Production of recombinant enzymes of wide use for research

Electronic Journal of Biotechnology

Volumn 9, Issue 3, 2006, Pages 291-296

  Manzur, María J ^a   Muñoz, Rosana V ^a   Lucero, Adrián A ^a   Ayub, Maximiliano Juri ^a   Alvarez, Sergio E ^a   Ciuffo, Gladys M ^a  

^a UNIVERSIDAD NACIONAL DE SAN LUIS   (Argentina)

Author keywords

Bioactivity; Protein expression; Purification; Recombinant enzymes

Indexed keywords

ENZYMES; MOLECULAR STRUCTURE; PROTEINS; PURIFICATION;

BIOACTIVITY; PROTEIN EXPRESSION; RECOMBINANT ENZYMES; STANDARD PROTOCOLS;

BIOTECHNOLOGY;

RECOMBINANT ENZYME; RNA DIRECTED DNA POLYMERASE; TAQ POLYMERASE;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; DRUG RESEARCH; ENZYME ACTIVITY; ENZYME MODIFICATION; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; MOLONEY LEUKEMIA ONCOVIRUS; NONHUMAN; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE MEASUREMENT;

MURINAE;

EID: 33745833005     PISSN: 07173458     EISSN: 07173458     Source Type: Journal    
DOI: 10.2225/vol9-issue3-16     Document Type: Article

References (17)

1. ARCE, M.E.; SANCHEZ, S.; SELTZER, A. and CIUFFO, G.M. Autoradiographic localization of angiotensin II receptors in developing rat cerebellum and brainstem. Regulatory Peptides, 2001, vol. 99, no. 1, p. 53-60.
2. th ed. Wiley, USA, 1999. 1104 p. ISBN 0-471-32938-X.
3. nd ed. 1996. 432 p. ISBN 0-471-11837-0.
4. BOWDEN, G.A.; PAREDES, A.M. and GEORGIOU, G. Structure and morphology of protein inclusion bodies in E. coli. Bio/Technology, 1991, vol. 9, p. 725-730.
5. CHAFFOTTE, A.F.; GUILLOU, Y. and GOLDBERG, M.E. Inclusion bodies of the thermophillic endoglucanase D from Clostridium thermocellium are made of native enzyme that resist 8M urea. European Journal of Biochemistry, 1992, vol. 205, p. 369-373.
6. CIUFFO, G.M.; JOHREN, O., EGIDY, G.; HEEMSKERK, F.M.J. and SAAVEDRA, J.M. Heterogeneity of rat Ang II AT2 receptors. In: RAIZADA, M.; PHILLIP, I. and SUMMERS, C. eds. Recent Advances in Angiotensin Receptors. Plenum Press, 1996, p. 189-197.
7. DIEFFENBACH, C.W. and DVEKSLER, G.S. PCR Primer. A laboratory manual. Cold Spring Harbour: Laboratory Press, 1995. ISBN 0-87969-447-5.
8. ENGELKE, D.R.; KRISKOS, A.; BRUCK, M.E. and GINSBURG, D. Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Analytical Biochemistry, 1990, vol. 191, no. 2, p. 396-400.
9. LAWYER, F.C.; STOFFEL, S.; SAIKI, R.K.; MYAMBO, K.; DRUMMOND, R. and GELFAND, D.H. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. Journal of Biological Chemistry, 1989, vol. 264, no. 11, p. 6427-6437.
10. NICKENIG, G.; SACHINIDIS, A.; MICHAELSEN, F.; BOHM, M.; SEEWALD, S.; VETTER, H. Upregulation of vascular Angiotensin II receptor gene expression by lowdensity lipoprotein in vascular smooth muscle cells. Circulation, 1997, vol. 95, no. 2, p. 473-478.
11. OTTINO, P. Rapid purification of high activity Taq DNA polymerase expressed in transformed E. coli cells. Transactions of the Zimbabwe Scientific Association, 1998, vol. 72, p. 23-26.
12. PUNGITORE, C.R.; AYUB, M.J.; BORKOWSKI, E.J.; TONN C.E. and CIUFFO G.M. Inhibition of Taq DNA polymerase by catalpol. Cellular and Molecular Biology, 2004, vol. 50, no. 6, p. 767-772.
13. PRICE, N.C. and STEVENS, L. The purification of enzymes. In: Fundamentals of enzymology: The cell and molecular biology of catalytic proteins. Oxford University Press, 1999, p. 15-46.
14. PLUTHERO, F.G. Rapid purification of high-activity Taq DNA polymerase. Nucleic Acids Research, 1993, vol. 21, no. 20, p. 4850-4851.
15. SUN, D.; JESSEN, S.; LIU, C.; LIU, X; NAJMUDIN, S. and GEORGIADIS, M.M. Cloning, expression, and purification of a catalytic fragment of Moloney murine leukaemia virus reverse transcriptase: Crystallization of nucleic acid complexes. Protein Science, 1998, vol. 7, no. 7, p. 1575-1582.
16. TAUBE, R.; LOYA, S.; VIDAN, O.A.; PERACH, M. and HIZI, A. Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization. Biochemical Journal, 1998, vol. 329, no. 3, p. 579-587.
17. THATCHER, D.R. and HITCHCOCK, A. Protein folding in biotechnology. In: PAIN, R.H. ed. Mechanism of Protein Folding. Oxford University Press, 1994. p. 229-261.