PDC1, a corn defensin peptide expressed in Escherichia coli and Pichia pastoris inhibits growth of Fusarium graminearum

Peptides

Volumn 30, Issue 9, 2009, Pages 1593-1599

  Kant, Pragya ^a   Liu, Wen Zhe ^a   Pauls, K Peter ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Antifungal activity; Defensin; Fusarium graminearum; Protein expression; Zea mays

Indexed keywords

PLANT DEFENSIN CORN 1 PROTEIN; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ANTIFUNGAL ACTIVITY; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; FUSARIUM GRAMINEARUM; GROWTH INHIBITION; INFRARED SPECTROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL PROLIFERATION; DEFENSINS; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; FUSARIUM; GERMINATION; HYPHAE; MOLECULAR SEQUENCE DATA; PHYLOGENY; PICHIA; PLANT DISEASES; PLANT PROTEINS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; ZEA MAYS;

ESCHERICHIA COLI; EUKARYOTA; GIBBERELLA ZEAE; HORDEUM; PICHIA PASTORIS; PROKARYOTA; ZEA MAYS;

EID: 67849084834     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.peptides.2009.05.024     Document Type: Article

References (31)

1. Bloch Jr. C., and Richardson M. A new family of small (5 kDa) protein inhibitors of insect α-amylases from seeds or sorghum (Sorghum bicolor (L) Moench) have sequence homologies with wheat γ-purothionins. FEBS Lett 279 (1991) 101-104
2. Broekaert W.F., Cammue B.P.A., Bolle De M.F.C., Thevissen K., Samblanx De G.W., and Osborn R.W. Antimicrobial peptides from plants. Crit Rev Plant Sci 16 (1997) 297-323
3. Broekaert W.F., Terras F.R.G., Cammue B.P.A., and Vanderleyden J. An automated quantitative assay for fungal growth inhibition. FEMS Microbiol Lett 69 (1990) 55-60
4. Byler D.M., and Susi H. Examination of the secondary structure of proteins by deconvolved FTIR spectra. Biopolymers 25 (1986) 469-487
5. Castro M.S., Fontes W., Morhy L., and Bloch Jr. C. Complete amino acid sequences of two gamma-thionins from maize (Zea mays L.) seeds. Protein Pept Lett 3 (1996) 267-274
6. Carson M., Johnson D.H., McDonald H., Brouillette C., and DeLucas L.J. His-tag impact on structure. Biol Crystallogragh D63 (2007) 295-301
7. Carvalho A.O., and Gomes V.M. Plant defensins- Prospects for the biological functions and biotechnological properties. Peptides 30 (2009) 1007-1020
8. Cipakova I., Gasperik J., and Hostinova E. Expression and purification of human antimicrobial peptide, dermicidin in Escherichia coli. Protein Express Purif 45 (2006) 269-274
9. Colilla F.J., Rocher A., and Mendez E. Gamma-Purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm. FEBS Lett 270 (1990) 191-194
10. Cregg J.M., Vedvick T.S., and Raschke W.C. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology 11 (1993) 905-910
11. Gao A.G., Hakimi S.M., Mittanck C.A., Wu Y., Woerner B.M., Stark D.M., et al. Fungal pathogen protection in potato by expression of a plant defensin peptide. Nat Biotechnol 12 (2000) 1307-1310
12. Heck G.R., and David Ho T.H. Gibberellin-repressible gene expression in the barley aleurone layer. Plant Mol Biol 30 (1996) 611-623
13. Hoisington D.A., Khairallah M.M., and Gonzales-de-Leon D. Laboratory protocols. CIMMYT Applied Molecular Genetics Laboratory (1994), CIMMYT, Hisfoa, Mexico: DF
14. Huang L., Wang J., Zhong Z., Peng L., Chen H., Xu Z., et al. Production of bioactive human β-defensin-3 in Escherichia coli by soluble fusion expression. Biotechnol Lett 28 (2006) 627-632
15. Kramer G., Kudlicki W., McCaethy D., Tsalkova T., Simmons D., and Hardesty B. N-terminal and C-terminal modifications affect folding, release from the ribosomes and stability of in-vitro synthesized proteins. Inter J Biochem Cell Biol 31 (1999) 231-241
16. Kushmerick C., Castro M.D., Cruz J.S., Bloch Jr. C., and Beirao P.S.L. Functional and structural features of gamma-zeathionins, a new class of sodium channel blockers. FEBS Letters 440 (1998) 302-306
17. Mendez E., Moreno A., Colilla F.J., Pelaez F., Limas G.G., Mendez R., et al. Primary structure and inhibition of protein synthesis in eukaryotic cell-free system of a novel thionin, gamma-hordothionin, from barley endosperm. Eur J Biochem 194 (1990) 533-539
18. Miller J.D. Epidemiology of Fusarium ear disease of cereals. In: Miller J.D., and Trenholm H.L. (Eds). Mycotoxins in grain: compounds other that aflatoxin (1994), Eagan Press, St. Paul, Minn. 302-303
19. Munkvold G.P. Cultural and genetic approaches to managing mycotoxins in maize. Annu Rev Phytopathol 41 (2003) 99-116
20. Petra E., Klaus A., and Holger B. ESTs reveal a multigene family for plant defensins in Arabidopsis thaliana. FEBS Lett 400 (1997) 168-172
21. Punja K.Z. Genetic engineering of plants to enhance resistance to fungal pathogens: a review of progress and future prospects. Can J Plant Pathol 23 (2001) 216-235
22. Spelbrink R.G., Dilmac N., Allen A., Smith T.J., Shah D.M., and Hockerman G.H. Differential antifungal and calcium channel-blocking activity among structurally related plant defensins. Plant Physiol 135 (2004) 2055-2067
23. Stack R.W.A. Comparison of the inoculum potential of ascospores and conidia of Gibberella zeae. Can J Plant Pathol 11 (1989) 137-142
24. Sutton J.C. Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum. Can J Plant Pathol 4 (1982) 195-209
25. Terras F.R., Eggermont K., Kovaleva V., Raikhel N.V., Osborn R.W., Kester A., et al. Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7 (1995) 573-588
26. Thevissen K., Warnecke D.C., François I.E.J.A., Leipelt M., Heinz E., Ott C., et al. Defensins from insects and plants interact with fungal glucosylceramides. J Biol Chem 279 (2004) 3900-3905
27. Thomma B.P.H.J., and Broekaert W.F. Tissue-specific expression of plant defensin genes PDF2.1 and PDF2.2 in Arabidopsis thaliana. Plant Physiol Biochem 36 (1998) 533-537
28. Thomma B.P.H.J., Cammue B.P.A., and Thevissen K. Plant defensins. Planta 216 (2002) 193-202
29. Turkov M., Rashi Z., Noam D., Gordon R.B., Khalifa M., Stankiewicz M., et al. In-vitro folding and functional analysis of an anti-insect selective scorpion depressant nurotoxin produced in Escherichia coli. Protein Express Purif 10 (1997) 123-131
30. Vigier B., Reid L.M., Dwyer L.M., Stewart D.W., Sinha R.C., Arnason J.T., et al. Maize resistance to Gibberella ear rot: symptoms, Deoxynivalenol, and yield. Can J Plant Pathology 23 (2001) 99-105
31. Yuan Y., Gao B., and Zhu S. Functional expression of a Drosophila antifungal peptide in Escherichia coli. Protein Expr Purif 52 (2007) 457-462