Brn3a as a marker of retinal ganglion cells: Qualitative and quantitative time course studies in naïve and optic nerve-injured retinas

Investigative Ophthalmology and Visual Science

Volumn 50, Issue 8, 2009, Pages 3860-3868

  Nadal Nicolás, Francisco M ^a,b   Jiménez López, Manuel ^a   Sobrado Calvo, Paloma ^a   Nieto López, Leticia ^a   Cánovas Martinez, Isabel ^a   Salinas Navarro, Manuel ^a   Vidal Sanz, Manuel ^a   Agudo, Marta ^a,b  

^a UNIVERSITY OF MURCIA   (Spain)

^b HOSPITAL UNIVERSITARIO VIRGEN DE LA ARRIXACA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; FLUOROGOLD; TRANSCRIPTION FACTOR POU4F1; 2 HYDROXY 4,4' DIAMIDINOSTILBENE, METHANESULFONATE SALT; 2-HYDROXY-4,4'-DIAMIDINOSTILBENE, METHANESULFONATE SALT; FLUORESCENT DYE; STILBAMIDINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL LOSS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; EX VIVO STUDY; FEMALE; IMMUNOREACTIVITY; ISOTOPE LABELING; NERVE CRUSH; NERVE TRANSECTION; NONHUMAN; OPTIC NERVE INJURY; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RAT; RETINA GANGLION CELL; RETINA INJURY; WESTERN BLOTTING; ANIMAL; CELL COUNT; FLUORESCENCE MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; METABOLISM; RETINA; SPRAGUE DAWLEY RAT; TIME;

ANIMALS; BIOLOGICAL MARKERS; BLOTTING, WESTERN; CELL COUNT; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; NERVE CRUSH; OPTIC NERVE INJURIES; RATS; RATS, SPRAGUE-DAWLEY; RETINA; RETINAL GANGLION CELLS; STILBAMIDINES; TIME FACTORS; TRANSCRIPTION FACTOR BRN-3A;

EID: 68349101301     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.08-3267     Document Type: Article

References (54)

1. Berkelaar M, Clarke DB, Wang YC, Bray GM, Aguayo AJ. Axotomy results in delayed death and apoptosis of retinal ganglion cells in adult rats. J Neurosci. 1994;14:4368-4374.
2. Chidlow G, Casson R, Sobrado-Calvo P, Vidal-Sanz M, Osborne NN. Measurement of retinal injury in the rat after optic nerve transection: an RT-PCR study. Mol Vis. 2005;11:387-396.
3. Lafuente MP, Villegas-Perez MP, Selles-Navarro I, Mayor-Torroglosa S, Miralles dl, Vidal-Sanz M. Retinal ganglion cell death after acute retinal ischemia is an ongoing process whose severity and duration depends on the duration of the insult. Neuroscience. 2002;109: 157-168.
4. Vidal-Sanz M, Lafuente M, Sobrado-Calvo P, et al. Death and neu-roprotection of retinal ganglion cells after different types of injury. Neurotox Res. 2000;2:215-227.
5. Vidal-Sanz M, Lafuente MP, Mayor S, de Imperial JM, Villegas-Perez MP. Retinal ganglion cell death induced by retinal ischemia: neu-roprotective effects of two alpha-2 agonists. Surv Ophthalmol. 2001;45(suppl 3)S261-S267.
6. Villegas-Perez MP, Vidal-Sanz M, Lund RD. Mechanism of retinal ganglion cell loss in inherited retinal dystrophy. Neuroreport. 1996;7:1995-1999.
7. Villegas-Perez MP, Vidal-Sanz M, Rasminsky M, Bray GM, Aguayo AJ. Rapid and protracted phases of retinal ganglion cell loss follow axotomy in the optic nerve of adult rats. JNeurobiol. 1993;24:23-36.
8. Parrilla-Reverter G, Agudo M, Sobrado-Calvo P, et al. Neuroprotec-tive effect of Neurotrophin4, Brain derived neurotrophic factor and ciliary neurotrophic factor against intraorbital nerve crush induced retinal ganglion cell loss: a quantitative in vivo study. Exp Eye Res. 2009. doi: 10.1016/j.exer.2009.02.015.
9. Bray GM, Villegas-Perez MP, Vidal-Sanz M, Aguayo AJ. The use of peripheral nerve grafts to enhance neuronal survival, promote growth and permit terminal reconnections in the central nervous system of adult rats. J Exp Biol. 1987;132:5-19.
10. Lafuente MP, Villegas-Perez MP, Sobrado-Calvo P, Garcia-Aviles A, Miralles dI, Vidal-Sanz M. Neuroprotective effects of alpha(2)-se-lective adrenergic agonists against ischemia-induced retinal ganglion cell death. Invest Ophthalmol Vis Sci. 2001;42:2074-2084.
11. Peinado-Ramon P, Salvador M, Villegas-Perez MP, Vidal-Sanz M. Effects of axotomy and intraocular administration of NT-4, NT-3, and brain-derived neurotrophic factor on the survival of adult rat retinal ganglion cells. a quantitative in vivo study. Invest Ophthal-mol Vis Sci. 1996;37:489-500.
12. Vidal-Sanz M, De la Villa P, Aviles-Trigueros M, et al. Neuroprotec-tion of retinal ganglion cell function and their central nervous system targets. Eye. 2007;21:S42-S45.
13. Villegas-Perez MP, Vidal-Sanz M, Bray GM, Aguayo AJ. Influences of peripheral nerve grafts on the survival and regrowth of axoto-mized retinal ganglion cells in adult rats. J Neurosci. 1988;8:265-280.
14. Drager UC, Olsen JF. Ganglion cell distribution in the retina of the mouse. Invest Ophthalmol Vis Sci. 1981;20:285-293.
15. Jeon CJ, Strettoi E, Masland RH. The major cell populations of the mouse retina. J Neurosci. 1998;18:8936-8946.
16. Perry VH. Evidence for an amacrine cell system in the ganglion cell layer of the rat retina. Neuroscience. 1981;6:931-944.
17. Perry VH, Henderson Z, Linden R. Postnatal changes in retinal ganglion cell and optic axon populations in the pigmented rat. J Comp Neurol. 1983;219:356-368.
18. Salinas-Navarro M, Mayor-Torroglosa S, Jimenez-Lopez M, et al. A computerized analysis of the entire retinal ganglion cell population and its spatial distribution in adult rats. Vision Res. 2009;49:115-126.
19. Thanos S, Vidal-Sanz M, Aguayo AJ. The use of rhodamine-B-iso-thiocyanate (RITC) as an anterograde and retrograde tracer in the adult rat visual system. Brain Res. 1987;406:317-321.
20. Vidal-Sanz M, Villegas-Perez MP, Bray GM, Aguayo AJ. Persistent retrograde labeling of adult rat retinal ganglion cells with the carbocyanine dye diI. Exp Neurol. 1988;102:92-101.
21. Danias J, Shen F, Kavalarakis M, et al. Characterization of retinal damage in the episcleral vein cauterization rat glaucoma model. Exp Eye Res. 2006;82:219-228.
22. Wang S, Villegas-Perez MP, Vidal-Sanz M, Lund RD. Progressive optic axon dystrophy and vascular changes in rd mice. Invest Ophthalmol Vis Sci. 2000;41:537-545.
23. Canola K, Angenieux B, Tekaya M, et al. Retinal stem cells transplanted into models of late stages of retinitis pigmentosa preferentially adopt a glial or a retinal ganglion cell fate. Invest Ophthalmol Vis Sci. 2007;48:446-454.
24. McKerracher L, Vallee RB, Aguayo AJ. Microtubule-associated protein 1A (MAP 1A) is a ganglion cell marker in adult rat retina. Vis Neurosci. 1989;2:349-356.
25. Barnstable CJ, Drager UC. Thy-1 antigen: a ganglion cell specific marker in rodent retina. Neuroscience. 1984;11:847-855.
26. Perry VH, Morris RJ, Raisman G. Is Thy-1 expressed only by ganglion cells and their axons in the retina and optic nerve? J Neurocytol. 1984;13:809-824.
27. Dabin I, Barnstable CJ. Rat retinal Muller cells express Thy-1 following neuronal cell death. Glia. 1995;14:23-32.
28. Huang W, Fileta J, Guo Y, Grosskreutz CL. Downregulation of Thy1 in retinal ganglion cells in experimental glaucoma. Curr Eye Res. 2006;31:265-271.
29. Schlamp CL, Johnson EC, Li Y, Morrison JC, Nickells RW. Changes in Thy1 gene expression associated with damaged retinal ganglion cells. Mol Vis. 2001;7:192-201.
30. Bernstein SL, Koo JH, Slater BJ, Guo Y, Margolis FL. Analysis of optic nerve stroke by retinal Bex expression. Mol Vis. 2006;12: 147-155.
31. Surgucheva I, Weisman AD, Goldberg JL, Shnyra A, Surguchov A. Gamma-synuclein as a marker of retinal ganglion cells. Mol Vis. 2008;14:1540-1548.
32. Feng G, Mellor RH, Bernstein M, et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron. 2000;28:41-51.
33. Raymond ID, Vila A, Huynh UC, Brecha NC. Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina. Mol Vis. 2008;14:1559-1574.
34. Wang SW, Mu X, Bowers WJ, et al. Brn3b/Brn3c double knockout mice reveal an unsuspected role for Brn3c in retinal ganglion cell axon outgrowth. Development. 2002;129:467-477.
35. Buckingham BP, Inman DM, Lambert W, et al. Progressive ganglion cell degeneration precedes neuronal loss in a mouse model of glaucoma. J Neurosci. 2008;28:2735-2744.
36. Quina LA, Pak W, Lanier J, et al. Brn3a-expressing retinal ganglion cells project specifically to thalamocortical and collicular visual pathways. J Neurosci. 2005;25:11595-11604.
37. Johnson TV, Martin KR. Development and characterization of an adult retinal explant organotypic tissue culture system as an in vitro intraocular stem cell transplantation model. Invest Ophthal-mol Vis Sci. 2008;49:3503-3512.
38. Agudo M, Perez-Marin M, Lonngren U, et al. Time course profiling of the retinal transcriptome after optic nerve transection and optic nerve crush. Mol Vis. 2008;14:1050-1063.
39. Agudo M, Perez-Marin MC, Sobrado-Calvo P, et al. Immediate upregulation of proteins belonging to different branches of the apoptotic cascade in the retina after optic nerve transection and optic nerve crush. Invest Ophthalmol Vis Sci. 2009;50:424-431.
40. Vidal-Sanz M, Bray GM, Villegas-Perez MP, Thanos S, Aguayo AJ. Axonal regeneration and synapse formation in the superior col-liculus by retinal ganglion cells in the adult rat. J Neurosci. 1987; 7:2894-2909.
41. Salinas-Navarro M, Jimenez-Lopez M, Valiente-Soriano F, et al. Retinal ganglion cell population in adult albino and pigmented mice: a computerised analysis of the entire population and its spatial distribution. Vision Res. 2009;49:637-649.
42. Lafuente MP, Villegas-Perez MP, Mayor S, Aguilera ME, Miralles dI, Vidal-Sanz M. Neuroprotective effects of brimonidine against transient ischemia-induced retinal ganglion cell death: a dose response in vivo study. Exp Eye Res. 2002;74:181-189.
43. Kobbert C, Apps R, Bechmann I, Lanciego JL, Mey J, Thanos S. Current concepts in neuroanatomical tracing. Prog Neurobiol. 2000;62:327-351.
44. Mansour-Robaey S, Clarke DB, Wang YC, Bray GM, Aguayo AJ. Effects of ocular injury and administration of brain-derived neuro-trophic factor on survival and regrowth of axotomized retinal ganglion cells. Proc Natl Acad Sci USA. 1994;91:1632-1636.
45. Xiang M, Zhou L, Macke JP, et al. The Brn-3 family of POU-domain factors: primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons. J Neu-rosci. 1995;15:4762-4785.
46. Drager UC, Hofbauer A. Antibodies to heavy neurofilament subunit detect a subpopulation of damaged ganglion cells in retina. Nature. 1984;309:624-626.
47. Levkovitch-Verbin H, Quigley HA, Martin KR, Zack DJ, Pease ME, Valenta DF. A model to study differences between primary and secondary degeneration of retinal ganglion cells in rats by partial optic nerve transection. Invest Ophthalmol Vis Sci. 2003;44: 3388-3393.
48. Fileta JB, Huang W, Kwon GP, et al. Efficient estimation of retinal ganglion cell number: a stereological approach. J Neurosci Methods. 2008;170:1-8.
49. Ko ML, Hu DN, Ritch R, Sharma SC, Chen CF. Patterns of retinal ganglion cell survival after brain-derived neurotrophic factor administration in hypertensive eyes of rats. Neurosci Lett. 2001;305: 139-142.
50. Siu AW, Leung MC, To CH, Siu FK, Ji JZ, So KF. Total retinal nitric oxide production is increased in intraocular pressure-elevated rats. Exp Eye Res. 2002;75:401-406.
51. Budram-Mahadeo V, Morris PJ, Latchman DS. The Brn-3a transcription factor inhibits the pro-apoptotic effect of p53 and enhances cell cycle arrest by differentially regulating the activity of the p53 target genes encoding Bax and p21(CIP1/Waf1). Oncogene. 2002; 21:6123-6131.
52. Hudson CD, Morris PJ, Latchman DS, Budhram-Mahadeo VS. Brn-3a transcription factor blocks p53-mediated activation of proapo-ptotic target genes Noxa and Bax in vitro and in vivo to determine cell fate. J Biol Chem. 2005;280:11851-11858.
53. Latchman DS. The Brn-3a transcription factor. Int J Biochem Cell Biol. 1998;30:1153-1157.
54. Farooqui-Kabir SR, Budhram-Mahadeo V, Lewis H, Latchman DS, Marber MS, Heads RJ. Regulation of Hsp27 expression and cell survival by the POU transcription factor Brn3a. Cell Death Differ. 2004;11:1242-1244