Enhanced enrichment and detection of thermotolerant Campylobacter species from water using the Portable Microbe Enrichment Unit and real-time PCR

Canadian Journal of Microbiology

Volumn 55, Issue 7, 2009, Pages 849-858

  Pitkänen, Tarja ^a   Bräcker, Juliane ^a,b   Miettinen, Ilkka T ^a   Heitto, Anneli ^c   Pesola, Jouni ^c,d,e   Hakalehto, Elias ^c,d  

^a NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

^b UNIVERSITY OF DUISBURG ESSEN   (Germany)

^c Finnoflag Oy ^*  (Finland)

^d UNIVERSITY OF EASTERN FINLAND   (Finland)

^e KUOPIO UNIVERSITY HOSPITAL   (Finland)

Author keywords

Campylobacter; Enrichment; Portable microbe enrichment unit; Real time PCR; Water

Indexed keywords

CAMPYLOBACTER; CAMPYLOBACTER JEJUNI; CAMPYLOBACTER SPECIES; CAMPYLOBACTERS; COLONY COUNTS; DETECTION TIME; DRINKING WATER; ENRICHMENT; ENRICHMENT UNITS; INTERNATIONAL STANDARDS; MICROAEROBICS; PORTABLE MICROBE ENRICHMENT UNIT; REAL-TIME PCR; THERMOTOLERANT;

POTABLE WATER;

DRINKING WATER;

BACTERIUM; DRINKING WATER; MOTILITY; OXIC CONDITIONS; REAL TIME; SPECIES DIVERSITY; STANDARDIZATION; SURVEYING; WATER QUALITY;

ARTICLE; BACTERIAL CELL; BACTERIAL COUNT; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; CAMPYLOBACTER; CAMPYLOBACTER JEJUNI; DEVICE; ENRICHMENT CULTURE; HEAT TOLERANCE; INOCULATION; MICROBIOLOGICAL EXAMINATION; PORTABLE MICROBE ENRICHMENT UNIT; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; WATER CONTAMINATION; WATER SAMPLING;

BACTERIOLOGICAL TECHNIQUES; CAMPYLOBACTER; FRESH WATER; POLYMERASE CHAIN REACTION; TEMPERATURE;

CAMPYLOBACTER; CAMPYLOBACTER JEJUNI;

EID: 68149116262     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W09-040     Document Type: Article

References (28)

1. Abu-Halaweh, M., Bates, J., and Patel, B.K.C. 2005. Rapid detection and differentiation of pathogenic Campylobacter jejuni and Campylobacter coli by real-time PCR. Res. Microbiol. 156: 107-114. doi:10.1016/j.resmic.2004.08.008. PMID:15636755.
2. Abulreesh, H.H., Paget, T.A., and Goulder, R. 2005. Recovery of thermophilic campylobacters from pond water and sediment and the problem of interference by background bacteria in enrichment culture. Water Res. 39: 2877-2882. doi:10.1016/j.watres. 2005.05.004. PMID:15979120. (Pubitemid 41044163)
3. Abulreesh, H.H., Paget, T.A., and Goulder, R. 2006. Campylobacter in waterfowl and aquatic environments: Incidence and methods of detection. Environ. Sci. Technol. 40: 7122-7131. doi:10. 1021/es060327l. PMID:17180958.
4. Bang, D.D., Wedderkopp, A., Pedersen, K., and Madsen, M. 2002. Rapid PCR using nested primers of the 16S rRNA and the hippuricase (hipO) genes to detect Campylobacter jejuni and Campylobacter coli in environmental samples. Mol. Cell. Probes, 16: 359-369. doi:10.1006/mcpr.2002.0434. PMID:12477440.
5. Butzler, J.P. 2004. Campylobacter, from obscurity to celebrity. Clin. Microbiol. Infect. 10: 868-876. doi:10.1111/j.1469-0691. 2004.00983.x. PMID:15373879.
6. Cook, N. 2003. The use of NASBA for the detection of microbial pathogens in food and environmental samples. J. Microbiol. Methods, 53: 165-174. doi:10.1016/S0167-7012(03)00022-8. PMID:12654488.
7. Cools, I., Uyttendaele, M., D'Haese, E., Nelis, H.J., and Debevere, J. 2006. Development of a real-time NASBA assay for the detection of Campylobacter jejuni cells. J. Microbiol. Methods, 66: 313-320. doi:10.1016/j.mimet.2005.12. 004. PMID: 16443295.
8. Engvall, E.O., Brandstrom, B., Gunnarsson, A., Morner, T., Wahlstrom, H., and Fermer, C. 2002. Validation of a polymerase chain reaction/restriction enzyme analysis method for species identification of thermophilic campylobacters isolated from domestic and wild animals. J. Appl. Microbiol. 92: 47-54. doi:10.1046/j.1365-2672.2002.01491.x. PMID:11849327.
9. Fermer, C., and Engvall, E.O. 1999. Specific PCR identification and differentiation of the thermophilic campylobacters, Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis. J. Clin. Microbiol. 37: 3370-3373. PMID:10488210.
10. Hakalehto, E., Pesola, J., Heitto, L., Narvanen, A., and Heitto, A. 2007. Aerobic and anaerobic growth modes and expression of type 1 fimbriae in Salmonella. Pathophysiology, 14(1): 61-69. doi:10.1016/j.pathophys.2007.01.003. PMID:17434297. (Pubitemid 46636229)
11. Hakalehto, E., Humppi, T., and Paakkanen, H. 2008. Dualistic acidic and neutral glucose fermentation balance in small intestine: simulation in vitro. Pathophysiology, 15(4): 211-220. doi:10.1016/j.pathophys.2008.07.001. PMID:18804970.
12. Heisick, J., Lanier, J., and Peeler, J.T. 1984. Comparison of enrichment methods and atmosphere modification procedures for isolating Campylobacter jejuni from foods. Appl. Environ. Microbiol. 48: 1254-1255. PMID:6393876.
13. Hernandez, J., Alonso, J.L., Fayos, A., Amoros, I., and Owen, R.J. 1995. Development of a PCR assay combined with a short enrichment culture for detection of Campylobacter jejuni in estuarine surface waters. FEMS Microbiol. Lett. 127: 201-206. doi:10. 1111/j.1574-6968.1995.tb07474.x. PMID:7758934.
14. Hrudey, S.E., and Hrudey, E.J. 2007. Published case studies of waterborne disease outbreaks - evidence of a recurrent threat. Water Environ. Res. 79: 233-245. doi:10.2175/ 106143006X95483. PMID:17469655.
15. ISO 17995. 2005. Water quality. Detection and enumeration of thermotolerant Campylobacter species. International Organization for Standardization (ISO), Geneva, Switzerland.
16. ISO 19458. 2006. Water quality. Sampling for microbiological analysis. International Organization for Standardization (ISO), Geneva, Switzerland.
17. Josefsen, M.H., Jacobsen, N.R., and Hoorfar, J. 2004. Enrichment followed by quantitative PCR both for rapid detection and as a tool for quantitative risk assessment of food-borne thermotolerant campylobacters. Appl. Environ. Microbiol. 70: 3588-3592. doi:10.1128/AEM.70.6.3588-3592.2004. PMID:15184161.
18. Lehtola, M.J., Pitkänen, T., Miebach, L., and Miettinen, I.T. 2006. Survival of Campylobacter jejuni in potable water biofilms: a comparative study with different detection methods. Water Sci. Technol. 54: 57-61. doi:10.2166/wst.2006.448. PMID: 17037133.
19. Moore, J., Caldwell, P., and Millar, B. 2001. Molecular detection of Campylobacter spp. in drinking, recreational and environmental water supplies. Int. J. Hyg. Environ. Health, 204: 185-189. doi:10.1078/1438-4639-00096. PMID:11759163.
20. Moreno, Y., Botella, S., Alonso, J.L., Ferrus, M.A., Hernandez, M., and Hernandez, J. 2003. Specific detection of Arcobacter and Campylobacter strains in water and sewage by PCR and fluorescent in situ hybridization. Appl. Environ. Microbiol. 69: 1181- 1186. doi:10.1128/AEM.69.2.1181-1186.2003. PMID:12571045.
21. Nam, H.M., Srinivasan, V., Murinda, S.E., and Oliver, S.P. 2005. Detection of Campylobacter jejuni in dairy farm environmental samples using SYBR Green real-time polymerase chain reaction. Foodborne Pathog. Dis. 2: 160-168. doi:10.1089/fpd.2005.2.160. PMID:15992311.
22. Neumann, N.F., Smith, D.W., and Belosevic, M. 2005. Waterborne disease: An old foe re-emerging? J. Environ. Eng. Sci. 4: 155-171. doi:10.1139/s04-061.
23. Perelle, S., Josefsen, M., Hoorfar, J., Dilasser, F., Grout, J., and Fach, P. 2004. A LightCycler real-time PCR hybridization probe assay for detecting food-borne thermophilic Campylobacter. Mol. Cell. Probes, 18: 321-327. doi:10.1016/j.mcp.2004.04.005. PMID:15294320. (Pubitemid 39061038)
24. Pitkänen, T., Miettinen, I.T., Nakari, U.-M., Takkinen, J., Nieminen, K., Siitonen, A., et al. 2008. Faecal contamination of a municipal drinking water distribution system in association with Campylobacter jejuni infections. J. Water Health, 6(3): 365-376. doi:10.2166/wh.2008.050. PMID:19108557.
25. Sails, A.D., Bolton, F.J., Fox, A.J., Wareing, D.R.A., and Greenway, D.L.A. 2002. Detection of Campylobacter jejuni and Campylobacter coli in environmental waters by PCR enzymelinked immunosorbent assay. Appl. Environ. Microbiol. 68: 1319-1324. doi:10.1128/AEM.68.3.1319-1324.2002. PMID: 11872483.
26. Sails, A.D., Fox, A.J., Bolton, F.J., Wareing, D.R., and Greenway, D.L. 2003. A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture. Appl. Environ. Microbiol. 69: 1383-1390. doi:10.1128/AEM.69.3.1383-1390. 2003. PMID:12620820.
27. Waage, A.S., Vardund, T., Lund, V., and Kapperud, G. 1999. Detection of small numbers of Campylobacter jejuni and Campylobacter coli cells in environmental water, sewage, and food samples by a seminested PCR assay. Appl. Environ. Microbiol. 65: 1636-1643. PMID:10103261.
28. Yang, C., Jiang, Y., Huang, K., Zhu, C., and Yin, Y. 2003. Application of real-time PCR for quantitative detection of Campylobacter jejuni in poultry, milk, and environmental water. FEMS Immunol. Med. Microbiol. 38: 265-271. doi:10.1016/S0928- 8244(03)00168-178 PMID:14522462.