinical strains of Salmonella enterica subsp. enterica serovars Typhimurium and Enteritidis from Norwegian hospitals. BA Lindstedt E Heir I Nygard G Kapperud, J Med Microbiol 2003 52 141 149 10.1099/jmm.0.04958-0 12543920 (Pubitemid 36205262)
39. Class 1 integrons and resistance gene cassettes among multidrug resistant Salmonella serovars isolated from slaughter animals and foods of animal origin in Ethiopia. B Molla A Miko K Pries G Hildebrandt J Kleer A Schroeter R Helmuth, Acta Trop 2007 103 142 149 10.1016/j.actatropica.2007.05.018 17658448 (Pubitemid 47268232)
40. Analysis of integrons in clinical isolates of Escherichia coli in China during the last six years. J Su L Shi L Yang Z Xiao X Li S Yamasaki, FEMS Microbiol Lett 2006 254 75 80 10.1111/j.1574-6968.2005.00025.x 16451182
41. Characterization of multidrug resistant Salmonella recovered from diseased animals. S Zhao PF McDermott DG White S Qaiyumi SL Friedman JW Abbott A Glenn SL Ayers KW Post WH Fales, Vet Microbiol 2007 123 122 132 10.1016/j.vetmic.2007.03.001 17400409 (Pubitemid 46898719)
42. The Salmonella genomic island 1 is an integrative mobilizable element. B Doublet D Boyd MR Mulvey A Cloeckaert, Mol Microbiol 2005 55 1911 1924 10.1111/j.1365-2958.2005.04520.x 15752209 (Pubitemid 40445222)
43. Complete nucleotide sequence of a 43-kilobase genomic island associated with the multidrug resistance region of Salmonella enterica serovar Typhimurium DT104 and its identification in phage type DT120 and serovar Agona. D Boyd GA Peters A Cloeckaert KS Boumedine E Chaslus-Dancla H Imberechts MR Mulvey, J Bacteriol 2001 183 5725 5732 11544236 10.1128/JB.183.19.5725-5732.2001 (Pubitemid 32898620)
44. The genetics of Salmonella genomic island 1. MR Mulvey DA Boyd AB Olson B Doublet A Cloeckaert, Microbes Infect 2006 8 1915 1922 10.1016/j.micinf.2005. 12.028 16713724 (Pubitemid 44144557)
45. Salmonella MLST database. http://mlst.ucc.ie/mlst/dbs/Senterica
46. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. M McClelland KE Sanderson J Spieth SW Clifton P Latreille L Courtney S Porwollik J Ali M Dante F Du, Nature 2001 413 852 856 10.1038/35101614 11677609 (Pubitemid 33026853)
47. Association of adhesive, invasive, and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plasmids. GW Jones DK Rabert DM Svinarich HJ Whitfield, Infect Immun 1982 38 476 486 6128304
48. Plasmid-mediated florfenicol and ceftriaxone resistance encoded by the floR and bla(CMY-2) genes in Salmonella enterica serovars Typhimurium and Newport isolated in the United States. B Doublet A Carattoli JM Whichard DG White S Baucheron E Chaslus-Dancla A Cloeckaert, FEMS Microbiol Lett 2004 233 301 305 10.1111/j.1574-6968.2004.tb09496.x 15063500
49. Dissemination of sul3-containing elements linked to class 1 integrons with an unusual 3' conserved sequence region among Salmonella isolates. P Antunes J Machado L Peixe, Antimicrob Agents Chemother 2007 51 1545 1548 17283193 10.1128/AAC.01275-06 (Pubitemid 46586849)
50. Characterization of class 1 integrons with unusual 3' conserved region from Salmonella enterica isolates. R Chuanchuen C Koowatananukul S Khemtong, Southeast Asian J Trop Med Public Health 2008 39 419 424 18564680
51. Integron-bearing methicillin-resistant coagulase-negative staphylococci in South China, 20012004. Z Xu L Shi MJ Alam L Li S Yamasaki, FEMS Microbiol Lett 2008 278 223 230 10.1111/j.1574-6968.2007.00994.x 18096018 (Pubitemid 350264580)
52. Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan: description of an unusual class 2 integron. AM Ahmed H Nakano T Shimamoto, J Antimicrob Chemother 2005 55 371 374 10.1093/jac/dkh534 15705645 (Pubitemid 40430691)
53. Salmonella Typhi, the causative agent of typhoid fever, is approximately 50,000 years old. C Kidgell U Reichard J Wain B Linz M Torpdahl G Dougan M Achtman, Infect Genet Evol 2002 2 39 45 10.1016/S1567-1348(02)00089-8 12797999
54. Prophage sequences defining hot spots of genome variation in Salmonella enterica serovar Typhimurium can be used to discriminate between field isolates. FJ Cooke J Wain M Fookes A Ivens N Thomson DJ Brown EJ Threlfall G Gunn G Foster G Dougan, J Clin Microbiol 2007 45 2590 2598 17522270 10.1128/JCM.00729-07 (Pubitemid 47295492)
55. Evolutionary genomics of Salmonella: gene acquisitions revealed by microarray analysis. S Porwollik RM Wong M McClelland, Proc Natl Acad Sci USA 2002 99 8956 8961 12072558 10.1073/pnas.122153699 (Pubitemid 34693665)
56. Genetic flux over time in the Salmonella lineage. GS Vernikos NR Thomson J Parkhill, Genome Biol 2007 8 R100 17547764 10.1186/gb-2007-8-6-r100 (Pubitemid 351903183)
57. Integrated food chain surveillance system for Salmonella spp. in Mexico. MB Zaidi JJ Calva MT Estrada-Garcia V Leon G Vazquez G Figueroa E Lopez J Contreras J Abbott S Zhao, Emerg Infect Dis 2008 14 429 435 18325258 10.3201/eid1403.071057
58. Non-typhoidal salmonellosis: emerging problems. W Rabsch H Tschape AJ Baumler, Microbes Infect 2001 3 237 247 10.1016/S1286-4579(01)01375-2 11358718 (Pubitemid 32453152)
59. The clonal spread of multidrug-resistant non-typhi Salmonella serotypes. P Butaye GB Michael S Schwarz TJ Barrett A Brisabois DG White, Microbes Infect 2006 8 1891 1897 10.1016/j.micinf.2005.12.020 16714135 (Pubitemid 44144555)
60. Use of antibiotic susceptibility patterns and pulsed-field gel electrophoresis to compare historic and contemporary isolates of multi-drug-resistant Salmonella enterica subsp. enterica serovar Newport. AC Berge JM Adaska WM Sischo, Appl Environ Microbiol 2004 70 318 323 14711658 10.1128/AEM.70.1.318-323.2004 (Pubitemid 38090220)
61. Changes in the clonal nature and antibiotic resistance profiles of methicillin-resistant Staphylococcus aureus isolates associated with spread of the EMRSA-15 clone in a tertiary care Portuguese hospital. ML Amorim NA Faria DC Oliveira C Vasconcelos JC Cabeda AC Mendes E Calado AP Castro MH Ramos JM Amorim H de Lencastre, J Clin Microbiol 2007 45 2881 2888 17626175 10.1128/JCM.00603-07 (Pubitemid 47417330)
62. Tracking methicillin-resistant Staphylococcus aureus clones during a 5-year period (1998 to 2002) in a Spanish hospital. E Perez-Roth F Lorenzo-Diaz N Batista A Moreno S Mendez-Alvarez, J Clin Microbiol 2004 42 4649 4656 15472324 10.1128/JCM.42.10.4649-4656.2004 (Pubitemid 39362386)
63. Emergence of multidrug-resistant Salmonella enterica serotype Newport infections resistant to expanded-spectrum cephalosporins in the United States. A Gupta J Fontana C Crowe B Bolstorff A Stout S Van Duyne MP Hoekstra JM Whichard TJ Barrett FJ Angulo, J Infect Dis 2003 188 1707 1716 10.1086/379668 14639542 (Pubitemid 38010682)
64. Characterization of Salmonella enterica serotype newport isolated from humans and food animals. S Zhao S Qaiyumi S Friedman R Singh SL Foley DG White PF McDermott T Donkar C Bolin S Munro, J Clin Microbiol 2003 41 5366 5371 14662912 10.1128/JCM.41.12.5366-5371.2003 (Pubitemid 37543218)
65. Genes and mutations conferring antimicrobial resistance in Salmonella : an update. GB Michael P Butaye A Cloeckaert S Schwarz, Microbes Infect 2006 8 1898 1914 10.1016/j.micinf.2005.12.019 16716631 (Pubitemid 44144554)
66. Human Salmonella clinical isolates distinct from those of animal origin. DM Heithoff WR Shimp PW Lau G Badie EY Enioutina RA Daynes BA Byrne JK House MJ Mahan, Appl Environ Microbiol 2008 74 1757 1766 18245251 10.1128/AEM.02740-07
67. Integrons and gene cassettes in the Enterobacteriaceae. PA White CJ McIver WD Rawlinson, Antimicrob Agents Chemother 2001 45 2658 2661 11502548 10.1128/AAC.45.9.2658-2661.2001 (Pubitemid 32801929)
68. Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination. RM Hall CM Collis, Mol Microbiol 1995 15 593 600 10.1111/j.1365-2958.1995.tb02368.x 7783631
69. Transposon Tn21, flagship of the floating genome. CA Liebert RM Hall AO Summers, Microbiol Mol Biol Rev 1999 63 507 522 10477306
70. Mobile gene cassettes: a fundamental resource for bacterial evolution. CA Michael MR Gillings AJ Holmes L Hughes NR Andrew MP Holley HW Stokes, Am Nat 2004 164 1 12 10.1086/421733 15266366 (Pubitemid 38991827)
71. Detection of multidrug-resistant Salmonella enterica serovar typhimurium phage types DT102, DT104, and U302 by multiplex PCR. CH Chiu LH Su CH Chu MH Wang CM Yeh FX Weill C Chu, J Clin Microbiol 2006 44 2354 2358 16825349 10.1128/JCM.00171-06 (Pubitemid 44092398)
72. Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar Typhimurium DT104. LK Ng MR Mulvey I Martin GA Peters W Johnson, Antimicrob Agents Chemother 1999 43 3018 3021 10582902
73. Nontyphoidal Salmonella from human clinical cases, asymptomatic children, and raw retail meats in Yucatan, Mexico. MB Zaidi PF McDermott P Fedorka-Cray V Leon C Canche SK Hubert J Abbott M Leon S Zhao M Headrick L Tollefson, Clin Infect Dis 2006 42 21 28 10.1086/498508 16323087 (Pubitemid 41832075)
74. Clinical-and-Laboratory-Standards-Institute, Performance standards for antimicrobial disk susceptibility tests Wayne, PA: Clinical and Laboratory Standards Institute 2006
75. NCCLS, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Document M7-A6. Approved standard-Sixth edition Wayne, PA: NCCLS 2002
76. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. TA Hall, Nucl Acids Symp Ser 1999 41 95 98
77. eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. EJ Feil BC Li DM Aanensen WP Hanage BG Spratt, J Bacteriol 2004 186 1518 1530 14973027 10.1128/JB.186.5.1518-1530.2004 (Pubitemid 38248742)
78. CDC, Standardized molecular subtyping of foodborne bacterial pathogens by pulsed-field gel electrophoresis: a manual Atlanta, GA: National Center for Infectious Diseases 1996 (updated 2000).
79. J Sambrook DW Russell, Molecular cloning. A laboratory manual New York: Cold Spring Harbor Laboratory Press Third 2001
80. National Center for Biotechnology Information. http://www.ncbi.nlm.nih. gov