A multiple ATG gene knockout strain for yeast two-hybrid analysis

Autophagy

Volumn 5, Issue 5, 2009, Pages 699-705

  Cao, Yang ^a   Nair, Usha ^a   Yasumura Yorimitsu, Kyoko ^a   Klionsky, Daniel J ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Atg1 complex; Atg29; Atg31; Autophagy; Protein protein interaction

Indexed keywords

ATG PROTEIN; ATG1 PROTEIN; ATG17 PROTEIN; ATG29 PROTEIN; ATG31 PROTEIN; BETA GALACTOSIDASE; CELL PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; AUTOPHAGY; CELL GROWTH; ENZYME ACTIVITY; IMMUNOPRECIPITATION; NONHUMAN; PLASMID; PROMOTER REGION; PROTEIN PROTEIN INTERACTION; REPORTER GENE; SACCHAROMYCES CEREVISIAE; TWO HYBRID SYSTEM; WILD TYPE;

EID: 67650234502     PISSN: 15548627     EISSN: 15548635     Source Type: Journal    
DOI: 10.4161/auto.5.5.8382     Document Type: Article

References (21)

1. Mizushima N, Noda T, Ohsumi Y. Apg16p is required for the function of the Apg12p- Apg5p conjugate in the yeast autophagy pathway. EMBO J 1999; 18:3888-3896 (Pubitemid 29335842)
2. Shintani T, Huang W-P, Stromhaug PE, Klionsky DJ. Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway. Dev Cell 2002; 3:825-837 (Pubitemid 35460783)
3. Cao Y, Cheong H, Song H, Klionsky DJ. In vivo reconstitution of autophagy in Saccharomyces cerevisiae. J Cell Biol 2008; 182:703-713
4. He C, Baba M, Cao Y, Klionsky DJ. Self-interaction is critical for Atg9 transport and function at the phagophore assembly site during autophagy. Mol Biol Cell 2008; 19:5506-5516
5. James P, Halladay J, Craig EA. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 1996; 144:1425-1436 (Pubitemid 26427892)
6. Klionsky DJ. The molecular machinery of autophagy: unanswered questions. J Cell Sci 2005; 118:7-18. (Pubitemid 40277100)
7. Cheong H, Nair U, Geng J, Klionsky DJ. The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae. Mol Biol Cell 2008; 19:668-681 (Pubitemid 351272154)
8. Kawamata T, Kamada Y, Suzuki K, Kuboshima N, Akimatsu H, Ota S, et al. Characterization of a novel autophagy-specific gene, ATG29. Biochem Biophys Res Commun 2005; 338:1884-1889
9. Kabeya Y, Kawamata T, Suzuki K, Ohsumi Y. Cis1/Atg31 is required for autophagosome formation in Saccharomyces cerevisiae. Biochem Biophys Res Commun 2007; 356:405-410
10. Kim J, Kamada Y, Stromhaug PE, Guan J, Hefner-Gravink A, Baba M, et al. Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole. J Cell Biol 2001; 153:381-396 (Pubitemid 34280222)
11. Kamada Y, Funakoshi T, Shintani T, Nagano K, Ohsumi M, Ohsumi Y. Tor-mediated induction of autophagy via an Apg1 protein kinase complex. J Cell Biol 2000; 150:1507-1513 (Pubitemid 30728921)
12. Cheong H, Yorimitsu T, Reggiori F, Legakis JE, Wang C-W, Klionsky DJ. Atg17 regulates the magnitude of the autophagic response. Mol Biol Cell 2005; 16:3438-3453 (Pubitemid 40962611)
13. Yorimitsu T, Klionsky DJ. Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway. Mol Biol Cell 2005; 16:1593-1605 (Pubitemid 40471933)
14. Kanki T, Klionsky DJ. Mitophagy in yeast occurs through a selective mechanism. J Biol Chem 2008; 283:32386-32393
15. Kawamata T, Kamada Y, Kabeya Y, Sekito T, Ohsumi Y. Organization of the preautophagosomal structure responsible for autophagosome formation. Mol Biol Cell 2008.
16. Kabeya Y, Kamada Y, Baba M, Takikawa H, Sasaki M, Ohsumi Y. Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy. Mol Biol Cell 2005; 16:2544-2553 (Pubitemid 40632233)
17. Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH. A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res 2002; 30:23.
18. Boyd C, Hughes T, Pypaert M, Novick P. Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p. J Cell Biol 2004; 167:889-901. (Pubitemid 39628111)
19. He C, Song H, Yorimitsu T, Monastyrska I, Yen W-L, Legakis JE, Klionsky DJ. Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast. J Cell Biol 2006; 175:925-935 (Pubitemid 44969198)
20. Robinson JS, Klionsky DJ, Banta LM, Emr SD. Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 1988; 8:4936-4948
21. Cao Y, Klionsky DJ. New insights into autophagy using a multiple knockout strain. Autophagy 2008; 4:1073-1075