Application of a double-reporter-guided mutant selection method to improve clavulanic acid production in Streptomyces clavuligerus

Metabolic Engineering

Volumn 11, Issue 4-5, 2009, Pages 310-318

  Xiang, Si Hai ^a   Li, Jia ^a   Yin, Hua ^a   Zheng, Jian Ting ^a   Yang, Xu ^a   Wang, Hai Bin ^b   Luo, Jia Li ^b   Bai, Hua ^b   Yang, Ke Qian ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b Zhejiang Hisun Pharmaceutical Co Ltd   (China)

Author keywords

Clavulanic acid; Metabolic engineering; Reporter; Strain improvement

Indexed keywords

ALTERNATIVE METHODS; CLAVULANIC ACID; FALSE POSITIVE RATES; GENETIC DIVERSITY; INVERSE METABOLIC ENGINEERING; KANAMYCINS; METABOLIC ENGINEERING; OVER-EXPRESSION; REPORTER; SELECTION METHODS; STRAIN IMPROVEMENT; STREPTOMYCES CLAVULIGERUS; TRANSCRIPTIONAL ACTIVATORS;

BIOCHEMISTRY; CARBOXYLIC ACIDS; METABOLISM; TARGETS;

ACIDS;

CLAVULANIC ACID;

ARTICLE; GENETIC SELECTION; GENETIC VARIABILITY; METABOLIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; REPORTER GUIDED MUTANT SELECTION METHOD; STREPTOMYCES CLAVULIGERUS;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CLAVULANIC ACID; DNA, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENES, REPORTER; GENETIC ENGINEERING; MUTATION; PLASMIDS; STREPTOMYCES; TRANSFORMATION, BACTERIAL;

STREPTOMYCES CLAVULIGERUS;

EID: 68049118544     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2009.06.003     Document Type: Article

References (31)

1. Alper H., and Stephanopoulos G. Global transcription machinery engineering: a new approach for improving cellular phenotype. Metab. Eng. 9 (2007) 258-267
2. Alper H., and Stephanopoulos G. Uncovering the gene knockout landscape for improved lycopene production in E. coli. Appl. Microbiol. Biotechnol. 78 (2008) 801-810
3. Askenazi M., Driggers E.M., Holtzman D.A., Norman T.C., Iverson S., Zimmer D.P., Boers M.E., Blomquist P.R., Martinez E.J., Monreal A.W., Feibelman T.P., Mayorga M.E., Maxon M.E., Sykes K., Tobin J.V., Cordero E., Salama S.R., Trueheart J., Royer J.C., and Madden K.T. Integrating transcriptional and metabolite profiles to direct the engineering of lovastatin-producing fungal strains. Nat. Biotechnol. 21 (2003) 150-156
4. Bailey J.E., Sburlati A., Hatzimanikatis V., Lee K., Renner W.A., and Tsai P.S. Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes. Biotechnol. Bioeng. 52 (1996) 109-121
5. Bignell D.R., Tahlan K., Colvin K.R., Jensen S.E., and Leskiw B.K. Expression of ccaR, encoding the positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus, is dependent on bldG. Antimicrob. Agents Chemother. 49 (2005) 1529-1541
6. Borth N., Mattanovich D., Kunert R., and Katinger H. Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line. Biotechnol. Prog. 21 (2005) 106-111
7. Bro C., and Nielsen J. Impact of 'ome' analyses on inverse metabolic engineering. Metab. Eng. 6 (2004) 204-211
8. Clayton T.M., and Bibb M.J. Streptomyces promoter-probe plasmids that utilise the xylE gene of Pseudomonas putida. Nucleic Acids Res. 18 (1990) 1077
9. Fussenegger M., Schlatter S., Datwyler D., Mazur X., and Bailey J.E. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat. Biotechnol. 16 (1998) 468-472
10. Hirai M.Y., Sugiyama K., Sawada Y., Tohge T., Obayashi T., Suzuki A., Araki R., Sakurai N., Suzuki H., Aoki K., Goda H., Nishizawa O.I., Shibata D., and Saito K. Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis. Proc. Natl. Acad. Sci. USA 104 (2007) 6478-6483
11. Hung T.V., Malla S., Park B.C., Liou K., Lee H.C., and Sohng J.K. Enhancement of clavulanic acid by replicative and integrative expression of ccaR and cas2 in Streptomyces clavuligerus NRRL3585. J. Microbiol. Biotechnol. 17 (2007) 1538-1545
12. Ikeda M., Ohnishi J., Hayashi M., and Mitsuhashi S. A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient l-lysine production. J. Ind. Microbiol. Biotechnol. 33 (2006) 610-615
13. Ingram C., Brawner M., Youngman P., and Westpheling J. xylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolite-controlled promoter. J. Bacteriol. 171 (1989) 6617-6624
14. Kieser T., Bibb M.J., Buttner M.J., Chater K.F., and Hopwood D.A. Practical Streptomyces Genetics (2000), John Innes Foundation, Norwich, England
15. Li R., and Townsend C.A. Rational strain improvement for enhanced clavulanic acid production by genetic engineering of the glycolytic pathway in Streptomyces clavuligerus. Metab. Eng. 8 (2006) 240-252
16. Liu G., Tian Y., Yang H., and Tan H. A pathway-specific transcriptional regulatory gene for nikkomycin biosynthesis in Streptomyces ansochromogenes that also influences colony development. Mol. Microbiol. 55 (2005) 1855-1866
17. Ochi K. From microbial differentiation to ribosome engineering. Biosci. Biotechnol. Biochem. 71 (2007) 1373-1386
18. Olano C., Lombo F., Mendez C., and Salas J.A. Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering. Metab. Eng. 10 (2008) 281-292
19. Paradkar A.S., Aidoo K.A., and Jensen S.E. A pathway-specific transcriptional activator regulates late steps of clavulanic acid biosynthesis in Streptomyces clavuligerus. Mol. Microbiol. 27 (1998) 831-843
20. Paradkar A.S., Mosher R.H., Anders C., Griffin A., Griffin J., Hughes C., Greaves P., Barton B., and Jensen S.E. Applications of gene replacement technology to Streptomyces clavuligerus strain development for clavulanic acid production. Appl. Environ. Microbiol. 67 (2001) 2292-2297
21. Rud I., Solem C., Jensen P.R., Axelsson L., and Naterstad K. Co-factor engineering in lactobacilli: effects of uncoupled ATPase activity on metabolic fluxes in Lactobacillus (L.) plantarum and L. sakei. Metab. Eng. 10 (2008) 207-215
22. Perez-Llarena F.J., Liras P., Rodriguez-Garcia A., and Martin J.F. A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Streptomyces clavuligerus: amplification results in overproduction of both beta-lactam compounds. J. Bacteriol. 179 (1997) 2053-2059
23. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
24. Santamarta I., Rodriguez-Garcia A., Perez-Redondo R., Martin J.F., and Liras P. CcaR is an autoregulatory protein that binds to the ccaR and cefD-cmcI promoters of the cephamycin C-clavulanic acid cluster in Streptomyces clavuligerus. J. Bacteriol. 184 (2002) 3106-3113
25. Schmitt-John T., and Engels J.W. Promoter constructions for efficient secretion expression in Streptomyces lividans. Appl. Microbiol. Biotechnol. 36 (1992) 493-498
26. Shaffer C. Next-generation sequencing outpaces expectations. Nat. Biotechnol. 25 (2007) 149
27. Shendure J., and Ji H. Next-generation DNA sequencing. Nat. Biotechnol. 26 (2008) 1135-1145
28. Stephanopoulos G., Aristidou A.A., and Nielsen J. Metabolic Engineering: Principles and Methodologies (1998), Academic Press, San Diego
29. Tahlan K., Anders C., and Jensen S.E. The paralogous pairs of genes involved in clavulanic acid and clavam metabolite biosynthesis are differently regulated in Streptomyces clavuligerus. J. Bacteriol. 186 (2004) 6286-6297
30. Wang L., Tahlan K., Kaziuk T.L., Alexander D.C., and Jensen S.E. Transcriptional and translational analysis of the ccaR gene from Streptomyces clavuligerus. Microbiology 150 (2004) 4137-4145
31. Yuan L.Z., Rouviere P.E., Larossa R.A., and Suh W. Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli. Metab. Eng. 8 (2006) 79-90