Mapping the pro-region of carboxypeptidase B by protein engineering: Cloning, overexpression, and mutagenesis of the porcine proenzyme

Journal of Biological Chemistry

Volumn 274, Issue 28, 1999, Pages 19925-19933

  Ventura, Salvador ^a   Villegas, Virtudes ^a   Sterner, Jane ^b   Larson, Jeffrey ^b   Vendrell, Josep ^a   Hershberger, Charles L ^b   Avilés, Francesc X ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; CARBOXYPEPTIDASE A; CARBOXYPEPTIDASE B; COMPLEMENTARY DNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME INHIBITION; GENE EXPRESSION; GENE MAPPING; GENE MUTATION; GENE SEQUENCE; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DEGRADATION;

EID: 0033538529     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.28.19925     Document Type: Article

References (37)

1. Neurath, H. (1986) J. Cell. Biochem. 32, 35-49
2. Auld, D. S., and Vallee, B. L. (1987) New Compr. Biochem. 16, 201-256
3. Avilés, F. X., Vendrell, J., Guasch, A., Coll, M., and Huber, R. (1993) Eur. J. Biochem. 211, 391-399
4. Gardell, S. J., Craik, C. S., Clauser, E., Goldsmith, E., Stewart, C-B., Graf, M., and Rutter, W. J. (1988) J. Biol. Chem. 263, 17929-17936
5. Pascual, R., Burgos, F. J., Salvà, M., Soriano, F., Méndez, E., and Avilés, F. X. (1990) Eur. J. Biochem. 179, 609-616
6. Catasús, LI., Vendrell, J., Avilés, F. X., Carreira, S., Puigserver, A., and Billeter, M. (1995) J. Biol. Chem. 270, 6651-6657
7. Reverter, D., García-Sáez I., Catasús, L., Vendrell, J., Coll, M., and Avilés, F. X. (1997) FEBS Lett. 420, 7-10
8. Khan, A. R., and James, M. N. (1998) Protein Sci. 4, 815-836
9. Aloy, P., Catasús, L., Villegas, V., Reverter, D., Vendrell. J., and Avilés, F. X. (1998) Biol. Chem. Hoppe-Seyler 379, 149-155
10. Coll, M., Guasch, A., Avilés, F. X., and Huber, R. (1991) EMBO J. 9, 1-9
11. Guasch, A., Coll, M., Avilés, F. X., and Huber, R. (1992) J. Mol. Biol. 224, 141-157
12. García-Sáez, I., Reverter, D., Vendrell, J., Avilés, F. X., and Coll, M. (1997) EMBO J. 16, 6906-6913
13. Vendrell, J., Billeter, M., Wider, G., Avilés, F. X., and Wüthrich, K. (1991) EMBO J. 10, 11-15
14. Burgos, F. J., Salvà, M., Villegas, V., Soriano, F., Méndez, E., and Avilés, F. X. (1991) Biochemistry 30, 4092-4099
15. Vendrell, J., Cuchillo, C. M., and Avilés, F. X. (1990) J. Biol. Chem. 265, 6949-6953
16. Villegas, V., Vendrell, J., and Avilés, F. X. (1995) Protein Sci. 4, 1792-1900
17. Lacko, A. G., and Neurath, H. (1970) Biochemistry 9, 4680-4690
18. Reeck, G. R., and Neurath, H. (1972) Biochemistry 11, 3947-3955
19. Puissant C., and Houdebine, L, M. (1990) Biotechniques 8, 148-149
20. Frohman, M. A., Dush, M. K., and Martin, G. R. (1998) Proc. Natl. Acad. Sci. U. S. A. 85, 8998-9002
21. Ho, N. S., Hunt, H. D. Horton, R. M. Pullen, J. K., and Pease, L. R. (1989) Gene 77, 51-59
22. Schägger, H., and Von Jagow, G. (1987) Anal. Biochem. 166, 368-379
23. Vendrell, J., and Avilés, F. X. (1986) J. Chromatogr. 358, 401-413
24. Muñoz, V., and Serrano, L. (1995) J. Mol. Biol. 245, 275-296
25. Villegas, V. (1995) Detailed Characterization of the Activation Process of Procarboxypeptidase B through the Use of Inhibitors: Design and Construction of Mutants of the Activation Segment. Ph.D. thesis, Universitat Autènoma de Barcelona
26. Phillips, M. A., and Rutter, W. J. (1996) Biochemistry 35, 6771-6776
27. Laethem, R. M., Blumenkopf, T. A., Cory, M., Elwell, L., Moxham, C. P., Ray, P. H., Walton, L. M., and Smith, G. K., (1996) Arch. Biochem. Biophys. 332, 8-18
28. Reverter, D., Ventura, S., Villegas, V., Vendrell, J., and Avilés, F. X. (1998) J. Biol. Chem. 273, 3535-3541
29. Duffaud, G. D., March, P. E., and Inouye, M. (1997) Methods Enzymol. 153, 492-507
30. Silen, J. L., and Agard, D. A. (1989) Nature 341, 362-364
31. Shinde, U. P., and Inouye, M., (1995) J. Mol. Biol. 247, 390-395
32. Winther, J. R., and Sørensen, P. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 9330-9334
33. Villegas, V., Azuaga, A., Catasús, L., Reverter, D., Mateo, P. L., Avilés, F. X., and Serrano, L. (1995) Biochemistry 34, 15105-15110
34. Villegas, V., Martínez, J. C., Avilés, F. X., and Serrano, L. (1998) J. Mol. Biol. 283, 1027-1036
35. Serra, M. A. (1995) Study of the Peptide Hydrolysis Kinetics of Carboxypeptidase A. Ph.D. thesis, Universitat Autònoma de Barcelona
36. Bajzar, L., Morser, J., and Nesheim, M. E. (1996) J. Biol. Chem. 271, 16603-16608
37. Sakharov D. V., Plow E. F., and Rjjken D. C. (1997) J. Biol. Chem. 272, 14477-14482