Rapid PCR using nested primers of the 16s rRNA and the hippuricase (hipO) genes to detect Campylobacter jejuni and Campylobacter coli in environmental samples

Molecular and Cellular Probes

Volumn 16, Issue 5, 2002, Pages 359-369

  Bang, D D ^a   Wedderkopp, A ^a   Pedersen, K ^a   Madsen, M ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Broilers; Campylobacter detection; Campylobacter spp.; Chicken; Environmental sample; Nested PCR; PCR detection

Indexed keywords

AMINOACYLASE; PRIMER RNA; RIBOSOME RNA; AMIDASE; AMINOACYLASE I; BACTERIAL DNA; PRIMER DNA; RNA 16S;

ACCURACY; ARTICLE; ASSAY; BACTERIUM CULTURE; BACTERIUM DETECTION; CAMPYLOBACTER; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; COLONY FORMING UNIT; CULTURE MEDIUM; NONHUMAN; POLYMERASE CHAIN REACTION; POULTRY FARMING; PRIORITY JOURNAL; ANIMAL; BIRD DISEASE; CHICKEN; ENVIRONMENT; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; SENSITIVITY AND SPECIFICITY; STANDARD; TIME;

CAMPYLOBACTER; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; GALLUS GALLUS;

AMIDOHYDROLASES; ANIMALS; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; CHICKENS; DNA PRIMERS; DNA, BACTERIAL; ENVIRONMENT; POLYMERASE CHAIN REACTION; POULTRY DISEASES; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 0036812513     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.2002.0434     Document Type: Article

References (46)

1. Oberhelman, R. A. & Taylor, D. N. (2000). Campylobacter infections in developing countries. In Campylobacter, 2nd Edition (Irving Nachamkin and Martin J. Blaser, eds). pp. 139-53. Washington D.C.: ASM Press.
2. Anon. (2000). Annual Report on Zoonoses in Denmark 1999 (Brøndsted, T., Hald, T. and Bager, F., eds). Copenhagen, Denmark: The Danish Zoonosis Centre.
3. Harris, N. V., Weiss, N. S. & Nolan, C. M. (1986). The role of poultry and meats in the etiology of Campylobacter jejuni/coli enteritis. American Journal of Public Health 76, 407-11.
4. Deming, M. S., Tauxe, R. V., Blake, P. A., Dixon, S. E., Fowler, B. S. & Jones, T. S. (1987). Campylobacter enteritis at a university: Transmission from eating chicken and from a cat. American Journal of Epidemiology 126, 526-34.
5. Evans, S. J. (1992). Introduction and spread of thermophilic Campylobacter in broiler flocks. Veterinary Record 131, 547-76.
6. Corry, J. E. L. & Atabay H. I. (2001). Poultry as a source of Campylobacter and related organisms. Journal of Applied Microbiology 90, 96S-114S.
7. Genigeorgis, C., Hassuney, M. & Collins, P. (1986). Campylobacter jejuni infection on poultry farms and its effect on poultry meat contamination during slaughter. Journal of Food Protection 49, 895-903.
8. Wedderkopp, A., Rattenborg, E. & Madsen, M. (2000). National surveillance of Campylobacter in broilers at slaughter in Denmark in 1998. Avian Diseases 44, 993-9.
9. Van de Giessen, A. W., Bloemberg, B. P. M., Ritmeester, W. S. & Tilburg, J. J. H. C. (1996). Epidemiological study on risk factors and risk reducing measures for Campylobacter infection in Dutch broiler flocks. Epidemiology and Infection 117, 245-50.
10. Berndtson, E., Emanuelson, U., Engvall, A. & Danielsson-Tham, M. L. (1996). A 1-year epidemiological study of Campylobacter in 18 Swedish chicken farms. Preventive Veterinary Medicine, 26, 167-86.
11. Engvall, A. (1999). Surveillance and Control of Salmonella and Campylobacter in Swedish Commercial Poultry. COST Action 97, Leylystad, The Netherlands, Commission of the European Union.
12. Hald, B., Rattenborg E. & Madsen, M. (2001). Role of batch depletion of broiler houses on the occurence of Campylobacter spp. in chicken flocks. Letters in Applied Microbiology 32, 253-6.
13. Jacobs-Reitsma, W. F., Bolder, N. M. & Mulder, R. W. A. W. (1994). Cecal carriage of Campylobacter and Salmonella in Dutch broiler flocks at slaughter: A one-year study. Poultry Science 73, 1260-6.
14. Oyofo, B. A. & Rollins, D. M. (1993). Efficacy of filter types for detecting Campylobacter jejuni and Campylobacter coli in environmental water samples by polymerase chain reaction. Applied and Environmental Microbiology 59, 4090-5.
15. Kirk, R. & Rowe, M. T. 1994. A PCR assay for the detection of Campylobacter jejuni and Campylobacter coli in water. Letter of Applied Microbiology 19, 301-3.
16. Giesendorf, B. A. J., Quint, W. G. V., Henkens, M. H. C., Stegeman, H., Huf, F. A. & Niester, M. H. C. (1992). Rapid and sensitive detection of Campylobacter spp. in chicken products by the polymerase chain reaction. Applied and Environmental Microbiology 11, 508-10.
17. Wegmuller, B., Luthy, J. & Candrian, U. (1993). Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products. Applied and Environmental Microbiology 59, 2161-5.
18. Hazeleger, W., Arkesteijn, C., Toorop-Bouma, A. & Beumer, R. (1994). Detection of the coccoid form of Campylobacter jejuni in chicken products: The use of the polymerase chain reaction. International Journal of Food Microbiology 24, 273-81.
19. Uyttendaele, M., Schukkink, R., Vangemen, B. & Debevere, J. (1995). Detection of Campylobacter jejuni added to foods by using a combined selective enrichment and nucleic acid sequence-based amplification (NASBA). Applied and Environmental Microbiology 61, 1341-7.
20. Itoh, R., Saitoh, S. & Yatsuyangi, J. (1995). Specific detection of Campylobacter jejuni by means of polymerase chain reaction in chicken litter. Journal of Veterinary Medicine Science 57, 125-7.
21. Bang, D. D., Pedersen, K. & Madsen, M. (2001). Development of a PCR assay suitable for Campylobacter spp. mass screening programs in broiler production. Journal of Rapid Methods and Automation in Microbiology 9, 97-113.
22. Kapperud, G., Vardund, T., Skjerve, E., Hoenes, E. & Michaelsen, T. E. (1993). Detection of pathogenic Yersinia enterocolitica in foods and water by immunomagnetic separation, nested polymerase chain reaction, and colorimetric detection of amplified DNA. Applied and Environmental Microbiology 59, 2938-44.
23. Anon. (1990). Campylobacter jejuni/coli Detection in Food. Method No. 119. 2nd Edn. Esbo, Finland: Nordic Committee on Food Analysis.
24. Bang, D. D., Scheutz, F., Ahrens, P., Pedersen, K., Blom, J. & Madsen, M. (2001). Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers. Journal of Medical Microbiology 50, 1087-94.
25. Machiels, B. M., Ruers, T., Lindhout, M., Hardy, K., Hlavaty, T., Bang, D. D., Somers, V. A., Baeten, C., Von Meyenfeldt, M. & Thunnisen, F. B. (2000). New protocol for DNA extraction of stool. Biotechniques 28, 286-90.
26. Linton, D., Owen, R. J. & Stanley, J. (1996). Rapid identification by PCR of the genus Campylobacter and of five species enteropathogenic for man and animals. Research in Microbiology 147, 707-18.
27. Linton, D., Lawson, A. J., Owen, R. J. & Stanley, J. (1997). PCR detection, identification to species levels and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic sampies. Journal of Clinical Microbiology 35, 2568-72.
28. Hani, E. K. & Chan, V. L. (1995). Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (hippuricase) gene in Escherichia coli. Journal of Bacteriology 177 2396-402.
29. Slater, E. R. & Owen, R. J. (1997). Restriction fragment length polymorphism analysis shows that the hippuricase gene of Campylobacter jejuni is highly conserved. Letters in Applied Microbiology 25, 274-8.
30. Shane, S. M. (2000). Campylobacter infection of commercial poultry. Scientific and Technical Review International Office of Epizooties 19, 376-95.
31. Oijeberg-Bengtsson (1994). NVI/WHO 1994. The Swedish Salmonella program. In: Proceedings of an International Course on Salmonella Control in Animal Production and Products August 1993 (Oijeberg-Bengtsson, ed.). National Veterinary Institute, Malmo, Sweden. pp. 21-7.
32. Humphrey, T. J., Mason, M. & Martin, K. (1995). The isolation of Campylobacter jejuni from contaminated surfaces and its survival in diluents. International Journal of Food Microbiology 26, 295-303.
33. Korhonen, L. K. & Martikainen, P. J. (1990). Comparison of some enrichment broths and growth media for the isolation of thermophilic Campylobacters from surface water samples. Journal of Applied Bacteriology 68, 593-9.
34. Scotter, S. L., Humphrey, T. J. & Henley, A. (1993). Methods for detection of thermotolerant campylobacters in foods: Results of an inter-laboratory study. Journal of Applied Bacteriology 74, 155-63.
35. Mason, M. J., Humphrey, T. J. & Martin, K. W. (1999). Isolation of sublethally injured campylobacters from poultry and water sources. British Journal of Biomedical Science 56, 2-5.
36. Mckay A. M. (1992). Viable but non-culturable form of potential pathogenic bacteria in water. Letters of Applied Microbiology 14, 129-35.
37. Medenma, G. J., Schets F. M., Van de Giessen A. W. & Havelaar A. (1992). Lack of colonization of one-day-old chicks by viable, non-culturable Campylobacter jejuni. Journal of Applied Microbiology 72, 512-16.
38. Jones D. M., Sutcliff, E. M. & Curry, A. (1991). Recovery of viable but non-culturable Campylobacter jejuni. Journal of General Microbiology 137, 2477-82.
39. Saha, S. K., Saha, S., and Sanyal, S. C. (1991). Recovery of injured Campylobacter jejuni cells after animal passage. Applied and Environmental Microbiology 57, 3388-9.
40. Stern, N. J., Jones, D. M., Wesley I. V. & Rollins D. M. (1994). Colonization of chicks by non-culturable Camylobacter spp. Letters in Applied Microbiology 18, 333-6.
41. Beumer, R. R., De Vries, J. & Rombouts F. M. (1992). Campylobacter jejuni non-culturable coccoid cells. International Journal of Food Microbiology 15, 153-63.
42. Van de Giessen, A. W., Heuvelman, C. J., Abee, A. & Hazeleger, W. C. (1996). Experimental studies on the infectivity of nonculturable forms of Campylobacter spp. in chicks and mice. Epidemiology and Infection 117, 463-70.
43. Holler, C., Witthuhn, D. & Janzen-Blunck, B. (1998). Effect of low temperatures on growth, structure and metabolism of Campylobacter coli SP10. Applied and Environmental Microbiology 64, 581-7.
44. Hazeleger, W. C., Wouters, J. A., Rombouts, F. M. & Abee, T. (1998). Physiological activity of Campylobacter jejuni far below the minimal growth temperature. Applied and Environmental Microbiology 64, 3917-22.
45. Hoop, R. & Ehrsam, H. (1987). Ein beitrag zur epidemiologie von Campylobacter jejuni und Campylobacter coli in der huhnermast. Schweizer Archiv fur Tierheilkunde 129, 193-203.
46. Annan-Prah, A. & Janc, M. (1988). The mode of spread of Campylobacter jejuni/coli to broiler flocks. Journal of Veterinary Medicine 35, 11-18.