Positive-selection vector for direct protein expression

BioTechniques

Volumn 46, Issue 6, 2009, Pages 453-457

  Haag, Andreas F ^a,b   Ostermeier, Christian ^b  

^a UNIVERSITY OF APPLIED SCIENCES WEIHENSTEPHAN TRIESDORF   (Germany)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Direct expression; Direct selection; Positive selection; Protein expression; Seamless cloning

Indexed keywords

AMPICILLIN; BETA LACTAMASE; PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CASSETTE; GENE FUNCTION; GENE INSERTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; STOP CODON; GENE EXPRESSION; GENE VECTOR; GENETIC SELECTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PENICILLIN RESISTANCE;

ESCHERICHIA COLI;

AMPICILLIN RESISTANCE; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PROTEINS; SELECTION (GENETICS);

EID: 67651055943     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113091     Document Type: Article

References (28)

1. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY.
2. Hashimoto, Y., T. Miki, M. Mukae, T. Ueda, and T. Imoto. 1998. Construction of a yeast expression system with positive selection for gene insertion in the absence of a specific phenotype. Gene 207:167-170.
3. Burns, D.M. and I.R. Beacham. 1984. Positive selection vectors: A small plasmid vector useful for the direct selection of Sau3A-generated overlapping DNA fragments. Gene 27:323-325.
4. Ahmed, A. 1984. Plasmid vectors for positive galactose-resistance selection of cloned DNA in Escherichia coli. Gene 28:37-43.
5. Reddy, M. 2004. Positive selection system for identification of recombinants using α-complementation plasmids. BioTechniques 37:948-952. (Pubitemid 39612553)
6. Schneider, S., O. Georgiev, M. Buchert, M.T. Adams, K. Moelling, and C.M. Hovens. 1997. An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts. Gene 197:337-341.
7. Hennecke, H., I. Gunther, and F. Binder. 1982. A novel cloning vector for the direct selection of recombinant DNA in E. coli. Gene 19:231-234.
8. Dean, D. 1981. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15:99-102.
9. Hashimoto-Gotoh, T., A. Kume, and W. Masahashi. 1986. Improved vector, pHSG664, for direct streptomycin-resistance selection: cDNA cloning with G: C-tailing procedure and subcloning of double-digest DNA fragments. Gene 41:125-128.
10. Schumann, W. and Ch. Loegl. 1980. Plasmid vectors derived from phage Mu allow direct selection of transformants containing cloned HindIII and PstI fragments. Mol. Gen. Genet. 179:369-372.
11. Honigman, A., A.B. Oppenheim, B. Hohn, and T. Hohn. 1981. Plasmid vectors for positive selection of DNA inserts controlled by the lambda p(L) promoter, repressor and antitermination function. Gene 13:289-298.
12. Bernard, P., P. Gabarit, E.M. Bahassi, and M. Couturier. 1994. Positive-selection vectors using the F plasmid ccdB killer gene. Gene 148:71-74. (Pubitemid 24327048)
13. Bernard, P. 1995. New ccdB- positive-selection cloning vectors with kanamycin or chloramphenicol selectable markers. Gene 162:159-160.
14. Choi, Y.J., T.T. Wang, and B.H. Lee. 2002. Positive selection vectors. Crit. Rev. Biotechnol. 22:225-244.
15. Huang, W., J. Petrosino, M. Hirsch, P.S. Shenkin, and T. Palzkill. 1996. Amino acid sequence determinants of β-lactamase structure and activity. J. Mol. Biol. 258:688-703.
16. Szybalski, W., S.C. Kim, N. Hasan, and A.J. Podhajska. 1991. Class-IIS restriction enzymes-A review. Gene 100:13-26.
17. Benoit, R.M., R.N. Wilhelm, D. Scherer-Becker, and C. Ostermeier. 2006. An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids. Protein Expr. Purif. 45:66-71.
18. Studier, F.W. 2005. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41:207-234.
19. Studier, F.W. and B.A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
20. Chamberlin, M. and J. Ring. 1973. Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme. J. Biol. Chem. 248:2235-2244.
21. Studier, F.W., A.H. Rosenberg, J.J. Dunn, and J.W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
22. Studier, F.W. 1991. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J. Mol. Biol. 219:37-44.
23. Kohli, B.M. and C. Ostermeier. 2003. A rubredoxin based system for screening of protein expression conditions and on-line monitoring of the purification process. Protein Expr. Purif. 28:362-367.
24. Bernard, P., K.E. Kezdy, L. Van Melderen, J. Steyaert, L. Wyns, M.L. Pato, P.N. Higgins, and M. Couturier. 1993. The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase. J. Mol. Biol. 234:534-541.
25. Shimomura, O., F.H. Johnson, and Y. Saiga. 1962. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J. Cell. Comp. Physiol 59:223-239.
26. Prasher, D.C., V.K. Eckenrode, W.W. Ward, F.G. Prendergast, and M.J. Cormier. 1992. Primary structure of the Aequorea victoria green-f luorescent protein. Gene 111:229-233.
27. Shu, X., N.C. Shaner, C.A. Yarbrough, R.Y. Tsien, and S.J. Remington. 2006. Novel chromophores and buried charges control color in mFruits. Biochemistry 45:9639-9647.
28. Aslanidis, C. and P.J. De Jong. 1990. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18:6069-6074. (Pubitemid 20364668)