A versatile mesoindentation system to evaluate the micromechanical properties of soft, hydrated substrates on a cellular scale

Journal of Biomedical Materials Research - Part A

Volumn 90, Issue 4, 2009, Pages 1206-1217

  Saxena, Tarun ^a   Gilbert, Jeremy L ^a   Hasenwinkel, Julie M ^a  

^a Syracuse University   (United States)

Author keywords

Agarose; Microindentation; Poly(2 hydroxyethyl methacrylate); Spinal cord tissue; Viscoelasticity; Young's modulus

Indexed keywords

AGAROSE; MICROINDENTATION; POLY(2-HYDROXYETHYL METHACRYLATE); SPINAL CORD TISSUE; YOUNG'S MODULUS;

BIOMECHANICS; DOSIMETRY; ELASTIC MODULI; ELASTICITY; HYDRATES; HYDRATION; HYDROGELS; LABELS; MECHANICAL PROPERTIES; POLYMERS; RESIDUAL STRESSES; SALINE WATER; STRESS RELAXATION; TISSUE; VISCOELASTICITY; VISCOSITY; WATER CONTENT;

SUBSTRATES;

AGAROSE; POLYMACON; POLYMER;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; HYDROGEL; NONHUMAN; RAT; SPINAL CORD; VALIDATION PROCESS; VISCOELASTICITY; WATER FLOW;

ANIMALS; BIOMECHANICS; HYDROGELS; METHACRYLATES; RATS; SPINAL CORD; WATER;

EID: 68249154757     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.32178     Document Type: Article

References (34)

1. Discher DE, Janmey P, Wang YL. Tissue cells feel and respond to the stiffness of their substrate. Science 2005;310:1139-1143.
2. Georges PC, Janmey PA. Cell type-specific response to growth on soft materials. J Appl Physiol 2005;98:1547-1553.
3. Pelham RJ Jr, Wang YL. Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci USA 1998;94:1361-1365.
4. Gray DS, Tien J, Chen CS. Repositioning of cells by mechanotaxis on surfaces with micropatterned Young's modulus. J Biomed Mater Res A 2003;66:605-614.
5. Ebenstein DM, Pruitt LA. Nanoindentation of soft hydrated materials for application to vascular tissues. J Biomed Mater Res A 2004;69:222-232.
6. Balgude AP, Yu X, Szymanski A, Bellamkonda RV. Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures. Biomaterials 2001;22:1077-1084.
7. Georges PC, Miller WJ, Meaney DF, Sawyer ES, Janmey PA. Matrices with compliance comparable to that of brain tissue select neuronal over glial growth in mixed cortical cultures. Biophys J 2006;90:3012-3018.
8. Ozawa H, Matsumoto T, Ohashi T, Sato M, Kokubun S. Comparison of spinal cord gray matter and white matter softness: Measurement by pipette aspiration method. J Neurosurg 2001;95:221-224.
9. Abé H, Hayashi K, Sato M. Data Book on Mechanical Properties of Living Cells, Tissues, and Organs. Tokyo: Springer-Verlag; 1996.
10. Anseth KS, Bowman CN, Brannon-Peppas L. Mechanical properties of hydrogels and their experimental determination. Biomaterials 1996;17:1647-1657.
11. Jacot JG, Dianis S, Schnall J, Wong JY. A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues. J Biomed Mater Res A 2006;79:485-494.
12. Gilbert JL, Merkhan IK. Rate effects on the microindentation-based mechanical properties of oxidized, crosslinked, and highly crystalline ultrahigh-molecular-weight polyethylene. J Biomed Mater Res A 2005;71:549-558.
13. Gilbert JL, Cumber J, Butterfield A. Surface micromechanics of ultrahigh molecular weight polyethylene: Microindentation testing, crosslinking, and material behavior. J Biomed Mater Res 2002;61:270-281.
14. Wernle JD, Gilbert JL. Micromechanics of shelf-aged and retrieved UHMWPE tibial inserts: Indentation testing, oxidative profiling, and thickness effects. J Biomed Mater Res B Appl Biomater 2005;75:113-121.
15. Carone TW, Hasenwinkel JM. Mechanical and morphological characterization of homogeneous and bilayered poly(2-hydroxyethyl methacrylate) scaffolds for use in CNS nerve regeneration. J Biomed Mater Res B 2006;78:274-282.
16. Carone TW, Pinder BP, Ray BR, Hasenwinkel JM. Micromechanical and morphological analysis of macroporous poly(2-hydroxyethyl methacrylate) hydrogels. Society of Biomaterials, 29th Annual Meeting, Reno, NV, 2003.
17. Ahearne M, Yang Y, El Haj AJ, Then KY, Liu KK. Characterizing the viscoelastic properties of thin hydrogel-based constructs for tissue engineering applications. J R Soc Interface 2005;2:455-463.
18. Oakland RJ, Hall RM, Wilcox RK, Barton DC. The biomechanical response of spinal cord tissue to uniaxial loading. Proc Inst Mech Eng [H] 2006;220:489-492.
19. Fiford RJ, Bilston LE. The mechanical properties of rat spinal cord in vitro. J Biomech 2005;38:1509-1515.
20. Sneddon IN. The relation between load and penetration in the axisymmetric Boussinesq problem for a punch of arbitrary profile. Int J Eng Sci 1965;3:47-57.
21. Normand V, Lootens DL, Amici E, Plucknett KP, Aymard P. New insight into agarose gel mechanical properties. Biomacromolecules 2000;1:730-738.
22. Stolz M, Raiteri R, Daniels AU, VanLandingham MR, Baschong W, Aebi U. Dynamic elastic modulus of porcine articular cartilage determined at two different levels of tissue organization by indentation-type atomic force microscopy. Biophys J 2004;86:3269-3283.
23. Fung YC. Biomechanics, 2nd ed. New York: Springer-Verlag; 1993.
24. Provenzano PP, Lakes RS, Corr DT. Application of nonlinear viscoelastic models to describe ligament behavior. Biomech Model Mechanobiol 2002;1:45-57.
25. Rico F, Roca-Cusachs P, Gavara N, Farré R, Rotger M, Navajas D. Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips. Phys Rev E Stat Nonlin Soft Matter Phys 2005;72:219-229.
26. Dong R, Jensen TW, Engberg K, Nuzzo RG, Leckband DE. Variably elastic hydrogel patterned via capillary action in microchannels. Langmuir 2007;23:1483-1488.
27. Nitta T, Haga H, Kawabata K, Abe K, Sambongi T. Comparing microscopic with macroscopic elastic properties of polymer gel. Ultramicroscopy 2000;82:223-226.
28. Eldridge JI, Zhu D, Miller RA. Mesoscopic nonlinear elastic modulus of thermal barrier coatings determined by cylindrical punch indentation. J Am Ceram Soc 2001;84:2737-2739.
29. Aymard P, Martin DR, Plucknett K, Foster TJ, Clark AH, Norton IT. Influence of thermal history on the structural and mechanical properties of agarose gels. Biopolymers 2001;59:131-144.
30. Scandiucci de Freitas P, Wirz D, Stolz M, Göpfert B, Friederich NF, Daniels AU. Pulsatile dynamic stiffness of cartilagelike materials and use of agarose gels to validate mechanical methods and models. J Biomed Mater Res B 2006;78:347-357.
31. Meyers A, Chawla KK. Mechanical Behavior of Materials. Englewood Cliffs, NJ: Prentice-Hall; 1999.
32. Bakshi A, Fisher O, Dagci T, Himes BT, Fischer I, Lowman A. Mechanically engineered hydrogel scaffolds for axonal growth and angiogenesis after transplantation in spinal cord injury. J Neurosurg Spine 2002;3:322-329.
33. Dalton PD, Flynn L, Shoichet MS. Manufacture of poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) hydrogel tubes for use as nerve guidance channels. Biomaterials 2002;23:3843-3851.
34. Williams G, Watts DC. Non-symmetrical dielectric relaxation behaviour arising from a simple empirical decay function. Trans Faraday Soc 1970;66:80-85.