New insights into autophagy using a multiple knockout strain

Autophagy

Volumn 4, Issue 8, 2008, Pages 1073-1075

  Cao, Yang ^a   Klionsky, Daniel J ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Conjugation; In vivo reconstitution; Lysosome; Vacuole; Yeast

Indexed keywords

ARTICLE; AUTOPHAGY; CONTROLLED STUDY; FUNGAL GENE; FUNGAL STRAIN; GENE DELETION; IN VITRO STUDY; MEMBRANE RECONSTITUTION; MOLECULAR DYNAMICS; NONHUMAN; SACCHAROMYCES CEREVISIAE;

HARNESS; MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 58149088974     PISSN: 15548627     EISSN: 15548635     Source Type: Journal    
DOI: 10.4161/auto.6962     Document Type: Article

References (20)

1. Mizushima N. Autophagy: process and function. Genes Dev 2007; 21:2861-73.
2. Rubinsztein DC, Gestwicki JE, Murphy LO, Klionsky DJ. Potential therapeutic applications of autophagy. Nat Rev Drug Discov 2007; 6:304-12.
3. Cao Y, Klionsky DJ. Physiological functions of Atg6/Beclin 1: A unique autophagy-related protein. Cell Res 2007; 17:839-49.
4. Klionsky DJ. The molecular machinery of autophagy: Unanswered questions. J Cell Sci 2005; 118:7-18.
5. Cao Y, Cheong H, Song H, Klionsky DJ. In vivo reconstitution of autophagy in Saccharomyces cerevisiae. J Cell Biol 2008; 182:703-13.
6. Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH. A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res 2002; 30:e23.
7. Kabeya Y, Kawamata T, Suzuki K, Ohsumi Y. Cis1/Atg31 is required for autophagosome formation in Saccharomyces cerevisiae. Biochem Biophys Res Commun 2007; 356:405-10.
8. Yorimitsu T, Klionsky DJ. Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to vacuole targeting pathway. Mol Biol Cell 2005; 16:1593-605.
9. Shintani T, Huang W-P, Stromhaug PE, Klionsky DJ. Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway. Dev Cell 2002; 3:825-37.
10. Scott SV, Guan J, Hutchins MU, Kim J, Klionsky DJ. Cvt19 is a receptor for the cytoplasm-to-vacuole targeting pathway. Mol Cell 2001; 7:1131-41.
11. Suzuki K, Kubota Y, Sekito T, Ohsumi Y. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes Cells 2007; 12:209-18.
12. Mizushima N, Noda T, Yoshimori T, Tanaka Y, Ishii T, George MD, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.
13. Geng J, Klionsky DJ. The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy. EMBO Rep 2008; In press.
14. Mizushima N, Noda T, Ohsumi Y. Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway. EMBO J 1999; 18:3888-96.
15. Ichimura Y, Kirisako T, Takao T, Satomi Y, Shimonishi Y, Ishihara N, et al. A ubiquitin-like system mediates protein lipidation. Nature 2000; 408:488-92.
16. Fujioka Y, Noda NN, Fujii K, Yoshimoto K, Ohsumi Y, Inagaki F. In vitro reconstitution of plant Atg8 and Atg12 conjugation systems essential for autophagy. J Biol Chem 2008; 283:1921-8.
17. Shao Y, Gao Z, Feldman T, Jiang X. Stimulation of ATG12-ATG5 conjugation by ribonucleic acid. Autophagy 2007; 3:10-6.
18. Hanada T, Noda NN, Satomi Y, Ichimura Y, Fujioka Y, Takao T, et al. The ATG12-ATG5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J Biol Chem 2007.
19. Ichimura Y, Imamura Y, Emoto K, Umeda M, Noda T, Ohsumi Y. In vivo and in vitro reconstitution of Atg8 conjugation essential for autophagy. J Biol Chem 2004; 279:40584-92.
20. Kim J, Huang W-P, Klionsky DJ. Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex. J Cell Biol 2001; 152:51-64.