A serum- and feeder-free technique of culturing human corneal epithelial stem cells on amniotic membrane

Molecular Vision

Volumn 15, Issue , 2009, Pages 1294-1302

  Lekhanont, Kaevalin ^a   Choubtum, Lulin ^a   Chuck, Roy S ^b   Sa ngiampornpanit, Tarinee ^a   Chuckpaiwong, Varintorn ^a   Vongthongsri, Anun ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BREAST CANCER RESISTANCE PROTEIN; CONNEXIN 43; CYTOKERATIN 12; CYTOKERATIN 15; CYTOKERATIN 19; CYTOKERATIN 3; PROTEIN P63; PROTEIN P75;

AMNION; ARTICLE; CADAVER DONOR; CELL ASSAY; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL SIZE; CELL STRUCTURE; CONTAMINATION; CONTROLLED STUDY; CORNEA EPITHELIUM; CULTURE MEDIUM; EXPLANT; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; MONOLAYER CULTURE; PHASE CONTRAST MICROSCOPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RISK REDUCTION; STEM CELL;

AGED; AMNION; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CULTURE MEDIA; CULTURE MEDIA, SERUM-FREE; EPITHELIUM, CORNEAL; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; MIDDLE AGED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELLS;

EID: 67650663212     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (51)

1. Schermer A, Galvin S, Sun TT. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 1986; 103:49-62.
2. Cotsarelis G, Cheng SZ, Dong G, Sun TT, Lavker RM. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell 1989; 57:201-9.
3. Thoft RA, Friend J. The X, Y, Z hypothesis of corneal epithelial maintenance. Invest Ophthalmol Vis Sci 1983; 24:1442-3.
4. Buck RC. Measurement of centripetal migration of normal corneal epithelial cells in the mouse. Invest Ophthalmol Vis Sci 1985; 26:1296-9.
5. Chen JJ, Tseng SC. Abnormal corneal epithelial wound healing in partial-thickness removal of limbal epithelium. Invest Ophthalmol Vis Sci 1991; 32:2219-33.
6. Kenyon KR, Tseng SC. Limbal autograft transplantation for ocular surface disorders. Ophthalmology 1989; 96:709-22.
7. Tseng SC, Prabhasawat P, Barton K, Gray T, Meller D. Amniotic membrane transplantation with or without limbal allografts for corneal surface reconstruction in patients with limbal stem cell deficiency. Arch Ophthalmol 1998; 116:431-41.
8. Tsubota K, Satake Y, Kaido M, Shinozaki N, Shimmura S, Bissen-Miyajima H, Shimazaki J. Treatment of severe ocular-surface disorders with corneal epithelial stem-cell transplantation. N Engl J Med 1999; 340:1697-703.
9. Holland EJ, Schwartz GS. Changing concepts in the management of severe ocular surface disease over twenty-five years. Cornea 2000; 19:688-98.
10. Holland EJ, Schwartz GS. Epithelial stem-cell transplantation for severe ocular-surface disease. N Engl J Med 1999; 340:1752-3.
11. Holland EJ, Djalilian AR, Schwartz GS. Management of aniridic keratopathy with keratolimbal allograft: a limbal stem cell transplantation technique. Ophthalmology 2003; 110:125-30.
12. Solomon A, Ellies P, Anderson DF, Touhami A, Grueterich M, Espana EM, Ti SE, Goto E, Feuer WJ, Tseng SC. Long-term outcome of keratolimbal allograft with or without penetrating keratoplasty for total limbal stem cell deficiency. Ophthalmology 2002; 109:1159-66.
13. Jabs DA, Rosenbaum JT, Foster CS, Holland GN, Jaffe GJ, Louie JS, Nussenblatt RB, Stiehm ER, Tessler H, Van Gelder RN, Whitcup SM, Yocum D. Guidelines for the use of immunosuppressive drugs in patients with ocular inflammatory disorders: recommendations of an expert panel. Am J Ophthalmol 2000; 130:492-513.
14. Pellegrini G, Traverso CE, Franzi AT, Zingirian M, Cancedda R, De Luca M. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 1997; 349:990-3.
15. Tsai RJ, Li LM, Chen JK. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med 2000; 343:86-93.
16. Schwab IR, Reyes M, Isseroff RR. Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease. Cornea 2000; 19:421-6.
17. Koizumi N, Fullwood N, Bairaaktaris G, Inatomi T, Kinoshita S, Quantock A. Cultivation of corneal epithelial cells on intact and denuded human amniotic membrane. Invest Ophthalmol Vis Sci 2000; 41:2506-13.
18. Koizumi N, Inatomi T, Suzuki T, Sotozono C, Kinoshita S. Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology 2001; 108:1569-74.
19. Rama P, Bonini S, Lambiase A, Golisano O, Paterna P, De Luca M, Pellegrini G. Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. Transplantation 2001; 72:1478-85.
20. Grueterich M, Espana EM, Touhami A, Ti SE, Tseng SC. Phenotypic study of a case with successful transplantation of ex vivo expanded human limbal epithelium for unilateral total limbal stem cell deficiency. Ophthalmology 2002; 109:1547-52.
21. Shimazaki J, Aiba M, Goto E, Kato N, Shimmura S, Tsubota K. Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders. Ophthalmology 2002; 109:1285-90.
22. Sangwan VS, Matalia HP, Vemuganti GK, Ifthekar G, Fatima A, Singh S, Rao GN. Early results of penetrating keratoplasty after cultivated limbal epithelium transplantation. Arch Ophthalmol 2005; 123:334-40.
23. Sangwan VS, Matalia HP, Vemuganti GK, Fatima A, Ifthekar G, Singh S, Nutheti R, Rao GN. Clinical outcome of autologous cultivated limbal epithelium transplantation. Indian J Ophthalmol 2006; 54:29-34.
24. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975; 6:331-43.
25. Talbot M, Carrier P, Giasson CJ, Deschambeault A, Guérin SL, Auger FA, Bazin R, Germain L. Autologous transplantation of rabbit limbal epithelia cultured on fibrin gels for ocular surface reconstruction. Mol Vis 2006; 12:65-75.
26. Han B, Schwab IR, Madsen TK, Isseroff RR. A fibrin-based bioengineered ocular surface with human corneal epithelial stem cells. Cornea 2002; 21:505-10.
27. Nishida K, Yamato M, Hayashida Y, Watanabe K, Yamamoto K, Adachi E, Nagai S, Kikuchi A, Maeda N, Watanabe H, Okano T, Tano Y. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N Engl J Med 2004; 351:1187-96.
28. Nishida K, Yamato M, Hayashida Y, Watanabe K, Maeda N, Watanabe H, Yamamoto K, Nagai S, Kikuchi A, Tano Y, Okano T. Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface. Transplantation 2004; 77:379-85.
29. Francis D, Abberton K, Thompson E, Daniell M. Myogel supports the ex-vivo amplification of corneal epithelial cells. Exp Eye Res 2009; 88:339-46.
30. Meller D, Pires RT, Tseng SC. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br J Ophthalmol 2002; 86:463-71.
31. Grueterich M, Espana EM, Tseng SC. Ex vivo expansion of limbal epithelial stem cells: amniotic membrane serving as a stem cell niche. Surv Ophthalmol 2003; 48:631-46.
32. Grueterich M, Espana EM, Tseng SC. Modulation of keratin and connexin expression in limbal epithelium expanded on denuded amniotic membrane with and without a 3T3 fibroblast feeder layer. Invest Ophthalmol Vis Sci 2003; 44:4230-6.
33. Tseng SC, Meller D, Anderson DF, Touhami A, Pires RT, Grüterich M, Solomon A, Espana E, Sandoval H, Ti SE, Goto E. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane for treating corneal diseases with total limbal stem cell deficiency. Adv Exp Med Biol 2002; 506:1323-34.
34. Koizumi N, Rigby H, Fullwood NJ, Kawasaki S, Tanioka H, Koizumi K, Kociok N, Joussen AM, Kinoshita S. Comparison of intact and denuded amniotic membrane as a substrate for cell-suspension culture of human limbal epithelial cells. Graefes Arch Clin Exp Ophthalmol 2007; 245:123-34.
35. Koizumi N, Inatomi T, Quantock AJ, Fullwood NJ, Dota A, Kinoshita S. Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits. Cornea 2000; 19:65-71.
36. Choi TH, Tseng SC. In vivo and in vitro demonstration of epithelial cell-induced myofibroblast differentiation of keratocytes and an inhibitory effect by amniotic membrane. Cornea 2001; 20:197-204.
37. Kim JS, Kim JC, Na BK, Jeong JM, Song CY. Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acutecomeal alkali burn Exp Eye Res 2000; 70:329-37.
38. Shimazaki J, Higa K, Morito F, Dogru M, Kawakita T, Satake Y, Shimmura, S, Tsubota K, Factors influencing outcomes in cultivated limbal epithelial transplantation for chronic cicatricial ocular surface disorders. Ain J Ophthalmol 2007; 143:945-53.
39. Nakamura T, Endo K, Cooper LJ, Fullwood NJ, Taniruji N, Tsuzuki M, Koizumi N, Inatomi T, Sano Y, Kinoshita S. The successful culture and autologous transplantation of rabbit oral mucosal epithelial cells on amniotic membrane. Invest Ophthalmol Vis Sci 2003; 44:106-16.
40. Liu S, Li J, Wang C, Tan D, Beuerman R. Human limbal progenitor cell characteristics are maintained in tissue culture. Ann Acad Med Singapore 2006; 35:80-6.
41. Schwab IR, Johnson NT, Harkin DG. Inherent risks associated with manufacture of bioengineered ocular surface tissue. Arch Ophthalmol 2006; 124:1734-40.
42. U.S. Department of Health and Human Services Food and Drug Administration (FDA) Center for Biologics Evaluation and Research. (CBER). Information and Recommendations for Physicians Involved in the Co-culture of Human Embryos with Nonhuman Animal Cells. U.S. Food and Drug Administration: Rockville MD; 2000,
43. Hultman CS, Brinson GM, Siltharm S, deSerres S, Cairns BA, Peterson HD, Meyer AA. Allogeneic fibroblasts used to grow cultured epidermal autografts persist in vivo and sensitize the graft recipient for accelerated second-set rejection. J Trauma 1996; 41:51-8.
44. Boneva RS, Folks TM, Chapman LE. Infectious disease issues in xenotransplantation. Clin Microbiol Rev 2001; 14:1-14.
45. Bredehorn T, Eichhorst A, Tullo A, Duncker G. Ceutzfeldt-Jakob disease: a problem for tissue donation. Transplant Proc 2002; 34:2349-50.
46. Fatima A, Sangwan VS, Iftekhar G, Reddy P, Matalia H, Balasubramanian D, Vemuganti GK. Technique of cultivating limbal derived corneal epithelium on human amniotic membrane for clinical transplantation. J Postgrad Med 2006;52:257-61.
47. Nakamura T, Inatomi T, Sotozono, C, Ang LP, Koizumi N, Yokoi N, Kinoshita S. Transplantation of autologous serum-derived cultivated corneal epithelial equivalents for the treatment of severe ocular surface disease. Ophthalmology 2006;113:1765-12.
48. Nakamura T, Ang LP, Rigby H, Sekiyama E, Inatomi T, Sotozono C, Fullwood NJ, Kinoshita S. The use of autologous serum in the development of corneal and oral epithelial equivalents in patients with Stevens-Johnson syndrome. Invest Ophthalmol Vis Sci 2006; 47:909-16.
49. Teixeira AI, McKie GA, Foley JD, Bertics PJ, Nealey PF, Murphy CJ. The effect of environmental factors on the response of human corneal epithelial cells to nanoscale substrate topography. Biomaterials 2006; 27:3945-54.
50. Kolli S, Lako, M, Figueiredo F, Mudhar H, Ahmad S. Loss of corneal epithelial stem cell properties in outgrowths from human limbal explants cultured on intact amniotic membrane. Regen Med 2008; 3:329-42.
51. Kim HS, Jun Song X, de Paiva CS, Chen Z, Pflugfelder SC, Li DQ. Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures. Exp Eye Res 2004; 79:41-9.