Detection of opportunistic DNA viral infections by multiplex PCR among HIV infected individuals receiving care at a tertiary care hospital in South India

Indian Journal of Medical Microbiology

Volumn 27, Issue 3, 2009, Pages 210-216

  Sachithanandham, J ^a   Ramamurthy, M ^a   Kannangai, R ^a   Daniel, H D ^a   Abraham, O C ^a   Rupali, P ^a   Pulimood, S A ^a   Abraham, A M ^a   Sridharan, G ^a  

^a CHRISTIAN MEDICAL COLLEGE   (India)

Author keywords

Human immunodeficiency virus; India; Multiplex polymerase chain reaction; Opportunistic viruses

Indexed keywords

VIRUS DNA;

ADENOVIRUS; ADULT; ARTICLE; BK VIRUS; BLOOD SAMPLING; CD4 LYMPHOCYTE COUNT; CONTROLLED STUDY; CYTOMEGALOVIRUS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DISEASE ASSOCIATION; DISEASE COURSE; EARLY DIAGNOSIS; EPSTEIN BARR VIRUS; FEMALE; HERPES SIMPLEX VIRUS 1; HERPES SIMPLEX VIRUS 2; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENT; INDIA; JC VIRUS; MAJOR CLINICAL STUDY; MALE; MORBIDITY; MORTALITY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; OPPORTUNISTIC INFECTION; QUANTITATIVE ANALYSIS; TERTIARY HEALTH CARE; VARICELLA ZOSTER VIRUS; VIRUS DETECTION; VIRUS INFECTION; VIRUS LOAD;

EID: 67749104163     PISSN: 02550857     EISSN: 19983646     Source Type: Journal    
DOI: 10.4103/0255-0857.53202     Document Type: Article

References (26)

1. Xu LP, Zhang HY, Huang XJ, Liu KY, Liu DH, Han W, et al. Hemorrhagic cystitis following hematopoietic stem celltransplantation: Incidence, risk factors and association with CMV reactivation and graft-versus-host disease. Chin Med J 2007;120:1666-71.
2. Musacchio JG, Carvalho Mda G, Morais JC, Silva NH, Scheliga A, Romano S, et al. Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin's disease. Sao Paulo Med J 2006;124:154-7.
3. Hogg RS, Yip B, Chan KJ, Wood E, Craib KJP, O'Shaughnessy MV, et al. Rates of disease progression by baseline CD4 cell count and viral load after initiating triple-drug therapy. JAMA 2001;286:2568-77.
4. De La Blanchardiere A, Rozenberg F, Caumes E, Picard O, Lionnet F, Livartowski J, et al. Neurological complications of varicella-zoster virus infection in adults with human immunodefciency virus infection. Scand J Infect Dis 2000;32:263-9.
5. Schneider U, Ruhnke M, Delecluse HJ, Stein H, Huhn D. Regression of Epstein-Barr virus-associated lymphoproliferative disorders in patients with acquired immunodefciency syndrome during therapy with foscarnet. Ann Hematol 2000;79:214-6.
6. Kagu MB, Nggada HA, Garandawa HI, Askira BH, Durosinml MA. AIDS-associated Kaposi's sarcoma in Northeastern Nigeria. Singapore Med J 2006;47:1069-74.
7. Elfaitouri A, Hammarin AL, Blomberg J. Quantitative real-time PCR assay for detection of human polyomavirus infection. J Virol Methods 2006;135:207-13.
8. Hierholzer JC. Adenoviruses in the immunocompromised host. Clin Microbiol Rev 1992; 5:262-74.
9. Marshall DS, Linfert DR, Draghi A., McCarter YS, Tsongalis GJ. Identifcation of Herpes simplex virus genital infection: Comparison of a Multiplex PCR assay and traditional viral isolation techniques. Mod Pathol 2001;14:152-6.
10. Elnifro EM, Ashshi AM, Cooper RJ, Klapper PE. Multiplex PCR: Optimization and application in diagnostic virology. Clin Microbiol Rev 2000;13:559-70.
11. Stevens SJ, Pronk I, Middeldrop JM. Toward Standardization of Epstein-barr Virus DNA load monitoring: Unfractionated whole blood as preferred clinical specimen. J Clin Microbiol 2001;39:1211-6.
12. Spector SA, Mckinley GF, Lalezari JP, Samo T, Andruczk R, Follansbee S, et al. Oral ganciclovir for the prevention of cytomegalovirus disease in persons with AIDS. N Engl J Med 1996;334:1491-7.
13. Markoulatos P, Georgopoulou A, Siafakas N, Plakokefalos E, Tzanakaki G, Kourea-Kremastinou J. Laboratory diagnosis of common Herpesvirus infections of the central nervous system by a Multiplex PCR assay. J Clin Microbiol 2001;39:4426-32.
14. Echavarria M, Forman M, Ticehurst J, Dumler JS, Charache P. PCR method for detection of Adenovirus in urine of healthy and human immunodefciency virus-infected individuals. J Clin Microbiol 1998;36:3323-6.
15. Biel SS, Held TK, Landt O, Niedrig M, Gelderblom HR, Siegert W, et al. Rapid quantifcation and differentation of human Polyomavirus DNA in undiluted urine from patients after bone marrow Transplantation. J Clin Microbiol 2000;38:3689-95.
16. Palmer S, Wiegand AP, Maldarelli F, Bazmi H, Mican JM, Polis M, et al. New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodefciency virus type -1 RNA in plasma. J Clin Microbiol 2003;41:4531-6.
17. Watzinger F, Suda M, Preuner S, Baumgartinger R, Ebner K, Baskova L, et al. Real time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosupressed pediatric patients. J Clin Microbiol 2004;42:5189-98.
18. Miller CS, Berger JR, Mootoor Y, Avdiushko SA, Zhu H, Kryscio RJ. High prevalence of multiple human Herpesviruses in saliva from human immunodefciency virus-infected persons in the era of highly active Antiretroviral Therapy. J Clin Microbiol 2006;44:2409-15.
19. Rojanawiwat A, Miura T, Thaisri H, Pathipvanich P, Umnajsirisuk S, Koibuchi T, et al. Frequent detection of Epstein-Barr virus and Cytomegalovirus but not JC virus DNA in cerebrospinal fuid samples from human immunodefciency virus infected patients in northern Thailand. J Clin Microbiol 2005;43:3484-6.
20. Gerna G, Baldanti F, Lilleri D, Parea M, Alessandrino E, Pagani A, et al. Human Cytomegalovirus Immediate-Early mRNA Detection by Nucleic Acid Sequence-Based Amplifcation as a New Parameter for Preemptive Therapy in Bone Marrow Transplant Recipients. J Clin Microbiol 2000;38:1845-53.
21. Wada K, Kubota N, Ito Y, Yagasaki H, Kato K, Yoshikawa T, et al. Simultaneous quantitation of Epstein barr virus, cytomegalovirus and human herpesvirus 6 DNA in samples from transplant recipients by multiplex real time PCR assay. J Clin Microbiol 2007;45:1426-32.
22. Panagiotakis SH, Soufa G, Baritaki S, Sourvinos G, Passam A, Zagoreos I, et al. Concurrent CMV and EBV DNAemia is Signifcantly correlated with a delay in the response to HAART in treatment-naive HIV type 1-positive patients. AIDS Res and Hum Retrovir 2007;23:10-8.
23. Edwards J, Constable F, Wiechel T, Salib S. Comparison of the molecular tests - single PCR, nested PCR and quantitative PCR (SYBR®Green and TaqMan®) - for detection of Phaeomoniella chlamydospora during grapevine nursery propagation. Phytopathol Mediterr 2007;46:58-72.
24. Brantsaeter AB, Holberg-Petersen M, Jeansson S, Goplen AK, Bruun JN. CMV quantitative PCR in the diagnosis of CMV disease in patients with HIV-infection - a retrospective autopsy based study. BMC Infect Dis 2007;7:127-34.
25. Jebbink J, Bai X, Rogers BB, Dawson DB, Scheuermann RH, Domiati-Saad R. Development of real time PCR assays for the quantitative detection of Epstein-Barr virus and cytomegalovirus, comparison of TaqMan probes, and molecular beacons. J Mol Diagn 2003;5:15-20.
26. Murdoch DM, Venter WDF, Rie AV, Feldman C. Immune reconstitution infammatory syndrome (IRIS): review of common infectious manifestations and treatment options. AIDS Res Ther 2007;4:9.