Comparative quantification of Campylobacter jejuni from environmental samples using traditional and molecular biological techniques

Canadian Journal of Microbiology

Volumn 55, Issue 6, 2009, Pages 633-641

  Rothrock Jr , Michael J ^a   Cook, Kimberly L ^a   Bolster, Carl H ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Campylobacter jejuni; Escherichia coli; Quantitative real time PCR; Selective plate counts

Indexed keywords

CAMPYLOBACTER JEJUNI; CELL CONCENTRATIONS; COMPARATIVE RESEARCH; E. COLI; ENVIRONMENTAL HEALTH; ENVIRONMENTAL SAMPLE; IN-CELL; INDICATOR ORGANISMS; LEACHATE SAMPLES; LIMIT OF DETECTION; MOLECULAR-BIOLOGICAL TECHNIQUES; POTENTIAL RISKS; QUANTITATIVE REAL-TIME PCR; SELECTIVE PLATE COUNTS; WATER SAMPLES;

ASSAYS; CELL MEMBRANES; CONCENTRATION (PROCESS); HEALTH RISKS; LEACHING;

ESCHERICHIA COLI;

BACTERIUM; COMPARATIVE STUDY; DETECTION METHOD; LEACHATE; MOLECULAR ANALYSIS; PATHOGEN; POLYMERASE CHAIN REACTION; REAL TIME;

ACCURACY; ARTICLE; BACTERIUM DETECTION; CAMPYLOBACTER JEJUNI; CONTROLLED STUDY; ESCHERICHIA COLI; INDICATOR ORGANISM; INTERMETHOD COMPARISON; LEACHING; NONHUMAN; PLATE COUNT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WATER SAMPLING;

CAMPYLOBACTER JEJUNI; COLONY COUNT, MICROBIAL; ENVIRONMENTAL MICROBIOLOGY; ESCHERICHIA COLI; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALIA; CAMPYLOBACTER JEJUNI; ESCHERICHIA COLI;

EID: 67749135432     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W09-006     Document Type: Article

References (43)

1. Baggi, F., Demarta, A., and Peduzzi, R. 2001. Persistence of viral pathogens and bacteriophages during sewage treatment: lack of correlation with indicator bacteria. Res. Microbiol. 152: 743-751. doi:10.1016/S0923-2508(01) 01255-4. PMID:11686388.
2. Bolster, C.H., Mills, A.L., Hornberger, G.M., and Herman, J.S. 2001. Effect of surface coatings, grain size, and ionic strength on the maximum attainable coverage of bacteria on sand surfaces. J. Contam. Hydrol. 50: 287-305. doi:10.1016/S0169-7722(01)00106-1. PMID:11523329.
3. Bolster, C.H., Walker, S.L., and Cook, K.L. 2006. Comparison of Escherichia coli and Campylobacter jejuni transport in saturated porous media. J. Environ. Qual. 35: 1018-1025. doi:10.2134/ jeq2005.0224. PMID:16738386.
4. Buswell, C.M., Herlihy, Y.M., Lawrence, L.M., McGuiggan, J.T.M., Marsh, P.D., Keevil, C.W., and Leach, S.A. 1998. Extended survival and persistence of Campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescentantibody and -rRNA staining. Appl. Environ. Microbiol. 64: 733-741. PMID:9464415.
5. Colles, F.M., Jones, K., Harding, R.M., and Maiden, M.C.J. 2003. Genetic diversity of Campylobacter jejuni isolates from farm animals and the farm environment. Appl. Environ. Microbiol. 69: 7409-7413. doi:10.1128/AEM.69.12. 7409-7413.2003. PMID: 14660392.
6. Cook, K.L., and Bolster, C.H. 2007. Survival of Campylobacter jejuni and Escherichia coli in groundwater during prolonged starvation at low temperatures. J. Appl. Microbiol. 103: 573-583. doi:10.1111/j.1365-2672.2006.03285.x. PMID:17714390.
7. Ferguson, M.F., Coote, B.G., Ashbolt, N.J., and Stevenson, I.M. 1996. Relationships between indicators, pathogens, and water quality in an estuarine system. Water Res. 30: 2045-2054. doi:10.1016/0043-1354(96)00079-6. (Pubitemid 27094268)
8. Frahm, E., and Obst, U. 2003. Application of the fluorogenic probe technique (TaqMan PCR) to the detection of Enterococcus spp. and Escherichia coli in water samples. J. Microbiol. Methods, 52: 123-131. doi:10.1016/S0167- 7012(02)00150-1. PMID: 12401234.
9. Fuhrman, J.A., Corneau, D.E., Hagstrom, A., and Chan, A.M. 1988. Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies. Appl. Environ. Microbiol. 54: 1426-1429. PMID:16347652.
10. Hanninen, M.-L., Haaganen, H., Pummi, T., Wermundsen, K., Katila, M.-L., Sarkkinen, H., et al. 2003. Detection and typing of Campylobacter jejuni and Campylobacter coli and analysis of indicator organisms in three waterborne outbreaks in Finland. Appl. Environ. Microbiol. 69: 1391-1396. doi:10.1128/AEM.69. 3.1391-1396.2003. PMID:12620821.
11. Harwood, V.J., Levine, A.D., Scott, T.M., Chivukula, V., Lukasik, J., Farrah, S.R., and Rose, J.B. 2005. Validity of the indicator organism paradigm for pathogen reduction in reclaimed water and public health protection. Appl. Environ. Microbiol. 71: 3163-3170. doi:10.1128/AEM.71.6.3163-3170.2005. PMID: 15933017.
12. Hazeleger, W.C., Wouters, J.A., Rombouts, F.M., and Abee, T. 1998. Physiological activity and Campylobacter jejuni far below minimal growth temperature. Appl. Environ. Microbiol. 64: 3917-3922. PMID:9758819.
13. Hutchison, M.L., Walters, L.D., Avery, S.M., Synge, B.A., and Moore, A. 2004. Levels of zoonotic agents in British livestock manures. Lett. Appl. Microbiol. 39: 207-214. doi:10.1111/j. 1472-765X.2004.01564.x. PMID:15242463.
14. Inglis, G.D., and Kalischuk, L.D. 2004. Direct quantification of Campylobacter jejuni and Campylobacter lenienae in feces of cattle by real-time quantitative PCR. Appl. Environ. Microbiol. 70: 2296-2306. doi:10.1128/AEM.70.4. 2296-2306.2004. PMID: 15066825.
15. Jenkins, M.B., Endale, D.M., and Fisher, D.S. 2008. Most probable number methodology for quantifying dilute concentrations and fluxes of Salmonella in surface waters. J. Appl. Microbiol. 104: 1562-1568. doi:10.1111/j.1365-2672. 2007.03677.x. PMID: 18179540.
16. Jones, K. 2001. Campylobacters in water, sewage and the environment. J. Appl. Microbiol. 90: 68S-79S. doi:10.1046/j.1365-2672.2001.01355.x.
17. Jones, D.M., Sutcliffe, E.M., and Curry, A. 1991. Recovery of viable but nonculturable Campylobacter jejuni. J. Gen. Microbiol. 137: 2477-2482. PMID:1770362.
18. Korhonen, L., and Martikainen, P.J. 1991. Survival of Escherichia coli and Campylobacter jejuni in untreated and filtered lake water. J. Appl. Microbiol. 71: 379-382. doi:10.1111/j.1365-2672.1991.tb03804.x.
19. Lemarchand, K., and Lebaron, P. 2003. Occurence of Salmonella spp. and Cryptosporidium spp. in a French coastal watershed: relationship with fecal indicators. FEMS Microbiol. Ecol. 218: 203-209. doi:10.1111/j.1574-6968.2003. tb11519.x.
20. Mead, P.S., Slustsker, L., Dietz, V., McCaig, F., Bresee, J.S., Shapiro, C., et al. 1999. Food related illness and death in the United States. Emerg. Infect. Dis. 5: 607-625. PMID:10511517.
21. Medema, G.J., Schets, F.M., van de Giessen, A.W., and Havelaar, A.H. 1992. Lack of colonization of 1 day old chicks by viable non-culturable Campylobacter jejuni. J. Appl. Bacteriol. 72: 512-516. PMID:1644708.
22. Murinda, S.E., Nguyen, L.T., Nam, H.M., Almeida, R.A., Headrick, S.J., and Oliver, S.P. 2004. Detection or sorbitol-negative and sorbitol-positive shigo toxin-producing Escherichia coli, Listeria monocytogenes, Campylobacter jejuni, and Salmonella spp. in dairy farm environmental samples. Foodborne Pathog. Dis. 1: 97-104. doi:10.1089/153531404323143611. PMID: 15992268.
23. Murphy, C., Carroll, C., and Jordan, K.N. 2006. Environmental survival mechanisms of the foodborne pathogen Campylobacter jejuni. J. Appl. Microbiol. 100: 623-632. doi:10.1111/j.1365-2672. 2006.02903.x. PMID:16553716.
24. Nocker, A., and Camper, A.K. 2006. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl. Environ. Microbiol. 72: 1997-2004. doi:10.1128/AEM.72.3.1997-2004.2006. PMID:16517648.
25. Nogva, H.K., Bergh, A., Holck, A., and Rudi, K. 2000. Application of the 5′-nuclease PCR assay in evaluation and development of methods for quantitative detection of Campylobacter jejuni. Appl. Environ. Microbiol. 66: 4029-4036. doi:10.1128/AEM.66. 9.4029-4036.2000. PMID:10966425. (Pubitemid 30691888)
26. Park, S.F. 2002. The physiology of Campylobacter species and its relevance to their role as foodborne pathogens. Int. J. Food Microbiol. 74: 177-188. doi:10.1016/S0168-1605(01)00678-X. PMID:11981968.
27. Pearson, A.D., Greenwood, M., Healing, T.D., Rollins, D., Shamat, M., Donaldson, J., and Colwell, R.R. 1993. Colonization of broiler chickens by waterborne Campylobacter jejuni. Appl. Environ. Microbiol. 59: 987-996. PMID:8476300.
28. Peters, J., Wolf, A., and Wolf, N. 2003. Ammonium nitrogen. In Recommended methods of manure analysis. Edited by J. Peters. University of Wisconsin Extension, Madison, Wisconsin. pp. 25-29.
29. Rinttila, T., Kassinen, A., Malinen, E., Krogius, L., and Palva, A. 2004. Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J. Appl. Microbiol. 97: 1166-1177. doi:10.1111/j.1365-2672.2004.02409.x. PMID: 15546407.
30. Rollins, D.M., and Colwell, R.R. 1986. Viable but nonculturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment. Appl. Environ. Microbiol. 52: 531-538. PMID:3767358.
31. Sails, A.D., Fox, A.J., Bolton, F.J., Wareing, D.R.A., and Greenway, D.L.A. 2003. A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture. Appl. Environ. Microbiol. 69: 1383-1390. doi:10.1128/AEM.69.3.1383-1390. 2003. PMID:12620820.
32. Schaffter, N., and Parriaux, A. 2002. Pathogenic-bacterial water contamination in mountainous catchments. Water Res. 36: 131-139. doi:10.1016/S0043-1354(01)00242-1. PMID:11766788. (Pubitemid 33035049)
33. Stanley, K., and Jones, K. 2003. Cattle and sheep farms as reservoirs for Campylobacter. J. Appl. Microbiol. 94: 104S-113S. doi:10.1046/j.1365-2672.94. s1.12.x. PMID:12675942.
34. Stern, N.J., Fedorka-Cray, P.J., Bailey, J.S., Cox, N.A., Craven, S.E., Hiett, K.L., et al. 2001. Distribution of Campylobacter spp. in selected US poultry production and processing operations. J. Food Prot. 64: 1705-1710. PMID:11726147.
35. van de Giessen, A.W., Heuvelman, C.J., Abee, T., and Hazeleger, W.C. 1996. Experimental studies on the infectivity of non-culturable forms of Campylobacter spp. in chicks and mice. Epidemiol. Infect. 117: 463-470. PMID:8972670.
36. Waage, A.S., Vardund, T., Lund, V., and Kapperud, G. 1999. Detection of small numbers of Campylobacter jejuni and Campylobacter coli cells in environmental water, sewage, and food samples by a seminested PCR assay. Appl. Environ. Microbiol. 65: 1636-1643. PMID:10103261.
37. Wallace, J.S., Stanley, K.N., and Jones, K. 1993. Seasonal effects of natural sunlight on the survival of Campylobacter jejuni, Escherichia coli, Salmonella enteritidis, and Klebsiella pneumonia. Acta Gastroenterol. Belg. 56: 33-35.
38. Walter, P.J., Chalk, S., and Kingston, H.M. 1997. Overview of microwave- assisted sample preparation. In Microwave-enhanced chemistry: fundamentals, sample preparation, and applications. Edited by H.M. Kinston and S.J. Haswell. American Chemical Society, Washington, D.C. pp. 55-222.
39. Watson, M., Wolf, A., and Wolf, N. 2003. Total nitrogen. In Recommended methods of manure analysis. Edited by J. Peters. University of Wisconsin Extension. Madison, Wisconsin. pp. 18-24.
40. Weinbauer, M.G., Fritz, I., Wenderoth, D.F., and Hofle, M.G. 2002. Simultaneous extraction from bacterioplankton of total RNA and DNA suitable for quantitative structure and function analyses. Appl. Environ. Microbiol. 68: 1082-1087. doi:10. 1128/AEM.68.3.1082-1087.2002. PMID:11872453.
41. Wolffs, P.F.G., Glencross, K., Thibaudeau, R., and Griffiths, M.W. 2006. Direct quantitation and detection of Salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR. Appl. Environ. Microbiol. 72: 3896-3900. doi:10.1128/AEM.02112-05. PMID:16751494.
42. Yang, C., Jiang, Y., Huang, K., Zhu, C., and Yin, Y. 2003. Application of real-time PCR for quantitative detection of Campylobacter jejuni in poultry, milk, and environmental water. FEMS Immunol. Med. Microbiol. 38: 265-271. doi:10.1016/S0928-8244(03)00168-8. PMID:14522462.
43. Ziprin, R.L., and Harvey, R.B. 2004. Inability of cecal microflora to promote reversion of viable nonculturable Campylobacter jejuni. Avian Dis. 48: 647-650. doi:10.1637/7153-010504R1. PMID:15529989.