Quantification of water and biomass in small colony variant PAO1 biofilms by confocal Raman microspectroscopy

Applied Microbiology and Biotechnology

Volumn 83, Issue 6, 2009, Pages 1171-1182

  Sandt, Christophe ^a,b   Smith Palmer, Truis ^a   Comeau, Jonathan ^a   Pink, David ^a,b  

^a ST FRANCIS XAVIER UNIVERSITY   (Canada)

^b Networks of Centres of Excellence   (Canada)

Author keywords

Bacteria; Biomass; Confocal Raman microscopy; Hydration; Map

Indexed keywords

BIOMASS DENSITY; BIOMASS RATIO; CALIBRATION CURVES; CELL DENSITY; CONFOCAL RAMAN MICROSCOPY; CONFOCAL RAMAN MICROSPECTROSCOPY; IN-SITU; PROTEIN SOLUTION; PSEUDOALTEROMONAS; PSEUDOMONAS AERUGINOSA; RAMAN SPECTRA; STRETCHING BANDS; STRETCHING VIBRATIONS; WILD TYPES;

BACTERIOLOGY; BIOFILMS; BIOFILTERS; CELL MEMBRANES; HYDRATION; RAMAN SPECTROSCOPY; STRETCHING; SUSPENSIONS (FLUIDS); WATER CONTENT;

BIOMASS;

BACTERIUM; BIOFILM; BIOMASS; CALIBRATION; HYDRATION; MAPPING; PROTEIN; QUANTITATIVE ANALYSIS; RAMAN SPECTROSCOPY; WATER CONTENT;

ANALYTIC METHOD; ARTICLE; BACTERIAL STRAIN; BACTERIUM COLONY; BIOFILM; BIOMASS; CALIBRATION; CELL DENSITY; CONFOCAL RAMAN MICROSPECTROSCOPY; CONTROLLED STUDY; HYDRATION; NONHUMAN; PSEUDOALTEROMONAS; PSEUDOMONAS AERUGINOSA; QUANTITATIVE ANALYSIS; RAMAN SPECTROMETRY; SUSPENSION; WATER CONTENT; WILD TYPE;

BIOFILMS; BIOMASS; COLONY COUNT, MICROBIAL; PSEUDOALTEROMONAS; PSEUDOMONAS AERUGINOSA; SPECTRUM ANALYSIS, RAMAN; WATER;

BACTERIA (MICROORGANISMS); PSEUDOALTEROMONAS SP.; PSEUDOMONAS AERUGINOSA; STRAWBERRY CRINKLE VIRUS;

EID: 67650713575     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2072-y     Document Type: Article

References (22)

1. PJ Aarnoutse JA Westerhuis 2005 Quantitative Raman reaction monitoring using the solvent as internal standard Anal Chem 77 1228 1236 (Pubitemid 40327859)
2. LP Choo-Smith K Maquelin T van Vreeswijk HA Bruining GJ Puppels NA Ngo-Thi C Kirschner D Naumann D Ami AM Villa F Orsini SM Doglia H Lamfarraj DG Sockalingum M Manfait P Allouch HP Endtz 2001 Investigating microbial (micro) colony heterogeneity by vibrational spectroscopy Appl Environ Microbiol 67 1461 1469 (Pubitemid 33644909)
3. E Déziel Y Comeau R Villemur 2001 Initiation of biofilm formation by Pseudomonas aeruginosa 57RP correlates with emergence of hyperpiliated and highly adherent phenotypic variants deficient in swimming, swarming, and twitching motilities J Bacteriol 183 1195 1204 (Pubitemid 32107715)
4. N Everall 2004 Depth profiling with confocal Raman microscopy, part I Spectroscopy 19 22 33
5. N Everall 2004 Depth profiling with confocal Raman microscopy, part II Spectroscopy 19 16 27 (Pubitemid 41100944)
6. S Häußler 2004 Biofilm formation by the small colony variant phenotype of Pseudomonas aeruginosa Environ Microbiol 6 546 551 (Pubitemid 38708689)
7. MJ Kirisits L Prost M Starkey MR Parsek 2005 Characterization of colony morphology variants isolated from Pseudomonas aeruginosa biofilms Appl Environ Microbiol 71 4809 4821 (Pubitemid 41129540)
8. K Maquelin C Kirschner LP Choo-Smith NA Ngo-Thi T van Vreeswijk M Stammler HP Endtz HA Bruining D Naumann GJ Puppels 2003 Prospective study of the performance of vibrational spectroscopies for rapid identification of bacterial and fungal pathogens recovered from blood cultures J Clin Microbiol 41 324 329 (Pubitemid 36098595)
9. L Marcotte H Therien-Aubin C Sandt J Barbeau M Lafleur 2004 Solute size effects on the diffusion in biofilms of Streptococcus mutans Biofouling 20 189 201 (Pubitemid 40084532)
10. L Marcotte J Barbeau M Lafleur 2004 Characterization of the diffusion of polyethylene glycol in Streptococcus mutans biofilms by Raman microspectroscopy Appl Spectrosc 58 1295 1301
11. TR Neu GDW Swernhone JR Lawrence 2001 Assessment of lectin binding analysis for in situ detection of glycoconjugates in biofilms systems Microbiology 147 299 313 (Pubitemid 32156806)
12. DE Nivens DE Ohman J Williams MJ Franklin 2001 Role of alginate and its O-acetylation in formation of Pseudomonas aeruginosa microcolonies and biofilms J Bacteriol 183 1047 1057 (Pubitemid 32095315)
13. R Pätzold M Keuntje A Anders-von Ahlften 2006 A new approach to non-destructive analysis of biofilms by confocal Raman microscopy Anal Bioanal Chem 386 286 292 (Pubitemid 44334902)
14. MJ Pelletier 2003 Quantitative analysis using Raman spectrometry Appl Spectrosc 57 20A 42A
15. B Purevdorj-Gage WJ Costerton P Stoodley 2005 Phenotypic differentiation and seeding dispersal in non-mucoid and mucoid Pseudomonas aeruginosa biofilms Microbiology 151 1569 1576 (Pubitemid 40711803)
16. C Sandt T Smith-Palmer J Pink L Brennan DA Pink 2007 Confocal Raman microspectroscopy as a tool for studying the chemical heterogeneities of biofilms in situ J Appl Microbiol 103 1808 1820 (Pubitemid 47620567)
17. C Sandt T Smith-Palmer J Pink DA Pink 2008 Quantification of local water and biomass in wild type PAO1 biofilms by confocal Raman microspectroscopy J Microbiol Meth 75 148 152
18. M Schneider K Mühlemann S Droz S Couzinet C Casaulta S Zimmerli 2008 Clinical characteristics associated with isolation of small-colony variants of Staphylococcus aureus and Pseudomonas aeruginosa from respiratory secretions of patients with cystic fibrosis J Clin Microbiol 46 1832 1834 (Pubitemid 351657236)
19. C Staudt H Horn DC Hempel TR Neu 2004 Volumetric measurements of bacterial cells and extracellular polymeric substance glycoconjugates in biofilms Biotechnol Bioeng 88 585 592 (Pubitemid 39656806)
20. PA Suci GG Geesey BJ Tyler 2001 Integration of Raman microspectroscopy, differential interference contrast microscopy, and attenuated total reflection Fourier transform infrared spectroscopy to investigate chlorhexidine spatial and temporal distribution in Candida albican J Microbiol Meth 46 193 208 (Pubitemid 32579208)
21. JS Webb M Lau S Kjellberg 2004 Bacteriophage and phenotypic variation in Pseudomonas aeruginosa biofilm development J Bacteriol 186 8066 8073 (Pubitemid 39552518)
22. R Weiner S Langille E Quintero 1995 Structure, function and immunochemistry of bacterial exopolysaccharides J Ind Microbiol 15 339 346 (Pubitemid 26022308)