Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana

Journal of Biomolecular Techniques

Volumn 20, Issue 2, 2009, Pages 96-100

  Hart, Shanion M ^a   Basu, Chhandak ^a  

^a UNIVERSITY OF NORTHERN COLORADO   (United States)

Author keywords

Arabidopsis thaliana; Digoxigenin; Gene detection; Nonradioactive

Indexed keywords

DIGOXIGENIN; ELONGATION FACTOR 1; NUCLEIC ACID; PLASMID DNA; PLANT DNA;

ARABIDOPSIS; ARTICLE; DNA HYBRIDIZATION; ENVIRONMENTAL FACTOR; GENE PROBE; GENE TARGETING; GENETIC ANALYSIS; HOUSEKEEPING GENE; IMMUNOASSAY; IN SITU HYBRIDIZATION; NONHUMAN; PLANT BIOTECHNOLOGY; PLANT GENE; DNA PROBE; GENETICS; METHODOLOGY; PLANT GENOME; PLASMID; SENSITIVITY AND SPECIFICITY;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

ARABIDOPSIS; DIGOXIGENIN; DNA PROBES; DNA, PLANT; GENES, PLANT; GENOME, PLANT; IMMUNOASSAY; IN SITU HYBRIDIZATION; PEPTIDE ELONGATION FACTOR 1; PLASMIDS; SENSITIVITY AND SPECIFICITY;

EID: 68249161338     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Shu GG, Baum DA, Mets LJ. Detection of gene expression patterns in various plant tissues using non-radioactive mRNA in situ hybridization. The World Wide Web Journal of Biology 1999;4:1-4. http://epress.com/w3jbio/vol4/ shu/index.html
2. McCabe MS, Power JB, de Laat AdMM, Davey MR. Detection of single-copy genes in DNA from transgenic plants by nonradioactive Southern blot analysis. Mol Biotechnol 1997;7:79-84.
3. Dietzgen RG, Abedina M, Higgins CM, Karunatratne S, Vickers J. Nonradioactive detection of transgenes in plant genomic Southern Blots. Biochemica 1999;1:19-20.
4. Neuhaus-Url G, Neuhaus G. The use of nonradioactive digoxigenin chemiluminescent technology for plant genomic Southern blot hybridization: A comparison with radioactivity. Transgenic Res 1993;2:115-120.
5. Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975;98:503-517.
6. Van Sandt VS, Guisez Y, Verbelen JP, Vissenberg K. Xyloglucan endotransglycosylase/hydrolase (XTH) is encoded by a multigene family in the primitive vascular land plant Selaginella kraussiana. Plant Biol 2007;9:142-146.
7. Shirasawa K, Shiokai S, Yamaguchi M, Kishitani S, Nishio T. Dot-blot-SNP analysis for practical plant breeding and cultivar identification in rice. Theor Appl Genet 2006;113:147-155.
8. Adhami F, Müller S, Hauser MT. Nonradioactive labeling of large DNA fragments for genome walking, RFLP and northern blot analysis. Biotechniques 1999;27:314-320.
9. Lahaye T, Rueger B, Toepsch S, Thalhammer J, Schulze-Lefert P. Detection of single-copy sequences with digoxigenin-labeled probes in a complex plant genome after separation on pulsed-field gels. Biotechniques 1996;21:1067-1070.
10. Hilario E, Bennell TF, Rikkerink E. Screening a BAC library with nonradioactive overlapping oligonucleotide (overgo) probes. In Hilario E, Mackay J. (eds): Protocols for Nucleic Acid Analysis by Nonradioactive Probes, 2nd ed, vol 353. New York: Humana Press, 2007:79-91.
11. Nicot N, Hausman J-F, Hoffmann L, Evers D. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot 2005;56:2907-2914.
12. Dube A, Thai S, Gaspar J, Rudders S, Libermann TA, Iruela-Arispe L, Oettgen P. ELF-1 is a transcriptional regulator of the Tie2 gene during vascular development. Circulation Res 2001;88:237-244.