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Volumn 14, Issue 12, 2008, Pages 2698-2704

Stabilization of RNA during laser capture microdissection by performing experiments under argon atmosphere or using ethanol as a solvent in staining solutions

Author keywords

Argon; Ethanolic staining; Laser capture microdissection; RNA stabilization

Indexed keywords

ALCOHOL; RIBONUCLEASE;

EID: 57049178485     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.1261708     Document Type: Article
Times cited : (69)

References (22)
  • 1
    • 0036315025 scopus 로고    scopus 로고
    • Isolating vessels from the mouse brain for gene expression analysis using laser capture microdissection
    • Ball, H.J., McParland, B., Driussi, C., and Hunt, N.H. 2002. Isolating vessels from the mouse brain for gene expression analysis using laser capture microdissection. Brain Res. Brain Res. Protoc. 9: 206-213.
    • (2002) Brain Res. Brain Res. Protoc , vol.9 , pp. 206-213
    • Ball, H.J.1    McParland, B.2    Driussi, C.3    Hunt, N.H.4
  • 4
    • 32644435694 scopus 로고    scopus 로고
    • Degradation of RNA in bacteria: Comparison of mRNA and stable RNA
    • Deutscher, M.P. 2006. Degradation of RNA in bacteria: Comparison of mRNA and stable RNA. Nucleic Acids Res. 34: 659-666.
    • (2006) Nucleic Acids Res , vol.34 , pp. 659-666
    • Deutscher, M.P.1
  • 7
    • 0032926691 scopus 로고    scopus 로고
    • Immuno-LCM: Laser capture microdissection of immunostained frozen sections for mRNA analysis
    • Fend, F., Emmert-Buck, M.R., Chuaqui, R., Cole, K., Lee, J., Liotta, L.A., and Raffeld, M. 1999. Immuno-LCM: Laser capture microdissection of immunostained frozen sections for mRNA analysis. Am. J. Pathol. 154: 61-66.
    • (1999) Am. J. Pathol , vol.154 , pp. 61-66
    • Fend, F.1    Emmert-Buck, M.R.2    Chuaqui, R.3    Cole, K.4    Lee, J.5    Liotta, L.A.6    Raffeld, M.7
  • 8
    • 33644975004 scopus 로고    scopus 로고
    • RNA integrity and the effect on the real-time qRT-PCR performance
    • Fleige, S. and Pfaffl, M.W. 2006. RNA integrity and the effect on the real-time qRT-PCR performance. Mol. Aspects Med. 27: 126-139.
    • (2006) Mol. Aspects Med , vol.27 , pp. 126-139
    • Fleige, S.1    Pfaffl, M.W.2
  • 9
    • 40649129032 scopus 로고    scopus 로고
    • Frost, A.R., Eltoum, I.E., and Siegal, G.P. 2001. Laser capture microdissection. In Current protocols in molecular biology, Chap. 25, Sec. A, pp. 25A.1.1-25A.1.24. Wiley, New York.
    • Frost, A.R., Eltoum, I.E., and Siegal, G.P. 2001. Laser capture microdissection. In Current protocols in molecular biology, Chap. 25, Sec. A, pp. 25A.1.1-25A.1.24. Wiley, New York.
  • 10
    • 0033022067 scopus 로고    scopus 로고
    • Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue
    • Goldsworthy, S.M., Stockton, P.S., Trempus, C.S., Foley, J.F., and Maronpot, R.R. 1999. Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol. Carcinog. 25: 86-91.
    • (1999) Mol. Carcinog , vol.25 , pp. 86-91
    • Goldsworthy, S.M.1    Stockton, P.S.2    Trempus, C.S.3    Foley, J.F.4    Maronpot, R.R.5
  • 11
    • 0029937192 scopus 로고    scopus 로고
    • Microdissection RT-PCR analysis of gene expression in pathologically defined frozen tissue sections
    • Hiller, T., Snell, L., and Watson, P.H. 1996. Microdissection RT-PCR analysis of gene expression in pathologically defined frozen tissue sections. Biotechniques 21: 38-40, 42, 44.
    • (1996) Biotechniques , vol.21
    • Hiller, T.1    Snell, L.2    Watson, P.H.3
  • 12
    • 0036403539 scopus 로고    scopus 로고
    • Optimized tissue processing and staining for laser capture microdissection and nucleic acid retrieval
    • Huang, L.E., Luzzi, V., Ehrig, T., Holtschlag, V., and Watson, M.A. 2002. Optimized tissue processing and staining for laser capture microdissection and nucleic acid retrieval. Methods Enzymol. 356: 49-62.
    • (2002) Methods Enzymol , vol.356 , pp. 49-62
    • Huang, L.E.1    Luzzi, V.2    Ehrig, T.3    Holtschlag, V.4    Watson, M.A.5
  • 13
    • 28644452204 scopus 로고    scopus 로고
    • Toward standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces
    • doi: 10.1093/nar/gni054
    • Imbeaud, S., Graudens, E., Boulanger, V., Barlet, X., Zaborski, P., Eveno, E., Mueller, O., Schroeder, A., and Auffray, C. 2005. Toward standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Res. 33: e56. doi: 10.1093/nar/gni054.
    • (2005) Nucleic Acids Res , vol.33
    • Imbeaud, S.1    Graudens, E.2    Boulanger, V.3    Barlet, X.4    Zaborski, P.5    Eveno, E.6    Mueller, O.7    Schroeder, A.8    Auffray, C.9
  • 15
    • 0344844555 scopus 로고    scopus 로고
    • Liver gene expression profiles of rats treated with clofibric acid: Comparison of whole liver and laser capture microdissected liver
    • Michel, C., Desdouets, C., Sacre-Salem, B., Gautier, J.C., Roberts, R., and Boitier, E. 2003. Liver gene expression profiles of rats treated with clofibric acid: Comparison of whole liver and laser capture microdissected liver. Am. J. Pathol. 163: 2191-2199.
    • (2003) Am. J. Pathol , vol.163 , pp. 2191-2199
    • Michel, C.1    Desdouets, C.2    Sacre-Salem, B.3    Gautier, J.C.4    Roberts, R.5    Boitier, E.6
  • 17
    • 0742289540 scopus 로고    scopus 로고
    • Development of rapid staining protocols for laser-capture microdissection of brain vessels from human and rat coupled to gene expression analyses
    • Mojsilovic-Petrovic, J., Nesic, M., Pen, A., Zhang, W., and Stanimirovic, D. 2004. Development of rapid staining protocols for laser-capture microdissection of brain vessels from human and rat coupled to gene expression analyses. J. Neurosci. Methods 133: 39-48.
    • (2004) J. Neurosci. Methods , vol.133 , pp. 39-48
    • Mojsilovic-Petrovic, J.1    Nesic, M.2    Pen, A.3    Zhang, W.4    Stanimirovic, D.5
  • 21
    • 33746368013 scopus 로고    scopus 로고
    • Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA
    • Wang, H., Owens, J.D., Shih, J.H., Li, M.C., Bonner, R.F., and Mushinski, J.F. 2006. Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. BMC Genomics 7: 97.
    • (2006) BMC Genomics , vol.7 , pp. 97
    • Wang, H.1    Owens, J.D.2    Shih, J.H.3    Li, M.C.4    Bonner, R.F.5    Mushinski, J.F.6
  • 22
    • 33646887537 scopus 로고    scopus 로고
    • Angiostatin decreases cell migration and vascular endothelium growth factor (VEGF) to pigment epithelium derived factor (PEDF) RNA ratio in vitro and in a murine ocular melanoma model
    • Yang, H., Xu, Z., Iuvone, P.M., and Grossniklaus, H.E. 2006. Angiostatin decreases cell migration and vascular endothelium growth factor (VEGF) to pigment epithelium derived factor (PEDF) RNA ratio in vitro and in a murine ocular melanoma model. Mol. Vis. 12: 511-517.
    • (2006) Mol. Vis , vol.12 , pp. 511-517
    • Yang, H.1    Xu, Z.2    Iuvone, P.M.3    Grossniklaus, H.E.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.