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Volumn 131, Issue 7, 2009, Pages 2657-2662

Origin of high fidelity in target-sequence recognition by PNA Ce(IV)/EDTA combinations as site-selective DNA cutters

Author keywords

[No Author keywords available]

Indexed keywords

AMINES; CERIUM; CERIUM COMPOUNDS; DNA; EXCITONS; GELATION; GENES; MELTING POINT; METAL ANALYSIS; TARGETS; TEMPERATURE MEASUREMENT;

EID: 67849121882     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja808290e     Document Type: Article
Times cited : (44)

References (39)
  • 11
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    • For this purpose, naturally occurring nucleases were combined with DNA-binding molecules. Alternatively, restriction enzyme sites in substrate DNA, except for the target site, were protected by methylation, and then this substrate was treated with the enzyme. See:(a). Strobel, S. A.; Dervan, P. E. Nature 1991, 350, 172-174.
    • For this purpose, naturally occurring nucleases were combined with DNA-binding molecules. Alternatively, restriction enzyme sites in substrate DNA, except for the target site, were protected by methylation, and then this substrate was treated with the enzyme. See:(a). Strobel, S. A.; Dervan, P. E. Nature 1991, 350, 172-174.
  • 20
    • 34249847680 scopus 로고    scopus 로고
    • A monophosphate group was attached to the N-terminus of each of these two pcPNAs to improve the scission efficiency. See: Yamamoto, Y, Mori, M, Aiba, Y, Tomita, T, Chen, W, Zhou, J.-M, Uehara, A, Ren, Y, Kitamura, Y, Komiyama, M. Nucleic Acids Res. 2007, 35, e53
    • A monophosphate group was attached to the N-terminus of each of these two pcPNAs to improve the scission efficiency. See: Yamamoto, Y.; Mori, M.; Aiba, Y.; Tomita, T.; Chen, W.; Zhou, J.-M.; Uehara, A.; Ren, Y.; Kitamura, Y.; Komiyama, M. Nucleic Acids Res. 2007, 35, e53.
  • 27
    • 0032738421 scopus 로고    scopus 로고
    • Induction of local structural changes in double-stranded DNA by PNA invasion has been clearly evidenced. See: a, Lohse, J, Dahl, O, Nielsen, P. E. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 11804-11808
    • Induction of local structural changes in double-stranded DNA by PNA invasion has been clearly evidenced. See: (a). Lohse, J.; Dahl, O.; Nielsen, P. E. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 11804-11808.
  • 30
    • 37849040469 scopus 로고    scopus 로고
    • Rapireddy, S.; He, G.; Roy, S.; Armitage, B. A.; Ly, D. H. J. Am. Chem. Soc. 2007, 129, 1559615600 Furthermore, enzymatic reactions were controlled by PNA invasion. See:
    • Rapireddy, S.; He, G.; Roy, S.; Armitage, B. A.; Ly, D. H. J. Am. Chem. Soc. 2007, 129, 1559615600) Furthermore, enzymatic reactions were controlled by PNA invasion. See:
  • 37
    • 84925567853 scopus 로고    scopus 로고
    • The scission bands in lanes 3-5, if present, were at least 100-fold weaker than those in lane 2
    • The scission bands in lanes 3-5, if present, were at least 100-fold weaker than those in lane 2.
  • 38
    • 84925567852 scopus 로고    scopus 로고
    • The gel mobilities of the invasion complexes in Figure 6 differed from lane to lane. It is probable that the magnitude of the bending of the substrate DNA induced by the invasion is dependent on the kind of mismatch incorporated at the invasion site.
    • The gel mobilities of the invasion complexes in Figure 6 differed from lane to lane. It is probable that the magnitude of the bending of the substrate DNA induced by the invasion is dependent on the kind of mismatch incorporated at the invasion site.
  • 39
    • 84925561456 scopus 로고    scopus 로고
    • It should be noted that the invasion complex has pseudo-C 2 symmetry when the difference in sequence is neglected see Figure 1
    • 2 symmetry when the difference in sequence is neglected (see Figure 1).


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