Cloning, sequence analysis and homology modeling of a novel phospholipase A2 from Heterometrus fulvipes (Indian black scorpion)

DNA Sequence - Journal of DNA Sequencing and Mapping

Volumn 18, Issue 3, 2007, Pages 242-246

  Hariprasad, ^a   Singh, Baskar ^a   Das, Utpal ^a   Ethayathulla, Abdul S ^a   Kaur, Punit ^a   Singh, Tej P ^a   Srinivasan, Alagiri ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Heterometrus fulvipes; Indian black scorpion; PhospholipaseA2

Indexed keywords

ASPARTIC ACID; HISTIDINE; PHOSPHOLIPASE A2; SCORPION VENOM;

AMINO ACID SEQUENCE; ARTICLE; CALCIUM BINDING; DISULFIDE BOND; DNA SEQUENCE; ENZYME ACTIVE SITE; HUMAN VERSUS ANIMAL COMPARISON; MODEL; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN MOTIF; SCORPION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHOSPHOLIPASES A; SCORPIONS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

APOIDEA; HETEROMETRUS FULVIPES; MESOBUTHUS TAMULUS;

EID: 34247374305     PISSN: 10425179     EISSN: 10292365     Source Type: Journal    
DOI: 10.1080/10425170701243294     Document Type: Article

References (18)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215:403-410.
2. Annand RR, Kontoyianni M, Penzotti JE, Dudler T, Lybrand TP, Gelb MH. 1996. Active site of bee venom phospholipase A2: The role of histidine-34, aspartate-64 and tyrosine-87. Biochemistry 35:4591-4601.
3. Chomczynski P, Sacchi N. 1987. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
4. DeLano WL. 2002. The PyMOL molecular graphics system. San Carlos, CA, USA: DeLano scientific.
5. Kini RM, Evans HJ. 1989. A model to explain the pharmacological effects of snake venom phospholipase A2. Toxicon 27:613-635.
6. Kondo K, Toda H, Narita K, Lee CY. 1982. Amino acid sequence of beta2-bungarotoxin from Bungarus multicinctus venom. The amino acid substitutions in the B chains. J Biochem 91:1519-1530.
7. Kuchler K, Gmachl M, Sippl MJ, Kreil G. 1989. Analysis of the cDNA for phospholipase A2 from honeybee venom glands. The deduced amino acid sequence reveals homology to the corresponding vertebrate enzymes. Eur J Biochem 184:249-254.
8. Murakami M, Masuda S, Shimbara S, Ishikawa Y, Ishii I, Kudo I. 2005. Cellular distribution, post-translational modification, and tumorigenic potential of human group III secreted phospholipase A(2). J Biol Chem 280:24987-24998.
9. Ramanaiah M, Parthasarthy M, Venkaiah B. 1990. Purification and properties of phospholipase A2 from the venom of scorpion, (Heterometrus fulvipes). Biochem Int 20:931-940.
10. Ryu Y, Oh Y, Yoon J, Cho W, Baek K. 2003. Molecular characterization of a gene encoding the Drosophila melanogaster phospholipase A2. Biochim Biophys Acta 25(1628):206-210.
11. Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467.
12. Scott DL, Otwinowski Z, Gelb MH, Sigler PB. 1990. Crystal structure of bee-venom phospholipase A2 in a complex with a transition-state analogue. Science 250:1563-1566.
13. Six DA, Dennis EA. 2000. The expanding superfamily of phospholipase A(2) enzymes: Classification and characterization. Biochim Biophys Acta 1488:1-19.
14. Sosa BP, Alagon AC, Martin BM, Possani LD. 1986. Biochemical characterization of the phospholipase A2 purified from the venom of the Mexican beaded lizard (Heloderma horridum horridum wiegmann). Biochemistry 25:2927-2933.
15. Swede T, Kopp J, Guex N, Peitsch MC. 2003. SWISS-MODEL: An automated protein homology-modeling server. Nucleic Acids Res 31:3381-3385.
16. Valdez-Cruz NA, Batista CV, Possani LD. 2004. Phaiodactylipin, a glycosylated heterodimeric phospholipase A from the venom of the scorpion Anuroctonus phaiodactylus. Eur J Biochem 271:1453-1464.
17. Valentin E, Ghomashchi F, Gelb MH, Lazdunski M, Lambeau. 2000. Novel human secreted phospholipase A(2) with homology to the group III bee venom enzyme. J Biol Chem 275:7492-7496.
18. Zamudio FZ, Conde R, Arevalo C, Becerril B, Martin BM, Valdivia HH, Possani LD. 1997. The mechanism of inhibition of ryanodine receptor channels by imperatoxin I, a heterodimeric protein from the scorpion Pandinus imperator. J Biol Chem 272:11886-11894.