Multiple genotypic changes in hypersusceptible strains of Pseudomonas aeruginosa isolated from cystic fibrosis patients do not always correlate with the phenotype

Journal of Antimicrobial Chemotherapy

Volumn 64, Issue 2, 2009, Pages 294-300

  Wolter, Daniel J ^a,b   Black, Jennifer A ^a   Lister, Philip D ^a   Hanson, Nancy D ^a  

^a CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

AmpC; Efflux; OprD; OprF; P. aeruginosa

Indexed keywords

ANTIMETABOLITE; AZTREONAM; BACTERIAL PROTEIN; BETA LACTAM ANTIBIOTIC; BETA LACTAMASE; BETA LACTAMASE AMPC; CARBAPENEM; CEFEPIME; CEFOXITIN; CEFTAZIDIME; CEPHALOSPORINASE; CIPROFLOXACIN; COTRIMOXAZOLE; IMIPENEM; MEROPENEM; OUTER MEMBRANE PROTEIN; PIPERACILLIN; PIPERACILLIN PLUS TAZOBACTAM; PORIN; PROTEIN GLMS; PROTEIN MEXAB; PROTEIN MEXCD; PROTEIN MEXEF; PROTEIN MEXXY; PROTEIN OPRD; PROTEIN OPRJ; PROTEIN OPRM; PROTEIN OPRN; QUINOLINE DERIVED ANTIINFECTIVE AGENT; UNCLASSIFIED DRUG; UNINDEXED DRUG;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; CYSTIC FIBROSIS; DOWN REGULATION; GENE OVEREXPRESSION; GENOTYPE; HUMAN; HYDROLYSIS; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR INTERACTION; NONHUMAN; OPERON; PHENOTYPE; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA;

ANTI-BACTERIAL AGENTS; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BETA-LACTAMASES; CYSTIC FIBROSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION PROFILING; HUMANS; MEMBRANE TRANSPORT PROTEINS; MICROBIAL SENSITIVITY TESTS; PHENOTYPE; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SALIVA;

EID: 67650711671     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkp185     Document Type: Article

References (39)

1. Nikaido H. Preventing drug access to targets: Cell surface permeability barriers and active efflux in bacteria. Semin Cell Dev Biol 2001; 12: 215-23.
2. Jones RN. Important and emerging β-lactamase-mediated resistances in hospital-based pathogens: The Amp C enzymes. Diagn Microbiol Infect Dis 1998; 31: 461-6.
3. Lister PD, Gardner VM, Sanders CC. Clavulanate induces expression of the Pseudomonas aeruginosa AmpC cephalosporinase at physiologically relevant concentrations and antagonizes the antibacterial activity of ticarcillin. Antimicrob Agents Chemother 1999; 43: 882-9.
4. Kohler T, Kok M, Michea-Hamzehpour M et al. Multidrug efflux in intrinsic resistance to trimethoprim and sulfamethoxazole in Pseudomonas aeruginosa. Antimicrob Agents Chemother 1996; 40: 2288-90.
5. Li XZ, Nikaido H, Poole K. Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa. Antimicrob Agents Chemother 1995; 39: 1948-53.
6. Jeannot K, Sobel ML, El Garch F et al. Induction of the MexXY efflux pump in Pseudomonas aeruginosa is dependent on drug-ribosome interaction. J Bacteriol 2005; 187: 5341-6.
7. Masuda N, Sakagawa E, Ohya S et al. Contribution of the MexX-MexY-OprM efflux system to intrinsic resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother 2000; 44: 2242-6.
8. Angus BL, Carey AM, Caron DA et al. Outer membrane permeability in Pseudomonas aeruginosa: Comparison of a wild-type with an antibiotic-supersusceptible mutant. Antimicrob Agents Chemother 1982; 21: 299-309.
9. Kropinski AM, Kuzio J, Angus BL et al. Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic-supersusceptible mutant of Pseudomonas aeruginosa. Antimicrob Agents Chemother 1982; 21: 310-9.
10. Ramos-Aires J, Plesiat P, Kocjancic-Curty L et al. Selection of an antibiotic-hypersusceptible mutant of Pseudomonas aeruginosa: identification of the GlmR transcriptional regulator. Antimicrob Agents Chemother 2004; 48: 843-51.
11. Raetz CRH. Escherichia coli and Salmonella: Cellular and Molecular Biology. Washington, DC: ASM Press, 1996.
12. Morita Y, Kimura N, Mima T et al. Roles of MexXY- and MexAB-multidrug efflux pumps in intrinsic multidrug resistance of Pseudomonas aeruginosa PAO1. J Gen Appl Microbiol 2001; 47 27-32.
13. Okamoto K, Gotoh N, Nishino T. Pseudomonas aeruginosa reveals high intrinsic resistance to penem antibiotics: Penem resistance mechanisms and their interplay. Antimicrob Agents Chemother 2001; 45: 1964-71.
14. Masuda N, Gotoh N, Ishii C et al. Interplay between chromosomal β-lactamase and the MexAB-OprM efflux system in intrinsic resistance to β-lactams in Pseudomonas aeruginosa. Antimicrob Agents Chemother 1999; 43: 400-2.
15. May JR, Ingold A. Sensitivity of respiratory strains of Pseudomonas aeruginosa to carbenicillin. J Med Microbiol 1973; 6: 77-82.
16. Irvin RT, Govan JW, Fyfe JA et al. Heterogeneity of antibiotic resistance in mucoid isolates of Pseudomonas aeruginosa obtained from cystic fibrosis patients: Role of outer membrane proteins. Antimicrob Agents Chemother 1981; 19: 1056-63.
17. Lechtzin N, John M, Irizarry R et al. Outcomes of adults with cystic fibrosis infected with antibiotic-resistant Pseudomonas aeruginosa. Respiration 2006; 73: 27-33.
18. Merlo CA, Boyle MP, Diener-West M et al. Incidence and risk factors for multiple antibiotic-resistant Pseudomonas aeruginosa in cystic fibrosis. Chest 2007; 132: 562-8.
19. Fyfe JA, Govan JR. Chromosomal loci associated with antibiotic hypersensitivity in pulmonary isolates of Pseudomonas aeruginosa. J Gen Microbiol 1984; 130: 825-34.
20. Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically - Seventh Edition: Approved Standard M7-A7. CLSI, Wayne, PA, USA, 2006.
21. Goering RV. Pulsed-field gel electrophoresis. In: Persing DH, Tenover FC, Versalovic J et al., eds. Molecular Microbiology: Diagnostic Principles and Practice. Washington, DC: ASM Press, 2004; 185-96.
22. Wolter DJ, Hanson ND, Lister PD. AmpC and OprD are not involved in the mechanism of imipenem hypersusceptibility among Pseudomonas aeruginosa isolates overexpressing the mexCD-oprJ efflux pump. Antimicrob Agents Chemother 2005; 49: 4763-6.
23. Wolter DJ, Smith-Moland E, Goering RV et al. Multidrug resistance associated with mexXY expression in clinical isolates of Pseudomonas aeruginosa from a Texas hospital. Diagn Microbiol Infect Dis 2004; 50: 43-50.
24. Zhao Q, Li XZ, Srikumar R et al. Contribution of outer membrane efflux protein OprM to antibiotic resistance in Pseudomonas aeruginosa independent of MexAB. Antimicrob Agents Chemother 1998; 42: 1682-8.
25. Sanders CC, Sanders WE Jr. Type I β-lactamases of gram-negative bacteria: Interactions with β-lactam antibiotics. J Infect Dis 1986; 154: 792-800.
26. Piddock LJ, Hall MC, Bellido F et al. A pleiotropic, posttherapy, enoxacin-resistant mutant of Pseudomonas aeruginosa. Antimicrob Agents Chemother 1992; 36: 1057-61.
27. Woodruff WA, Hancock RE. Construction and characterization of Pseudomonas aeruginosa protein F-deficient mutants after in vitro and in vivo insertion mutagenesis of the cloned gene. J Bacteriol 1988; 170: 2592-8.
28. Malhotra S, Silo-Suh LA, Mathee K et al. Proteome analysis of the effect of mucoid conversion on global protein expression in Pseudomonas aeruginosa strain PAO1 shows induction of the disulfide bond isomerase, dsbA. J Bacteriol 2000; 182: 6999-7006.
29. Trias J, Nikaido H. Outer membrane protein D2 catalyzes facilitated diffusion of carbapenems and penems through the outer membrane of Pseudomonas aeruginosa. Antimicrob Agents Chemother 1990; 34: 52-7.
30. Kohler T, Michea-Hamzehpour M, Epp SF et al. Carbapenem activities against Pseudomonas aeruginosa: Respective contributions of OprD and efflux systems. Antimicrob Agents Chemother 1999; 43: 424-7.
31. Poole K, Srikumar R. Multidrug efflux in Pseudomonas aeruginosa components, mechanisms and clinical significance. Curr Top Med Chem 2001; 1: 59-71.
32. Li XZ, Barre N, Poole K. Influence of the MexA-MexB-OprM multidrug efflux system on expression of the MexC-MexD-OprJ and MexE-MexF-OprN multidrug efflux systems in Pseudomonas aeruginosa. J Antimicrob Chemother 2000; 46: 885-93.
33. Jo JT, Brinkman FS, Hancock RE. Aminoglycoside efflux in Pseudomonas aeruginosa: Involvement of novel outer membrane proteins. Antimicrob Agents Chemother 2003; 47: 1101-11.
34. Hocquet D, Nordmann P, El Garch F et al. Involvement of the MexXY-OprM efflux system in emergence of cefepime resistance in clinical strains of Pseudomonas aeruginosa. Antimicrob Agents Chemother 2006; 50: 1347-51.
35. Poole K, Gotoh N, Tsujimoto H et al. Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa. Mol Microbiol 1996; 21: 713-24.
36. Lister PD. Chromosomally-encoded resistance mechanisms of Pseudomonas aeruginosa: Therapeutic implications. Am J Pharmacogenomics 2002; 2: 235-43.
37. Holtje JV, Kopp U, Ursinus A et al. The negative regulator of β-lactamase induction AmpD is a N-acetyl-anhydromuramyl-L-alanine amidase. FEMS Microbiol Lett 1994; 122: 159-64.
38. Juan C, Moya B, Perez JL et al. Stepwise upregulation of the Pseudomonas aeruginosa chromosomal cephalosporinase conferring high-level β-lactam resistance involves three AmpD homologues. Antimicrob Agents Chemother 2006; 50: 1780-7.
39. Korfmann G, Sanders CC. ampG is essential for high-level expression of AmpC β-lactamase in Enterobacter cloacae. Antimicrob Agents Chemother 1989; 33: 1946-51.