A novel Cre recombinase imaging system for tracking lymphotropic virus infection in vivo

PLoS ONE

Volumn 4, Issue 8, 2009, Pages

  Dutia, Bernadette M ^a   Reid, Stuart J ^a   Drummond, Derek D ^b   Ligertwood, Yvonne ^a   Bennet, Ian ^a   Rietberg, Willard ^a   Silvia, Ondine ^a   Jarvis, Michael A ^b   Nash, Anthony A ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; INTEGRASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; B LYMPHOCYTE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; GENE EXPRESSION; HERPES VIRUS; IMAGING SYSTEM; IN VIVO STUDY; MACROPHAGE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; VIRUS GENE; VIRUS INFECTION; VIRUS REPLICATION; ANIMAL; BAGG ALBINO MOUSE; C57BL MOUSE; CELL LINE; FLOW CYTOMETRY; GAMMAHERPESVIRINAE; GENETIC RECOMBINATION; GENETICS; HAMSTER; HERPES VIRUS INFECTION; IMMUNOHISTOCHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; VIROLOGY;

MURINAE;

ANIMALS; B-LYMPHOCYTES; BASE SEQUENCE; BLOTTING, SOUTHERN; CELL LINE; CRICETINAE; FLOW CYTOMETRY; GAMMAHERPESVIRINAE; GREEN FLUORESCENT PROTEINS; HERPESVIRIDAE INFECTIONS; IMMUNOHISTOCHEMISTRY; INTEGRASES; MACROPHAGES; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC;

EID: 68349157470     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0006492     Document Type: Article

References (37)

1. Dienglewicz RL, Parcells MS (1999) Establishment of a lymphoblastoid cell line using a mutant MDV containing a green fluorescent protein expression cassette. Acta Virol 43: 106-112.
2. Lachmann RH, Efstathiou S (1997) Utilization of the herpes simplex virus type 1 latency-associated regulatory region to drive stable reporter gene expression in the nervous system. J Virol 71: 3197-3207.
3. Shimeld C, Efstathiou S, Hill T (2001) Tracking the spread of a lacZ-tagged herpes simplex virus type 1 between the eye and the nervous system of the mouse: comparison of primary and recurrent infection. J Virol 75: 5252-5262.
4. Marshall KR, Lachmann RH, Efstathiou S, Rinaldi A, Preston CM (2000) Long-term transgene expression in mice infected with a herpes simplex virus type 1 mutant severely impaired for immediate-early gene expression. J Virol 74: 956-964.
5. Boldogkoi Z, Erdelyi F, Sik A, Freund TF, Fodor I (1999) Construction of a recombinant herpesvirus expressing the jellyfish green fluorescent protein. Luminescence 14: 69-74.
6. Parcells MS, Dienglewicz RL, Anderson AS, Morgan RW (1999) Recombinant Marek's disease virus (MDV)-derived lymphoblastoid cell lines: regulation of a marker gene within the context of the MDV genome. J Virol 73: 1362-1373.
7. Lachmann RH, Brown C, Efstathiou S (1996) A murine RNA polymerase I promoter inserted into the herpes simplex virus type 1 genome is functional during lytic, but not latent, infection. J Gen Virol 77 (Pt 10): 2575-2582.
8. Ecob-Prince MS, Hassan K, Denheen MT, Preston CM (1995) Expression of beta-galactosidase in neurons of dorsal root ganglia which are latently infected with herpes simplex virus type 1. J Gen Virol 76 (Pt 6): 1527-1532.
9. Lokensgard JR, Bloom DC, Dobson AT, Feldman LT (1994) Long-term promoter activity during herpes simplex virus latency. J Virol 68: 7148-7158.
10. Smith C, Lachmann RH, Efstathiou S (2000) Expression from the herpes simplex virus type 1 latency-associated promoter in the murine central nervous system. J Gen Virol 81: 649-662.
11. Scarpini CG, May J, Lachmann RH, Preston CM, Dunnett SB, et al. (2001) Latency associated promoter transgene expression in the central nervous system after stereotaxic delivery of replication-defective HSV-1-based vectors. Gene Ther 8: 1057-1071.
12. Rajewsky K, Gu H, Kuhn R, Betz UA, Muller W, et al. (1996) Conditional gene targeting. J Clin Invest 98: 600-603.
13. Kuhn R, Torres RM (2002) Cre/loxP recombination system and gene targeting. Methods Mol Biol 180: 175-204.
14. Metzger D, Chambon P (2001) Site- and time-specific gene targeting in the mouse. Methods 24: 71-80.
15. Efstathiou S, Ho YM, Hall S, Styles CJ, Scott SD, et al. (1990) Murine herpesvirus 68 is genetically related to the gammaherpesviruses Epstein-Barr virus and herpesvirus saimiri. Journal of General Virology 71: 1365-1372.
16. Nash AA, Dutia BM, Stewart JP, Davison AJ (2001) Natural history of murine gamma-herpesvirus infection. Philos Trans R Soc Lond B Biol Sci 356: 569-579.
17. Marques S, Efstathiou S, Smith KG, Haury M, Simas JP (2003) Selective gene expression of latent murine gammaherpesvirus 68 in B lymphocytes. J Virol 77: 7308-7318.
18. Flano E, Kim IJ, Woodland DL, Blackman MA (2002) Gamma-herpesvirus latency is preferentially maintained in splenic germinal center and memory B cells. J Exp Med 196: 1363-1372.
19. Flano E, Kim IJ, Moore J, Woodland DL, Blackman MA (2003) Differential gamma-herpesvirus distribution in distinct anatomical locations and cell subsets during persistent infection in mice. J Immunol 170: 3828-3834.
20. Gilchrist DS, Ure J, Hook L, Medvinsky A (2003) Labeling of hematopoietic stem and progenitor cells in novel activatable EGFP reporter mice. Genesis 36: 168-176.
21. Sunil-Chandra NP, Efstathiou S, Arno J, Nash AA (1992) Virological and pathological features of mice infected with murine gamma-herpesvirus 68. Journal of General Virology 73: 2347-2356.
22. Adler H, Messerle M, Wagner M, Koszinowski UH (2000) Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome. Journal of Virology 74: 6964-6974.
23. Smith GA, Enquist LW (2000) A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis. Proc Natl Acad Sci U S A 97: 4873-4878.
24. Rue CA, Jarvis MA, Knoche AJ, Meyers HL, DeFilippis VR, et al. (2004) A cyclooxygenase-2 homologue encoded by rhesus cytomegalovirus is a determinant for endothelial cell tropism. J Virol 78: 12529-12536.
25. Wagner M, Ruzsics Z, Koszinowski UH (2002) Herpesvirus genetics has come of age. Trends Microbiol 10: 318-324.
26. Stevenson PG, May JS, Smith XG, Marques S, Adler H, et al. (2002) K3-mediated evasion of CD8(+) T cells aids amplification of a latent gammaherpesvirus. Nat Immunol 3: 733-740.
27. Macrae AI, Dutia BM, Milligan S, Brownstein DG, Allen DJ, et al. (2001) Analysis of a novel strain of murine gammaherpesvirus reveals a genomic locus important for acute pathogenesis. J Virol 75: 5315-5327.
28. Dutia BM, Clarke CJ, Allen DJ, Nash AA (1997) Pathological changes in the spleens of gamma interferon receptor- deficient mice infected with murine gammaherpesvirus: a role for CD8 T cells. Journal of Virology 71: 4278-4283.
29. Shariatmadari R, Sipila PP, Huhtaniemi IT, Poutanen M (2001) Improved technique for detection of enhanced green fluorescent protein in transgenic mice. Biotechniques 30: 1282-1285.
30. Dutia BM, Roy DJ, Ebrahimi B, Gangadharan B, Efstathiou S, et al. (2004) Identification of a region of the virus genome involved in murine gammaherpesvirus 68-induced splenic pathology. J Gen Virol 85: 1393-1400.
31. Adler H, Messerle M, Koszinowski UH (2001) Virus reconstituted from infectious bacterial artificial chromosome (BAC)-cloned murine gammaherpesvirus 68 acquires wild-type properties in vivo only after excision of BAC vector sequences. J Virol 75: 5692-5696.
32. Ebrahimi B, Dutia BM, Roberts KL, Garcia-Ramirez JJ, Dickinson P, et al. (2003) Transcriptome profile of murine gammaherpesvirus-68 lytic infection. J Gen Virol 84: 99-109.
33. Wakim LM, Jones CM, Gebhardt T, Preston CM, Carbone FR (2008) CD8(+) T-cell attenuation of cutaneous herpes simplex virus infection reduces the average viral copy number of the ensuing latent infection. Immunol Cell Biol 86: 666-675.
34. Sacher T, Podlech J, Mohr CA, Jordan S, Ruzsics Z, et al. (2008) The major virus-producing cell type during murine cytomegalovirus infection, the hepatocyte, is not the source of virus dissemination in the host. Cell Host Microbe 3: 263-272.
35. Clambey ET, Virgin HWt, Speck SH (2000) Disruption of the murine gammaherpesvirus 68 M1 open reading frame leads to enhanced reactivation from latency. Journal of Virology 74: 1973-1984.
36. Skelton D, Satake N, Kohn DB (2001) The enhanced green fluorescent protein (eGFP) is minimally immunogenic in C57BL/6 mice. Gene Ther 8: 1813-1814.
37. + T cells. Journal of Experimental Medicine 184: 863-871.