Group III PLA2 from the scorpion, Mesobuthus tamulus: Cloning and recombinant expression in E. coli

Electronic Journal of Biotechnology

Volumn 12, Issue 3, 2009, Pages

  Hariprasad, Gururao ^a   Saravanan, Kolandaivelu ^a   Singh, Sundararajan Baskar ^a   Das, Utpal ^a   Sharma, Sujata ^a   Kaur, Punit ^a   Singh, Tej Pal ^a   Srinivasan, Alagiri ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Group III phospholipase A2; Mesobuthus tamulus; Recombinant expression

Indexed keywords

C-TERMINAL EXTENSIONS; CALCIUM BINDING MOTIFS; CATALYTIC RESIDUE; CLONING AND EXPRESSION; DENATURING CONDITIONS; E. COLI; FREE FATTY ACID; FUNCTIONAL CONFORMATIONS; GROUP III; GROUP III PHOSPHOLIPASE A2; HEMOLYTIC ASSAYS; MESOBUTHUS TAMULUS; METAL-AFFINITY CHROMATOGRAPHY; NUCLEOTIDE SEQUENCES; OPEN READING FRAME; OPTIMUM PH; PHOSPHOLIPASES; PRIMARY STRUCTURES; RECOMBINANT EXPRESSION; REFOLDING; SECRETORY PHOSPHOLIPASE; SERIAL DILUTIONS; SIGNAL PEPTIDE; TOXIC PROTEINS;

AFFINITY CHROMATOGRAPHY; AMINES; AMINO ACIDS; CALCIUM; CALCIUM ALLOYS; CHROMATOGRAPHIC ANALYSIS; CLONING; ENZYMES; ESCHERICHIA COLI; FATTY ACIDS; METAL REFINING; ORGANIC ACIDS; PHOSPHOLIPIDS;

PLANTS (BOTANY);

AMINO ACID; CALCIUM; CYSTEINE; PHOSPHOLIPASE A2 GROUP III; SIGNAL PEPTIDE;

AFFINITY CHROMATOGRAPHY; ANIMAL TISSUE; ARTICLE; CALCIUM BINDING; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; ENZYME ASSAY; ENZYME CONFORMATION; ENZYME PURIFICATION; ESCHERICHIA COLI; HEMOLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN STRUCTURE; SCORPION;

ESCHERICHIA COLI; MESOBUTHUS TAMULUS; SCORPIONES;

EID: 67749088655     PISSN: None     EISSN: 07173458     Source Type: Journal    
DOI: 10.2225/vol12-issue3-fulltext-7     Document Type: Article

References (22)

1. CHOMCZYNSKI, Piotr and SACCHI, Nicoletta. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, April 1987, vol.162, no.1, p. 156-159.
2. CONDE, Renaud; ZAMUDIO, Fernando Z.; BECERRIL, Baltazar and POSSANI, Lourival D. Phospholipin, a novel heterodimeric phospholipase A2 from Pandinus imperator scorpion venom. FEBS Letters, November 1999, vol.460, no.3, p. 447-450.
3. 2 causes apoptosis in rat primary cortical neuronal cultures. Brain Research, October 2003, vol.988, no.1-2, p. 20-28.
4. DUCKERT, Peter; BRUNAK, Soren and BLOM, Nikolaj. Prediction of proprotein convertase cleavage sites. Protein Engineering, Design and Selection, January 2004, vol.17, no.1, p. 107-112.
5. 2 variant in Heloderma suspectum (Gila monster) venom. European Journal of Biochemistry, December 1989, vol.186, no.1-2, p. 23-33.
6. 2 from Heterometrus fulvipes (Indian black scorpion). DNA Sequence, June 2007, vol.18, no.3, p. 242-246.
7. 2: its usefulness in laboratory diagnostics. Critical Reviews in Clinical Laboratory Sciences, April 1999, vol.36, no.2, p. 65-163.
8. 2 from honeybee venom glands. The deduced aminoacid sequence reveals homology to the corresponding vertebrate enzymes. European Journal of Biochemistry, 1989, vol.184, no.1, p. 249-254.
9. MANZUR, María J.; MUÑOS, Rosana V.; LUCERO, Adrián A.; AYUB, Maximiliano J.; ALVAREZ, Sergio E. and CIUFFO, Gladys M. Production of recombinant enzymes of wide use for research. Electronic Journal of Biotechnology, April 2006, vol.9, no.3, p. 291-296.
10. 2s (groups III and XII). Journal of Biological Chemistry, March 2003, vol.278, no.12, p. 10657-10667.
11. 2. Journal of Biological Chemistry, April 2005, vol.280, no.26, p. 24987-24998.
12. PETRUNKEVITCH, Alexander. Arthropoda 2: Chelicerata with sections on Pycnogonida and Palaeoisopus In: MOORE, R.C. ed. Treatise on invertebrate paleontology-Part P. Geological Society of America and University of Kansas press, Lawrence, 1955, 181 p.
13. RYU, Yoonseok; OH, Youn Sang; YOON, Jaeseung; CHO, Wonhwa and BAEK, Kwanghee. Molecular characterization of a gene encoding the Drosophila melanogaster phospholipase A2. Biochemica et Biophysica Acta-Gene Structure and Expresion, July 2003, vol.1628, no.1, p. 206-210. (Pubitemid 36976821)
14. rd ed. New York, Cold Spring Harbor Laboratory Press, 2001. 999 p. ISBN 0-87969-577-3.
15. SCOTT, David L.; OTWINOWSKI, Zbyszek; GELB, Micheal H. and SIGLER, Paul B. Crystal structure of beevenom phospholipase A2 in a complex with a transitionstate analogue. Science, December 1990, vol.250, no.4987, p. 1563-1566. (Pubitemid 120034367)
16. SHILOAH, J.; KLIBANSKY, C.; DE VRIES, A. and BERGER, A. Phospholipase B activity of a purified phospholipase A from Vipera palestinae venom. Journal of Lipid Research, May 1973, vol.14, no.3, p. 267-278.
17. 2 enzymes: classification and characterization. Biochemica et Biophysica Acta-Molecular and Cell Biology of Lipids, October 2000, vol.1488, no.1-2, p. 1-19.
18. 2 and their pathophysiological significance in inflammatory diseases. Current Molecular Medicine, December 2001, vol.1, no.6, p. 739-754.
19. 2 from the venom of the scorpion Anuroctonus phaiodactylus. European Journal of Biochemistry, April 2004, vol.271, no.8, p. 1453-1464.
20. 2 with homology to the group III bee venom enzyme. Journal of Biological Chemistry, March 2000, vol.275, no.11, p. 7492-7496.
21. WU, Pei-Fung and CHANG, Long-Sen. Genetic organization of A chain and B chain of β-bungarotoxin from Taiwan banded krait (Bungarus multicinctus): A chain genes and B chain genes do not share a common origin. European Journal of Biochemistry, August 2000, vol.267, no.15, p. 4668-4675.
22. ZAMUDIO, Fernando Z.; CONDE, Renaud; AREVALO, Carolina; BECERRIL, Baltazar; MARTIN, Brian M.; VALDIVIA, Hector H. and POSSANI, Lourival D. The mechanism of inhibition of ryanodine receptor channels by imperatoxin I, a heterodimeric protein from the scorpion Pandinus imperator. Journal of Biological Chemistry, May 1997, vol.272, no.18, p. 11886-11894.