Analysis of global DNA methylation levels in human blood using high-performance liquid chromatography/tandem electrospray ionization mass spectrometry

European Journal of Mass Spectrometry

Volumn 15, Issue 4, 2009, Pages 555-561

  Ma, Huimin ^a,b   Zhang, Wenbing ^a   Hu, Junjie ^a,b   Yu, Zhiqiang ^a   Chen, Yong ^a,b   Luo, Qiong ^c   Shi, Xiaoliu ^d   Zhang, Yingying ^e   Song, Renfang ^a   Zhou, Zhen ^a   Sheng, Guoying ^a   Fu, Jiamo ^a  

^a GUANGZHOU INSTITUTE OF GEOCHEMISTRY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c Licheng Hospital   (China)

^d CENTRAL SOUTH UNIVERSITY   (China)

^e JACOBS UNIVERSITY BREMEN   (Germany)

Author keywords

Aging; Electrospray ionization mass spectrometry; Global DNA methylation; Human blood

Indexed keywords

DNA;

ADOLESCENT; ADULT; AGE; AGED; ARTICLE; BLOOD; CHILD; DNA METHYLATION; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; METABOLISM; METHODOLOGY; MIDDLE AGED; PRESCHOOL CHILD; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; VALIDATION STUDY;

ADOLESCENT; ADULT; AGE FACTORS; AGED; CHILD; CHILD, PRESCHOOL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA; DNA METHYLATION; HUMANS; MIDDLE AGED; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; YOUNG ADULT;

EID: 67650951482     PISSN: 14690667     EISSN: None     Source Type: Journal    
DOI: 10.1255/ejms.1007     Document Type: Article

References (30)

1. K.D. Robertson, "DNA methylation and human disease", Nat. Rev. Genet. 6, 597 (2005). doi: 10.1038/nrg1655
2. A.S. Yang, M.R.H. Esteâcio, K. Doshi, H. Kondo, E.H. Tajara and J.P.J. Issa, "A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements", Nucl. Acids Res. 32, e38 (2004). doi: 10.1093/nar/gnh032
3. B. Richardson, "Impact of aging on DNA methylation", Ageing Res. Rev. 2, 245 (2003). doi: 10.1016/S1568-1637(03)00010-2
4. vy/ a mice", FASEB J. 12, 949 (1998).
5. B.F. Vanyushin, L.E. Nemirovsky, V.V. Klimenko, V.K. Vasiliev and A.N. Belozersky, "The 5-methylcytosine in DNA of rats. Tissue and age specificity and the changes induced by hydrocortisone and other agents", Gerontologia 19, 138(1973).
6. B. Kwabi-Addo, W. Chung, L. Shen and M. Ittmann, "Age-related DNA methylation changes in normal human prostate tissues", Clin. Cancer Res. 13, 3796 (2007). doi: 10.1158/1078-0432.CCR-07-0085
7. R.M. Kok, D.E.C. Smith, R. Barto, A.M.W. Spijkerman, T. Teerlink, H.J. Gellekink, C. Jakobs and Y.M. SmuLders, "Global DNA methylation measured by liquid chromatog- raphy-tandem mass spectrometry: analytical technique, reference values and determinants in healthy subjects", Clin. Chem. Lab. Med. 45, 903 (2007). doi: 10.1515/ CCLM.2007.137
8. C. Fuke, M. Shimabukero, A. Petronis, J. Sugimoto, T. Oda, K. Miura, T. Miyazaki, C. Ogura, Y. Okazaki and Y. Jinno, "Age related changes in 5-methylcytosine content in human peripheral leukocytes and placentas: an HPLC-based study", Ann. Hum. Genet. 68, 196 (2004). doi: 10.104-6/J.1529-8817.2004..00081.X
9. M.F. Fraga, E. Ballestar, M.F. Paz, S. Ropero and F. Setien, "Epigenetic differences arise during the lifetime of monozygotic twins", Proc. Natl. Acad. Sci. USA 102, 10604 (2005). doi: 10.1073/pnas.0500398102
10. L.G. Song, S.R. James, L. Kazim and A.R. Karpf, "Specific method for the determination of genomic DNA methylation by liquid chromatography- electrospray ionization tandem mass spectrometry", Anal. Chem. 77, 504 (2005). doi: 10.1021/ac0489420
11. M. Ehrkuch, "CpG island hypermethylation and tumor suppressor genes: a booming present, a brighter future", Oncogene 21, 5427 (2002). doi: 10.1038/sj.onc.1205600
12. A.S. Romerio, G. Fiorillo, I. Terruzzi, P. Senesi, G. Testolin and A.Battezzati, "Measurement of DNA methylation using stable isotope dilution and gas chromatography-mass spectrometry", Anal. Biochem. 336,158 (2005). doi: 10.1016/j.ab.2004.09.034
13. M. Wirtz, D. Stach, H.C. Kliem, M. Wiessler and O.J. Schmitz, "Determination of the DNA methylation level in tumor cells by capillary electrophoresis and laser-induced fluorescence detection", Electrophoresis 25, 839 (2004). doi: 10.1002/elps.200305761
14. D.H. Fisher and R.W. Giese, "Determination of 5-methylcytosine in DNA by gas chromatography-electron-capture detection", J. Chromatogr. 452, 51 (1988). doi: 10.1016/S0021-9673[01)81436-9
15. B.H. Ramsahoye, "Measurement of genome wide DNA methylation by reversed-phase high-performance liquid chromatography", Methods 27, 156 (2002). doi: 10.1016/ S1046-2023(02)00069-5
16. M.L. Gonzalgo and P.A. Jones, "Quantitative methylation analysis using methylation-sensitive single-nucleotide primer extension (Ms-SNuPE)", Methods 27, 128 (2002). doi:10.1016/S1046-2023(02)00064-6
17. H.M. Ma, W.B. Zhang, R.F. Song, H.Y. Chen, G.S. Sheng, Z. Zhou and J.M. Fu. "Characterization of methylated and unmethlyated CpG oligodeoxynucleotides by elec-trospray ionization tandem mass spectrometry", Eur. J. Mass. Spectrom. 11, 581 (2005). doi: 10.1255/ejms.774
18. R.F. Song, W.B. Zhang, H.Y. Chen, H.M. Ma, Y.L. Dong, G.Y. Sheng, Z. Zhou and J.M. Fu, "Characterization of G-rich and T-rich oligonucleotides using ion-pair reversed-phase high-performance liquid chromatography/tandem electrospray ionization mass spectrometry", Eur. J. Mass Spectrom. 10, 705 (2004). doi: 10.1255/ejms.666
19. H.Y. Chen, W.B. Zhang, R.F. Song, H.M. Ma, Y.L. Dong, G.Y. Sheng, Z. Zhou and J.M. Fu, "Analysis of DNA methylation by tandem ion-pair reversed-phase high-performance liquid chromatography/electrospray ionization mass spectrometry", Rapid Commun. Mass Spectrom. 18, 2773 (2004). doi: 10.1002/rcm.1675
20. R.F. Song, W.B. Zhang, H.Y. Chen, H.M. Ma, Y.L. Dong, G.Y. Sheng, Z. Zhou and J.M. Fu, "Site determination of phenyl glycidyl ether-DNA adducts using high-performance liquid chromatography with electro-spray ionization tandem mass spectrometry", Rapid Commun. Mass Spectrom. 19, 1120 (2005). doi: 10.1002/ rem.1893
21. S. Friso, S.W. Choi, G.G. Dolnikowski and J. Selhub, "A method to assess genomic DNA methylation using high-performance liquid chromatography/ electrospray ionization mass spectrometry", Anal. Chem. 74, 4526 (2002). doi: 10.1021/ac020050h
22. H.H. Kim, J.H. Park, K.S. Jeong and S. Lee, "Determining the global DNA methylation status of rat according to the identifier repetitive elements", Electrophoresis 28, 3854 (2007). doi: 10.1002/ elps.200600733
23. M.I. Pnayiotidis, R.C. Rancourt, A. Pappa and C.W. White, Effect of cell cycle growth arrest on global DNA methylation status in human lung epithelial-like (A549) cells", In Vivo 20, 861 (2006).
24. B.F. Vanyushin, S.G. Tkacheva and A.N. Belozersky, "Rare bases in animal DNA", Nature 225, 948 (1970). doi: 10.1038/225948a0
25. LP. Pogribny, A.G. Basnakian, B.J. Miller, N.G. Lopatina, L.A. Poirier and S.J. James. "Breaks in genomic DNA and within the p53 gene are associated with hypomethylation in livers of folate/methyl-deficient rats", Cancer Res. 55, 1894(1995).
26. E. Cornacchia, J. Golbus, J. Maybaum, J. Strahler, S. Hanash and B. Richardson. "Hydralazine and procainamide inhibit T cell DNA methylation and induce autoreactivity", J. Immunol. 140, 2197 (1988).
27. B.C. Richardson, "Role of DNA methylation in the regulation of cell function: autoimmunity, aging and cancer", J. Nutr. 132, 2401s (2002).
28. Z. Zhang, C. Deng, Q. Lu and B. Richardson, "Age-dependent DNA methylation changes in the ITGAL (CD11a) promoter", Mech. Ageing Dev. 123, 1257 (2002).
29. R.A. Miller, S. Hanash and B. Richardson, "Unexpected effects of a heterozygous dnmtl null mutation on age-dependent DNA hypomethylation and autoimmunity", J. Gerontol. A Biol. Sci. Med. Sci. 56, B268 (2001).
30. N. Lopatina, J.F. Haskell, L.G. Andrews, J.C. Poole, S. Saldanha and T. Tollefsbol, "Differential maintenance and de novo methylating activity by three DNA methyltransferases in aging and immortalized fibroblasts", J. Cell. Biochem. 84, 324 (2002). doi: 10.1002/jcb.10015 31. C.J. Sherrand R.A. DePinho, "Cellular senescence: mitotic clock or culture shock?", Cell 102, 407 [2000). doi: 10.1016/S0092-8674(00) 00046-5