Functional importance of GGXG sequence motifs in putative reentrant loops of 2HCT and ESS transport proteins

Biochemistry

Volumn 48, Issue 31, 2009, Pages 7448-7456

  Dobrowolski, Adam ^a   Lolkema, Juke S ^a,b  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b University of Groningen Center of Biological Sciences Haren   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ACETAMIDO; AMINO ACID SEQUENCE; CITRATE TRANSPORTERS; CYSTEINE-SCANNING MUTAGENESIS; CYTOPLASMIC LOOPS; DATA SUPPORT; DISULFONIC ACID; GLUTAMATE TRANSPORTERS; MEMBRANE-PERMEABLE; N-TERMINALS; RESIDUAL ACTIVITY; SEQUENCE MOTIFS; STRUCTURAL MODELS; THIOL REAGENTS; TRANSMEMBRANE SEGMENTS; TRANSPORT ACTIVITY; TRANSPORT PROTEINS; TWO-DOMAIN STRUCTURES; WILD TYPES;

AMINES; AMINO ACIDS; CELL MEMBRANES; DIELECTRIC DEVICES; MODEL STRUCTURES; ORGANIC ACIDS; PROTEIN FOLDING; SODIUM; SUGAR (SUCROSE);

MEMBRANES;

2 HYDROXYCARBOXYLATE TRANSPORTER; 4 ACETAMIDO 4' ISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; CARRIER PROTEIN; CITRIC ACID; GLUTAMATE SODIUM SYMPORTER; GLUTAMIC ACID; N ETHYLMALEIMIDE; PROTEIN CITS; PROTEIN GLTS; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MOTIF; SODIUM TRANSPORT; STRUCTURE ACTIVITY RELATION;

AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; AMINO ACID TRANSPORT SYSTEMS, ACIDIC; BACTERIAL PROTEINS; CARBOXYLIC ACIDS; CARRIER PROTEINS; ESCHERICHIA COLI PROTEINS; GLUTAMATE PLASMA MEMBRANE TRANSPORT PROTEINS; GLYCINE; MEMBRANE TRANSPORT PROTEINS; MODELS, MOLECULAR; PROTEIN STRUCTURE, TERTIARY; SYMPORTERS;

EID: 68249106068     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9004914     Document Type: Article

References (28)

1. Saier, M. H.Jr. (2000) A functional-phylogenetic classification system for transmembrane solute transporters. Microbiol. Mol. Rev. 64, 354-411.
2. Lolkema, J. S., and Slotboom, D. J. (1998) Estimation of structural similarity of membrane proteins by hydropathy profile alignment. Mol. Membr. Biol. 15, 33-42.
3. Lolkema, J. S., and Slotboom, D. J. (1998) Hydropathy profile alignment. A tool to search for structural homologues of membrane proteins. FEMS Microbiol. Rev. 22, 305-322.
4. Lolkema, J. S., and Slotboom, D. J. (2003) Classification of 29 families of secondary transport proteins. J. Mol. Biol. 327, 901-909.
5. Lolkema, J. S., and Slotboom, D. J. (2005) Sequence and hydropathy profile analysis of two classes of secondary transporters. Mol. Membr. Biol. 22, 177-189.
6. --dependent neurotransmitter transporters. Nature 437, 215-223.
7. +/sugar symport. Science 321, 810-814.
8. Weyand, S., Shimamura, T., Yajima, S., Suzuki, S., Mirza, O., Krusong, K., Carpenter, E. P., Rutherford, N. G., Hadden, J. M., O'Reilly, J., Ma, P., Saidijam, M., Patching, S. G., Hope, R. J., Norbertczak, H. T., Roach, P. C., Iwata, S., Henderson, P. J., and Cameron, A. D. (2008) Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter. Science 322, 708-713.
9. +-leicine transporters. Mol. Membr. Biol. 25, 567-570.
10. +-Glutamate Transporter GltS. Biochemistry 46, 2326-2332.
11. Sobczak, I., and Lolkema, J. S. (2005) The 2-hydroxycarboxylate transporter family: Physiology, structure, and mechanism. Microbiol. Mol. Biol. Rev. 69, 665-695.
12. Kalman, M., Gentry, D. R., and Cashel, M. (1991) Characterization of the Escherichia coli K12 gltS glutamate permease gene. Mol. Gen. Genet. 225, 379-386.
13. +/glutamate symport carrier gene of Escherichia coli B. J. Biol. Chem. 265, 21704-21708.
14. Krogh, A., Larsson, B., von Heijne, G., and Sonnhammer, E. L. (2001) Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes. J. Mol. Biol. 305, 567-580.
15. Lolkema, J. S., Sobczak, I., and Slotboom, D. J. (2005) Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops. FEBS J. 272, 2334-2344. (Pubitemid 40646801)
16. Lolkema, J. S. (2006) Domain structure and pore-loops in the 2- hydroxycarboxylate transporter family. J. Mol. Microbiol. Biotechnol. 11, 318-325.
17. Fu, D., Libson, A., Miercke, L. J., Weitzman, C., Nollert, P., Krucinski, J., and Stroud, R. M. (2000) Structural determinants of water permeation through aquaporin-1. Science 290, 481-486.
18. Khademi, S., O'Connell, J.III, Remis, J., Robles-Colmenares, Y., Miercke, L. J. W., and Stroud, R. (2004) Mechanism of ammonia transport by Amt/MEP/Rh: Structure of AmtB at 1.35Å. Science 305, 1587-1594. (Pubitemid 39296348)
19. Van den Berg, B., Clemons, W. M.Jr., Collinson, I., Modis, Y., Hartmann, E., Harrison, S. C., and Rapoport, T. A. (2004) X-ray structure of a protein-conducting channel. Nature 427, 24-26.
20. + antiporter and insights into mechanism of action and regulation by pH. Nature 435, 1197-1202.
21. Kaback, H. R. (1974) Transport in isolated bacterial membrane vesicles. Methods Enzymol. 31, 698-709.
22. Konings, W. N., Barnes, E. N.Jr., and Kaback, H. R. (1971) Mechanisms of active transport in isolated membrane vesicles. 2. The coupling of reduced phenazine methosulfate to the concentrative uptake of β-galactosides and amino acids. J. Biol. Chem. 246, 5857-5861.
23. Sobczak, I., and Lolkema, J. S. (2003) Accessibility of cysteine residues in a cytoplasmic loop of CitS of Klebsiella pneumoniae is controlled by the catalytic state of the transporter. Biochemistry 42, 9789-9796. (Pubitemid 36993152)
24. +-citrate transporter CitS of Klebsiella pneumoniae. J. Biol. Chem. 279, 31113-31120.
25. Deguchi, Y., Yamato, I., and Anraku, Y. (1989) Molecular cloning of gltS and gltP, which encode glutamate carriers of Escherichia coli B. J. Bacteriol. 171, 1314-1319. (Pubitemid 19080767)
26. +-dependent citrate carrier of Klebsiella pneumoniae: High level expression and site directed mutagenesis of asparagine-185 and glutamate- 195. Arch. Microbiol. 174, 67-73.
27. Yernool, D., Boudker, O., Jin, Y., and Gouaux, E. (2004) Structure of a glutamate transporter homologue from Pyrococcus horikoshii. Nature 431, 811-818. (Pubitemid 39434071)
28. Slotboom, D.-J., Sobczak, I., Konings, W. N., and Lolkema, J. S. (1999) A conserved serine-rich stretch in the glutamate transporter family forms a substrate-sensitive re-entrant loop. Proc. Natl. Acad. Sci. U.S.A. 96, 14282-14287.