Expression, purification and characterization of rat procarboxypeptidase B in Pichia pastoris

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 23, Issue 1, 2007, Pages 61-66

  Wang, De Jie ^a,b   Miao, Lin ^b   Chen, Hong ^c   Li, Yan Ying ^b   Chen, Hui Peng ^b   Fang, Hong Qing ^b  

^a JIANGXI SCIENCE AND TECHNOLOGY NORMAL UNIVERSITY   (China)

^b BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^c NORTHWEST A F UNIVERSITY   (China)

Author keywords

Carboxypeptidase B; Expression optimization; Pichia pastoris; Protein purification

Indexed keywords

BOVINAE; PICHIA PASTORIS; RATTUS; SUS;

CARBOXYPEPTIDASE B; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; CATALYSIS; CATTLE; CHEMISTRY; ENZYME SPECIFICITY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; MASS SPECTROMETRY; METABOLISM; MOLECULAR WEIGHT; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; SEQUENCE ANALYSIS; SPRAGUE DAWLEY RAT; SWINE;

ANIMALS; CARBOXYPEPTIDASE B; CATALYSIS; CATTLE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; KINETICS; MASS SPECTROMETRY; MOLECULAR WEIGHT; PICHIA; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SUBSTRATE SPECIFICITY; SWINE;

EID: 68149099044     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Aviles FX, Vendrell J. Carboxypeptidase B. In: Barrett A J, Rrawlings N D, Woessner J F. Handbook of Proteolytic Enzymes. San Diego: Academic Press, 1998, pp. 1333-1335.
2. Pedro BP, Sonia SM, Coll M, et al. Human Procarboxypeptidase B: Three-dimensional Structure and Implications for Thrombin-activatable Fibrinolysis Inhibitor (TAFI). Journal of Molecular Biology, 2002, 321(3): 537-547.
3. Clauser E, Gardell SJ, Craik CS, et al. Structural characterization of the rat carboxypeptidase A1 and B genes. Comparative analysis of the rat carboxypeptidase gene family. Journal of Biological Chemistry, 1988, 263(33): 17837-17845.
4. Skidgel RA, Erdos EG. Cellurar carboxypeptidases. Immunological Reviews, 1998, 161(1): 129-141.
5. Saez J, Martinez J, Trigo C, et al. A comparative study of the activation peptide of carboxypeptidase B and trypsinogen as early predictors of the severity of acute pancreatitis. Pancreas, 2004, 29(1): 9-14.
6. Chen JQ, Zhang HT, Hu MH, et al. Production of human insulin in an E. coil system with Me-Lys-human proinsulin as the expressed precursor. Applied Biochemistry and Biotechnology, 1995, 55(1): 5-9.
7. Eaton DL, Malloy BE, Tsai SP, et al. Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasm. Journal of Biological Chemistry, 1991, 266(32): 21833-21838.
8. Ventura S, Villegas V, Sterner J, et al. Mapping the pro-region of carboxypeptidase B by protein engineering. Journal of Biological Chemistry, 1999, 274(28): 19925-19933.
9. Hartman J, Fulga N, Mendelovitch S, et al. Production of Enzymatically Active Recombinant Carboxypeptidase B. USA: 5948668, 1999-09-07.
10. Li SX, Zhang YJ, Gong Y, et al. Cloning and Expression of a New Rat Procarboxypeptidase B Gene in E. coli and Purification of Recombination Carboxypeptidase B. Protein and Peptide Letters, 2003, 10(6): 581-590.
11. Folk JE, Piez KA, Gladner JA, et al. Carboxypeptidase B. Journal of Biological Chemistry, 1960, 235(8): 2272-2277.
12. Cregg MJ, Cereghino JL, Shi JY, et al. Recombinant Protein Expression in Pichia pastoris. Molcular Biotechnology, 2000, 16(2): 23-52.
13. Ding YF, Liu Y, Dai CB. Advances in Pichia pastoris expression system. Chinese Bulletin of Life Sciences, 2003, 15(1): 26-31.
14. Guarna MM, Cote HC, Amandoron EA, et al. Engineering factor X fusions for expression in Pichia pastoris. Annals of the New York Academy of Sciences, 1996, 1(12): 397-400.
15. Woo JH, Liu YY, Neville DM, et al. Gene optimization is necessary to express a bivalent anti-human anti-T cell immunotoxin in Pichia pastoris. Protein Expression and Purification, 2002, 25(2): 270-282.
16. Marshak DR, Kadonaga RR, Burgess RR, et al. Strategies for Protein Purification and Characterization: A Laboratory Course Manual. Clod Spring Harbor Laboratory Press, 1996, pp. 1-4.