Functions of phenylalanine residues within the β-barrel stem of the anthrax toxin pore

PLoS ONE

Volumn 4, Issue 7, 2009, Pages

  Wang, Jie ^a   Vernier, Gregory ^a   Fischer, Audrey ^a   Collier, R John ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRAX TOXIN; PHENYLALANINE; BACTERIAL ANTIGEN; BACTERIAL TOXIN;

ARTICLE; BACILLUS ANTHRACIS; BACTERIAL VIRULENCE; CHANNEL GATING; CONTROLLED STUDY; CYTOTOXICITY; MEMBRANE POTENTIAL; MUTAGENESIS; NONHUMAN; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; CHEMISTRY; PHYSIOLOGY; PROTEIN CONFORMATION;

BACILLUS ANTHRACIS;

ANTIGENS, BACTERIAL; BACTERIAL TOXINS; PHENYLALANINE; PROTEIN CONFORMATION;

EID: 67650917390     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0006280     Document Type: Article

References (13)

1. Young JA, Collier RJ (2007) Anthrax toxin: receptor binding, internalization, pore formation, and translocation. Annu Rev Biochem 76: 243-265.
2. Krantz BA, Finkelstein A, Collier RJ (2006) Protein translocation through the anthrax toxin transmembrane pore is driven by a proton gradient. J Mol Biol 355: 968-979.
3. Benson EL, Huynh PD, Finkelstein A, Collier RJ (1998) Identification of residues lining the anthrax protective antigen channel. Biochemistry 37: 3941-3948.
4. Katayama H, Janowiak BE, Brzozowski M, Juryck J, Falke S, et al. (2008) GroEL as a molecular scaffold for structural analysis of the anthrax toxin pore. Nat Struct Mol Biol 15: 754-760.
5. Krantz BA, Melnyk RA, Zhang S, Juris SJ, Lacy DB, et al. (2005) A phenylalanine clamp catalyzes protein translocation through the anthrax toxin pore. Science 309: 777-781.
6. Sun J, Vernier G, Wigelsworth DJ, Collier RJ (2007) Insertion of anthrax protective antigen into liposomal membranes: effects of a receptor. J Biol Chem 282: 1059-1065.
7. Zhang S, Finkelstein A, Collier RJ (2004) Evidence that translocation of anthrax toxin's lethal factor is initiated by entry of its N terminus into the protective antigen channel. Proc Natl Acad Sci U S A 101: 16756-16761.
8. Sellman BR, Nassi S, Collier RJ (2001) Point mutations in anthrax protective antigen that block translocation. J Biol Chem 276: 8371-8376.
9. Aulinger BA, Roehrl MH, Mekalanos JJ, Collier RJ, Wang JY (2005) Combining anthrax vaccine and therapy: a dominant-negative inhibitor of anthrax toxin is also a potent and safe immunogen for vaccines. Infect Immun 73: 3408-3414.
10. Vogt J, Schulz GE (1999) The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence. Structure 7: 1301-1309.
11. Schulz GE (1993) Bacterial porins: structure and function. Curr Opin Cell Biol 5: 701-707.
12. Song L, Hobaugh MR, Shustak C, Cheley S, Bayley H, Gouaux JE (1996) Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore. Science 274: 1859-1866.
13. Iacovache I, Paumard P, Scheib H, Lesieur C, Sakai N, et al. (2006) A rivet model for channel formation by aerolysin-like pore-forming toxins. EMBO J 25: 457-466.