A method to determine Young's modulus of soft gels for cell adhesion

Acta Mechanica Sinica/Lixue Xuebao

Volumn 25, Issue 4, 2009, Pages 565-570

  Xiong, Chunyang ^a   Huang, Jianyong ^a   Qin, Lei ^a   Peng, Xiaoling ^a   Fang, Jing ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

Bending measurement; Cell adhesion; Polyacrylamide gel; Young's modulus

Indexed keywords

ACRYLAMIDE; BEAM MODEL; BENDING CURVATURE; BENDING MEASUREMENT; CELLULAR ADHESION; CONTACT PROBLEM; CULTURE MEDIUM; POLYACRYLAMIDE GEL; POLYACRYLAMIDE GELS; SELF ADAPTIVE ALGORITHMS; SOFT GELS; SOFT MATERIAL; TESTING DEVICE; YOUNG'S MODULUS;

ADAPTIVE ALGORITHMS; ADHESION; AMIDES; BENDING (DEFORMATION); CELL ADHESION; CELL CULTURE; CELL MEMBRANES; ELASTICITY; GELATION; GELS; OPTICAL SENSORS;

ELASTIC MODULI;

EID: 67651225136     PISSN: 05677718     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10409-009-0270-6     Document Type: Article

References (19)

1. C.G. Galbraith M.P. Sheetz 1998 Forces on adhesive contacts affect cell function. Curr. Opin. Cell Biol. 10 566-571.
2. V. Vogel M.P. Sheetz 2006 Local force and geometry sensing regulate cell functions. Nat. Rev. Mol. Cell Biol. 7 265-275.
3. T. Yeung P.C. Georges L.A. Flanagan B. Marg M. Ortiz M. Funaki N. Zahir W.Y. Ming V. Weaver P.A. Janmey 2005 Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. Cell Motil. Cytoskelet. 60 24-34.
4. R.J. Pelham Y.L. Wang 1997 Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc. Natl. Acad. Sci. USA. 94 13661-13665.
5. L. Qin J.Y. Huang C.Y. Xiong Y.Y. Zhang J. Fang 2007 Dynamical stress characterization and energy evaluation of single cardiac myocyte actuating on flexible substrate. Biochem. Biophys. Res. Commun. 360 352-356.
6. M. Dembo Y.L. Wang 1999 Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophys. J. 76 2307-2316.
7. S. Munevar Y.L. Wang M. Dembo 2001 Traction force microscopy of migrating normal and H-Ras transformed 3T3 fibroblasts. Biophys. J. 80 1744-1757.
8. N. Wang I.M. Tolic-Norrelykke J.X. Chen S.M. Mijailovich J.P. Butler J.J. Fredberg D. Stamenovic 2002 Cell prestress. I. stiffness and prestress are closely associated in adherent contractile cells. Am. J. Physiol. Cell Physiol. 282 606-616.
9. J. Lee M. Leonard T. Oliver A. Ishihara K. Jacobson 1994 Traction force generated by locomoting keratocytes. J. Cell Biol. 127 1957-1964.
10. J.Y. Huang X.L. Peng L. Qin T. Zhu C.Y. Xiong Y.Y. Zhang J. Fang 2009 Determination of cellular tractions on elastic substrate based on an integral Boussinesq solution. J. Biomech. Eng.-Trans. ASME. 131 061009 10.1115/1.3118767.
11. Y. Li C. Wen H. Xie A. Ye Y. Yin 2009 Mechanical property analysis of stored red blood cell using optical tweezers. Colloids Surf. B. 70 169-173.
12. E.K. Dimitriadis F. Horkay J. Maresca B. Kachar R.S. Chadwick 2002 Determination of elastic moduli of thin layers of soft material using the atomic force microscope. Biophys. J. 82 2798 2810.
13. M. Zhang Y.P. Zheng A.F.T. Mak 1997 Estimating the effective Young's modulus of tissue from indentation tests-nonlinear finite element analysis of effects of friction and large deformation. Med. Eng. Phys. 19 512-517.
14. S. Chen L. Liu T. Wang 2004 Nanoindentation of thin-film-substrate system: determination of film hardness and Young's modulus. Acta Mech. Sin. 20 (4) 383-392.
15. K.C. Wu H. You 2007 Determination of solid material elastic modulus and surface energy based on JKR contact model. Appl. Surf. Sci. 253 8530-8537.
16. Timoshenko S., Gere J.M.: Mechanics of Materials. Van Nostrand Reinhold, New York (1972).
17. T. Boudou J. Ohayon Y. Arntz G. Finet C. Picart P. Tracqui 2006 An extended modeling of the micropipette aspiration experiment for the characterization of the Young's modulus and Poisson's ratio of adherent thin biological samples: Numerical and experimental studies. J. Biomech. 39 1677-1685.
18. P. Tracqui J. Ohayon T. Boudou 2008 Theoretical analysis of the adaptive contractile behaviour of a single cardiomyocyte cultured on elastic substrates with varying stiffness. J. Theor. Biol. 255 92-105.
19. Y. Xiang D.A. LaVan 2007 Analysis of soft cantilevers as force transducers. Appl. Phys. Lett. 90 133901.